| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| rp169607-10μg (Trial Size) Apply for free trial size（?） Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free! | 10μg | In stock | $99.90 | |
| rp169607-50μg | 50μg | In stock | $369.90 | |
| rp169607-100μg | 100μg | In stock | $599.90 | |
| rp169607-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $4,999.90 |
Animal Free, >90% (SDS-PAGE), Active, 293F cell, C-His tag, 1-652 aa
| Product Name | Recombinant Human ErbB2/HER2 Protein, >90% (SDS-PAGE), high purity |
|---|---|
| Synonyms | CD340 antigen | CD340 | c-erb B2/neu protein | EC 2.7.10 | EGFR2 | ErbB2 | HER2 | HER-2 | HER2EC 2.7.10.1 | herstatin | Metastatic lymph node gene 19 protein | MLN 19 | MLN19 | Neu Oncogene | NEUHER-2/neu | neuroblastoma/glioblastoma derived oncogene homo |
| Grade | ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free |
| Product Description | Purity:>90%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. ErbB2 is widely expressed in epithelial cells and has also been found to be over-expressed in a large number of breast carcinomas. Among ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other members of the ErbB family (ErbB1 (EGFR), ErbB3, ErbB4) to form higher affinity signaling complexes. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. Interestingly, this same three amino acid signal has also been found in ErbB1 and ErbB4. Phosphoinositide 3-kinase has been shown to play a role in ErbB2 signal transduction. The cytoplasmic domain of ErbB2 has been shown to associate with beta-catenin and plakoglobin. Human ErbB2 consists of 1255 amino acids (aa) with a 21 aa signal sequence, a 631 aa extracellular domain, a 23 aa transmembrane region, and a 580 aa cytoplasmic domain. ErbB2 can be shed from the cell surface by proteolytic cleavage by an unidentified protease. ErbB2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation. |
| Specifications & Purity | ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥90%(SDS-PAGE) |
| Purity | >90% (SDS-PAGE) |
| Bioactivity | Pertuzumab (anti-HER2) (P412000) at 1.0 ug/mL can bind biotinylated Human ErbB2/HER2 Protein (rp169607), The EC50 for this effect is 25.99 ng/mL. |
| Endotoxin Concentration | ＜1.0 EU/μg |
| Expression System | HEK293 |
| Amino Acids | 1-652 aa |
| Sequence | MELAALCRWGLLLALLPPGAASTQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLAC |
| Protein Tag | C-His |
| Predicted molecular weight | 70.2 kDa |
| SDS-PAGE | 81.0-109.6 kDa, under reducing conditions |
Recombinant Human ErbB2/HER2 Protein (rp169607) - Protein Bioactivity
Pertuzumab (anti-HER2) (P412000) at 1.0 ug/ml can bind biotinylated Human ErbB2/HER2 Protein (rp169607), The EC₅₀ for this effect is 25.99 ng/mL.
Recombinant Human ErbB2/HER2 Protein (rp169607) - SDS-PAGE
3 μg/lane of Recombinant Human ErbB2/HER2 Protein was resolved with SDS-PAGE under reducing (R) conditions and visualized by Coomassie® Blue staining, showing a band at 81.0-109.6 kDa under reducing conditions.
| Form | Lyophilized |
|---|---|
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilu |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at -20°C for 1 year. Avoid freeze / thaw cycle. |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303504 | Certificate of Analysis | Mar 22, 2024 | rp169607 |
| ZJ24F0303505 | Certificate of Analysis | Mar 22, 2024 | rp169607 |
| ZJ24F0303506 | Certificate of Analysis | Mar 22, 2024 | rp169607 |
| ZJ24R0300334 | Certificate of Analysis | Mar 15, 2024 | rp169607 |
