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Recombinant Human FABP4 Protein, ＞ 95%(SDS-PAGE), high purity

≥95%(SDS-PAGE)

Features and benefits

Expression System: HEK293
Accession #: P15090
Protein Tag: N-His
Bioactivity: The binding activity of FABP4 with ACTB.
Endotoxin Concentration: ＜1.0 EU/μg

Item Number

rp145772

Grouped product items
SKU	Size	Availability	Price
rp145772-10μg	10μg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$129.90
rp145772-50μg	50μg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$579.90
rp145772-100μg	100μg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$869.90
rp145772-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$4,999.90

Animal Free, > 95%(SDS-PAGE), Active, 293F, His tag, 2-132 aa

View related series

Click to view protein information: FABP4

Accession#:P15090 FABP4 Gene ID:2167

Basic Description

Product Name	Recombinant Human FABP4 Protein, ＞ 95%(SDS-PAGE), high purity
Synonyms	3T3-L1 lipid-binding protein; 422/aP2; A-FABP; Adipocyte; Adipocyte lipid binding protein; Adipocyte lipid-binding protein; Adipocyte protein AP2; Adipocyte-type fatty acid-binding protein; AFABP; ALBP; ALBP/Ap2; aP2; Epididymis secretory protein Li 104;
Grade	ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free
Product Description	Purity >95% SDS-PAGE Function Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.
Specifications & Purity	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥95%(SDS-PAGE)
Purity	＞ 95%(SDS-PAGE)
Bioactivity	The binding activity of FABP4 with ACTB.
Endotoxin Concentration	＜1.0 EU/μg
Expression System	HEK293
Species	Human
Amino Acids	2-132 aa
Sequence	CDAFVGTWKLVSSENFDDYMKEVGVGFATRKVAGMAKPNMIISVNGDVITIKSESTFKNTEISFILGQEFDEVTADDRKVKSTITLDGGVLVHVQKWDGKSTTIKRKREDDKLVVECVMKGVTSTRVYERA
Protein Tag	N-His
Accession #	P15090
Predicted molecular weight	16.2 kDa
SDS-PAGE	16 kDa under reducing condition

Images

Recombinant Human FABP4 Protein (rp145772) - Protein Bioactivity
Fatty Acid Binding Protein 4, Adipocyte (FABP4) encodes the fatty acid binding protein found in adipocytes. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. Besides, Actin Beta has been identified as an interactor of FABP4, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FABP4 and recombinant human ACTb. The EC₅₀ for this effect is less than 4.23 ug/mL.

Recombinant Human FABP4 Protein (rp145772) - SDS-PAGE
3μg/lane of Recombinant Human FABP4 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 13.2 kDa.

Product Specifications

Form	Lyophilized
Reconstitution	Add 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL.
Storage Temp	Store at -80°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Avoid repeated freeze/thaw cycles. Store at 2-8℃ for one month. Aliquot and store at -80℃ for 12 months.

Certificates

Certificate of Analysis(COA)

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3 results found

Lot Number	Certificate Type	Date	Item
ZJ23F0800875	Certificate of Analysis	Aug 23, 2023	rp145772
ZJ23F0800876	Certificate of Analysis	Aug 23, 2023	rp145772
ZJ23F0800877	Certificate of Analysis	Aug 23, 2023	rp145772

References

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2. Liu X, Huang X, Lin W, Wang D, Diao Y, Li H, Hui X, Wang Y, Xu A, Wu D et al.. (2011) New aromatic substituted pyrazoles as selective inhibitors of human adipocyte fatty acid-binding protein.. Bioorg Med Chem Lett, 21 (10): (2949-52). [PMID:21481589]
3. Oddi S, Scipioni L, Totaro A, Angelucci C, Dufrusine B, Sabatucci A, Tortolani D, Coletta I, Alisi MA, Polenzani L et al.. (2019) The anti-inflammatory agent bindarit acts as a modulator of fatty acid-binding protein 4 in human monocytic cells.. Sci Rep, 9 (1): (15155). [PMID:31641194]
4. Haiyan Cai,Guirui Yan,Xiaodong Zhang,Olena Gorbenko,Heyao Wang,Weiliang Zhu. (2010-05-18) Discovery of highly selective inhibitors of human fatty acid binding protein 4 (FABP4) by virtual screening.. Bioorganic & medicinal chemistry letters, 20 ((12)): (3675-3679). [PMID:20471252]
5. Lehmann F, Haile S, Axen E, Medina C, Uppenberg J, Svensson S, Lundbäck T, Rondahl L, Barf T.. (2004) Discovery of inhibitors of human adipocyte fatty acid-binding protein, a potential type 2 diabetes target.. Bioorg Med Chem Lett, 14 (17): (4445-4448). [PMID:15357969]
6. Barf T, Lehmann F, Hammer K, Haile S, Axen E, Medina C, Uppenberg J, Svensson S, Rondahl L, Lundbäck T.. (2009) N-Benzyl-indolo carboxylic acids: Design and synthesis of potent and selective adipocyte fatty-acid binding protein (A-FABP) inhibitors.. Bioorg Med Chem Lett, 19 (6): (1745-1748). [PMID:19217286]
7. Cai H, Liu Q, Gao D, Wang T, Chen T, Yan G, Chen K, Xu Y, Wang H, Li Y, Zhu W.. (2015) Novel fatty acid binding protein 4 (FABP4) inhibitors: virtual screening, synthesis and crystal structure determination.. Eur J Med Chem, 90 (241-250). [PMID:25461324]
8. Zhou Y, Nie T, Zhang Y, Song M, Li K, Ding M, Ding K, Wu D, Xu Y.. (2016) The discovery of novel and selective fatty acid binding protein 4 inhibitors by virtual screening and biological evaluation.. Bioorg Med Chem, 24 (18): (4310-4317). [PMID:27460668]
9. Kühne H, Obst-Sander U, Kuhn B, Conte A, Ceccarelli SM, Neidhart W, Rudolph MG, Ottaviani G, Gasser R, So SS, Li S, Zhang X, Gao L, Myers M.. (2016) Design and synthesis of selective, dual fatty acid binding protein 4 and 5 inhibitors.. Bioorg Med Chem Lett, 26 (20): (5092-5097). [PMID:27658368]
10. Gao DD, Dou HX, Su HX, Zhang MM, Wang T, Liu QF, Cai HY, Ding HP, Yang Z, Zhu WL, Xu YC, Wang HY, Li YX.. (2018) From hit to lead: Structure-based discovery of naphthalene-1-sulfonamide derivatives as potent and selective inhibitors of fatty acid binding protein 4.. Eur J Med Chem, 154 (44-59). [PMID:29775936]
11. Yan S, Elmes MW, Tong S, Hu K, Awwa M, Teng GYH, Jing Y, Freitag M, Gan Q, Clement T, Wei L, Sweeney JM, Joseph OM, Che J, Carbonetti GS, Wang L, Bogdan DM, Falcone J, Smietalo N, Zhou Y, Ralph B, Hsu HC, Li H, Rizzo RC, Deutsch DG, Kaczocha M, Ojima I.. (2018) SAR studies on truxillic acid mono esters as a new class of antinociceptive agents targeting fatty acid binding proteins.. Eur J Med Chem, 154 (233-252). [PMID:29803996]
12. He Y, Dou H, Gao D, Wang T, Zhang M, Wang H, Li Y.. (2019) Identification of new dual FABP4/5 inhibitors based on a naphthalene-1-sulfonamide FABP4 inhibitor.. Bioorg Med Chem, 27 (19): (115015-115015). [PMID:31420256]
13. Oukoloff K, Lucero B, Francisco KR, Brunden KR, Ballatore C.. (2019) 1,2,4-Triazolo[1,5-a]pyrimidines in drug design.. Eur J Med Chem, 165 (332-346). [PMID:30703745]
14. Baxa, C A CA and 7 more authors.. (1989) Human adipocyte lipid-binding protein: purification of the protein and cloning of its complementary DNA.. Biochemistry, (31): [PMID:2481498]
15. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895]
16. Rosenow, Anja A and 6 more authors.. (2012) Resveratrol-induced changes of the human adipocyte secretion profile.. Journal of proteome research, (7): [PMID:22905912]

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