| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| rp145642-10μg | 10μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $99.90 | |
| rp145642-50μg | 50μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $409.90 | |
| rp145642-100μg | 100μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $599.90 | |
| rp145642-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $3,499.90 |
Animal Free, ≥95% (SDS-PAGE), Active, 293F, C-His tag, 28-229 aa
| Product Name | Recombinant Human Ephrin-B2 Protein, ≥95% (SDS-PAGE), high purity |
|---|---|
| Synonyms | EPH-related receptor tyrosine kinase ligand 5 | HTK ligand | HTK-L | LERK-5 | EFNB2 | ELF-2 | ephrin B2 | EphrinB2 | Ephrin-B2 | EPLG5 | HTKL | Htk-L | LERK5 | LERK-5 | NLERK-1 | Eph related receptor tyrosine kinase ligand 5 | Ligand of eph related kinase |
| Grade | ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free |
| Product Description | Purity |
| Specifications & Purity | ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥95%(SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Bioactivity | Immobilized Recombinant Human Ephrin-B2 Protein (rp145642) at 1.0 μg/mL can bind Human EPHB4 with the ED50 for this effect is 504.8 ng/mL. |
| Endotoxin Concentration | <0.1 EU/μg |
| Expression System | HEK293 |
| Species | Human |
| Amino Acids | 28-229 aa |
| Sequence | IVLEPIYWNSSNSKFLPGQGLVLYPQIGDKLDIICPKVDSKTVGQYEYYKVYMVDKDQADRCTIKKENTPLLNCAKPDQDIKFTIKFQEFSPNLWGLEFQKNKDYYIISTSNGSLEGLDNQEGGVCQTRAMKILMKVGQDASSAGSTRNKDPTRRPELEAGTNGRSSTTSPFVKPNPGSSTDGNSAGHSGNNILGSEVALFAHHHHHH |
| Protein Tag | C-His |
| Accession # | P52799 |
| Predicted molecular weight | 23 kDa |
| SDS-PAGE | 30 - 40 kDa, under reducing conditions; 30 - 40 kDa, under non-reducing conditions. |
Recombinant Human Ephrin-B2 Protein (rp145642) - Protein Bioactivity
Immobilized Recombinant Human Ephrin-B2 Protein (rp145642) at 1.0 μg/mL can bind Human EPHB4 with the ED
₅₀
for this effect is 504.8 ng/mL.
Recombinant Human Ephrin-B2 Protein (rp145642) - SDS-PAGE
3 μg/lane of Recombinant Human Ephrin-B2 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 30 - 40 kDa.
| Form | Lyophilized |
|---|---|
| Reconstitution | Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml. |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at -20~-80℃ for more than 1 year. Upon delivery aliquot. Avoid freeze/thaw cycle. |
Enter Lot Number to search for COA:
| 1. Bowden TA, Aricescu AR, Nettleship JE, Siebold C, Rahman-Huq N, Owens RJ, Stuart DI, Jones EY. (2009) Structural plasticity of eph receptor A4 facilitates cross-class ephrin signaling.. Structure, 17 (10): (1386-97). [PMID:19836338] |
| 2. Bennett, B D BD and 6 more authors.. (1995) Molecular cloning of a ligand for the EPH-related receptor protein-tyrosine kinase Htk.. Proceedings of the National Academy of Sciences of the United States of America, (14): [PMID:7534404] |
| 3. Cerretti, D P DP and 9 more authors.. (1995) Isolation of LERK-5: a ligand of the eph-related receptor tyrosine kinases.. Molecular immunology, [PMID:8559144] |
| 4. Vogt, T T and 7 more authors.. (1998) Overexpression of Lerk-5/Eplg5 messenger RNA: a novel marker for increased tumorigenicity and metastatic potential in human malignant melanomas.. Clinical cancer research : an official journal of the American Association for Cancer Research, [PMID:9533549] |
| 5. Füller, Tim T, Korff, Thomas T, Kilian, Adrienne A, Dandekar, Gudrun G and Augustin, Hellmut G HG.. (2003) Forward EphB4 signaling in endothelial cells controls cellular repulsion and segregation from ephrinB2 positive cells.. Journal of cell science, (15): [PMID:12734395] |
| 6. Dunham, A A and 125 more authors.. (2004) The DNA sequence and analysis of human chromosome 13.. Nature, (1): [PMID:15057823] |
| 7. Bonaparte, Matthew I MI and 10 more authors.. (2005) Ephrin-B2 ligand is a functional receptor for Hendra virus and Nipah virus.. Proceedings of the National Academy of Sciences of the United States of America, (26): [PMID:15998730] |
| 8. Negrete, Oscar A OA and 6 more authors.. (2005) EphrinB2 is the entry receptor for Nipah virus, an emergent deadly paramyxovirus.. Nature, (21): [PMID:16007075] |
| 9. Negrete, Oscar A OA and 9 more authors.. (2006) Two key residues in ephrinB3 are critical for its use as an alternative receptor for Nipah virus.. PLoS pathogens, [PMID:16477309] |
| 10. Chrencik, Jill E JE and 9 more authors.. (2006) Structural and biophysical characterization of the EphB4*ephrinB2 protein-protein interaction and receptor specificity.. The Journal of biological chemistry, (22): [PMID:16867992] |
| 11. Bishop, Kimberly A KA and 11 more authors.. (2007) Identification of Hendra virus G glycoprotein residues that are critical for receptor binding.. Journal of virology, [PMID:17376907] |
| 12. Bowden, Thomas A TA and 5 more authors.. (2008) Structural basis of Nipah and Hendra virus attachment to their cell-surface receptor ephrin-B2.. Nature structural & molecular biology, [PMID:18488039] |
| 13. Qin, Haina H and 5 more authors.. (2010) Structural characterization of the EphA4-Ephrin-B2 complex reveals new features enabling Eph-ephrin binding promiscuity.. The Journal of biological chemistry, (1): [PMID:19875447] |
| 14. Okumura, Fumihiko F and 7 more authors.. (2017) Ubiquitin ligase SPSB4 diminishes cell repulsive responses mediated by EphB2.. Molecular biology of the cell, (15): [PMID:28931592] |
