Recombinant Human ErbB2/HER2 Protein, >90% (SDS-PAGE), high purity

Features and benefits
  • Expression System: HEK293
  • Protein Tag: C-His
  • Bioactivity: Pertuzumab (anti-HER2) (P412000) at 1.0 ug/mL can bind biotinylated Human ErbB2/HER2 Protein (rp169607), The EC50 for this effect is 25.99 ng/mL.
  • Endotoxin Concentration: <1.0 EU/μg
Item Number
rp169607
Grouped product items
SKUSizeAvailabilityPrice Qty
rp169607-10μg (Trial Size)
Apply for free trial size(?)
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10μg
In stock
$99.90
rp169607-50μg
50μg
In stock
$369.90
rp169607-100μg
100μg
In stock
$599.90
rp169607-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$4,999.90

Animal Free, >90% (SDS-PAGE), Active, 293F cell, C-His tag, 1-652 aa

Basic Description

Product NameRecombinant Human ErbB2/HER2 Protein, >90% (SDS-PAGE), high purity
GradeActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free
Product Description

Purity:>90%, by SDS-PAGE visualized with Coomassie® Blue Staining.

Description:

ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. ErbB2 is widely expressed in epithelial cells and has also been found to be over-expressed in a large number of breast carcinomas. Among ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other members of the ErbB family (ErbB1 (EGFR), ErbB3, ErbB4) to form higher affinity signaling complexes. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. Interestingly, this same three amino acid signal has also been found in ErbB1 and ErbB4. Phosphoinositide 3-kinase has been shown to play a role in ErbB2 signal transduction. The cytoplasmic domain of ErbB2 has been shown to associate with beta-catenin and plakoglobin. Human ErbB2 consists of 1255 amino acids (aa) with a 21 aa signal sequence, a 631 aa extracellular domain, a 23 aa transmembrane region, and a 580 aa cytoplasmic domain. ErbB2 can be shed from the cell surface by proteolytic cleavage by an unidentified protease. ErbB2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation.


Specifications & PurityActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥90%(SDS-PAGE)
Purity>90% (SDS-PAGE)
BioactivityPertuzumab (anti-HER2) (P412000) at 1.0 ug/mL can bind biotinylated Human ErbB2/HER2 Protein (rp169607), The EC50 for this effect is 25.99 ng/mL.
Endotoxin Concentration<1.0 EU/μg
Expression SystemHEK293
Amino Acids1-652 aa
SequenceMELAALCRWGLLLALLPPGAASTQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLAC
Protein TagC-His
Predicted molecular weight70.2 kDa
SDS-PAGE81.0-109.6 kDa, under reducing conditions

Images

Recombinant Human ErbB2/HER2 Protein (rp169607) - Protein Bioactivity
Pertuzumab (anti-HER2) (P412000) at 1.0 ug/ml can bind biotinylated Human ErbB2/HER2 Protein (rp169607), The EC₅₀ for this effect is 25.99 ng/mL.

Recombinant Human ErbB2/HER2 Protein (rp169607) - SDS-PAGE
3 μg/lane of Recombinant Human ErbB2/HER2 Protein was resolved with SDS-PAGE under reducing (R) conditions and visualized by Coomassie® Blue staining, showing a band at 81.0-109.6 kDa under reducing conditions.

Product Specifications

FormLyophilized
ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilu
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -20°C for 1 year. Avoid freeze / thaw cycle.

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4 results found

Lot NumberCertificate TypeDateItem
ZJ24F0303504Certificate of AnalysisMar 22, 2024 rp169607
ZJ24F0303505Certificate of AnalysisMar 22, 2024 rp169607
ZJ24F0303506Certificate of AnalysisMar 22, 2024 rp169607
ZJ24R0300334Certificate of AnalysisMar 15, 2024 rp169607

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