Recombinant Human Erythropoietin/EPO Protein, >95% (SDS-PAGE), high purity

Features and benefits
  • Expression System: HEK293
  • Protein Tag: C-His
  • Bioactivity: Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.39 ng/mL.
  • Endotoxin Concentration: <1.0 EU/μg
Item Number
rp156337
Grouped product items
SKUSizeAvailabilityPrice Qty
rp156337-10μg (Trial Size)
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10μg
In stock
$89.90
rp156337-50μg
50μg
In stock
$259.90
rp156337-100μg
100μg
In stock
$399.90
rp156337-1mg
1mg
Available within 8-12 weeks(?)
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$1,499.90

Animal Free, >95% (SDS-PAGE), Active, 293F cell, His tag, 28-193 aa

Basic Description

Product NameRecombinant Human Erythropoietin/EPO Protein, >95% (SDS-PAGE), high purity
GradeActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance
Product Description

Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining
Description:
Human erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

Specifications & PurityActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE), Lot by Lot
Purity>95% (SDS-PAGE)
BioactivityMeasured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.39 ng/mL.
Endotoxin Concentration<1.0 EU/μg
Expression SystemHEK293
Amino Acids28-193 aa
SequenceAPPRLICDSRVLERYLLEAKEAENITTGCAEHCSLNENITVPDTKVNFYAWKRMEVGQQAVEVWQGLALLSEAVLRGQALLVNSSQPWEPLQLHVDKAVSGLRSLTTLLRALGAQKEAISPPDAASAAPLRTITADTFRKLFRVYSNFLRGKLKLYTGEACRTGDRENLYFQSHHHHHH
Protein TagC-His
Predicted molecular weight20.1 kDa
SDS-PAGE37.6 kDa, under reducing condition; 37.1 kDa, under non-reducing condition

Images

Recombinant Human EPO Protein (rp156337) - Protein Bioactivity
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED₅₀ for this effect is 0.39 ng/mL.

Recombinant Human EPO Protein (rp156337) - SDS-PAGE
3 μg/lane of Recombinant Human EPO Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 37.6 kDa under reducing conditions and 37.1 kDa under non-reducing conditions.

Product Specifications

FormLyophilized
ConcentrationLot by Lot
ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilu
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -20°C stable up to 1 year. Avoid freeze / thaw cycle.

Certificates

Certificate of Analysis(COA)

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4 results found

Lot NumberCertificate TypeDateItem
ZJ24F0303304Certificate of AnalysisMar 22, 2024 rp156337
ZJ24F0303303Certificate of AnalysisMar 22, 2024 rp156337
ZJ24F0303305Certificate of AnalysisMar 22, 2024 rp156337
ZJ24R0300317Certificate of AnalysisMar 08, 2024 rp156337

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