| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| rp145939-50μg | 50μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $189.90 | |
| rp145939-100μg | 100μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $229.90 | |
| rp145939-1mg | 1mg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $799.90 | |
| rp145939-500μg | 500μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $479.90 |
GMP, >95% SDS-PAGE, Active, E.coli , No tag, 134-288 aa
| Product Name | Recombinant Human FGF basic/FGF2/bFGF(155aa) Protein, ＞95% SDS-PAGE, high purity |
|---|---|
| Synonyms | Basic fibroblast growth factor Basic fibroblast growth factor bFGF BFGF FGF 2 FGF B FGF-2 Fgf2 FGF2 basic FGF2_HUMAN FGFB Fibroblast growth factor 2 Fibroblast growth factor 2 (basic) Fibroblast growth factor, basic HBGF 2 HBGF-2 HBGF2 HBGH 2 HBGH2 Hepari |
| Grade | ActiBioPure™, Azide Free, Bioactive, Carrier Free, GMP, High Performance |
| Product Description | Purity >95% SDS-PAGE. lyophilised from 0.22 µm filtered solution. Function Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107). Post-translational Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion. Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing. Recombinant human basic fibroblast growth factor (also known as basic FGF, bFGF, FGF2, FGF-beta, or heparin-binding growth factor), is a biologically active protein suitable for cell culture applications. bFGF regulates diverse processes such as cell proliferation, differentiation, survival, adhesion, motility, apoptosis, limb formation, and wound recovery. bFGF can be used in studies of angiogenesis, fibroblast mitosis, axonal outgrowth in PC-12 cells, receptor binding, and tyrosine phosphorylation. This strain is expressed in recombinant Escherichia coli, and after multi-step separation and purification, it is dissolved in 10mM PBS, 0.15 M NaCl (pH7.2) solution, filtered through a 0.22 μm filter membrane, and then freeze-dried to make a lyophilized powder. . |
| Specifications & Purity | ActiBioPure™, Bioactive, GMP, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE) |
| Purity | ＞95% SDS-PAGE |
| Bioactivity | Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <0.9 ng/mL. |
| Endotoxin Concentration | ＜0.2 EU/μg |
| Expression System | E.coli |
| Species | Human |
| Amino Acids | 134-288 aa |
| Sequence | MAAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS |
| Protein Tag | No tag |
| Protein Length | Full length protein |
| Accession # | P09038 |
| Source | Recombinant |
| Predicted molecular weight | 17 kDa |
Recombinant Human FGF basic/FGF2/bFGF(155aa) Protein (rp145939)-SDS-PAGE
2μg/lane of Recombinant Human FGF2 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 15.6 kDa.
Recombinant Human FGF basic/FGF2/bFGF(155aa) Protein (rp145939)-Protein Bioactivity
Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <0.9ng/mL.
| Form | Lyophilized |
|---|---|
| Reconstitution | It is recommended to briefly centrifuge before opening the cap to make the powder sink to the bottom of the tube, then dissolve it in sterilized deionized water to prepare a 100μg/ml stock solution, which can be stored stably for one week at 2°C ~ 8°C. Wh |
| Storage Temp | Store at -80°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Stable up to 24 months at -15°C and below, and up to 4 weeks at 2~8°C. Prepared as a stock solution of 100μg/ml, it can be stored stably for one week at 2~8℃. |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0400109 | Certificate of Analysis | Apr 07, 2023 | rp145939 |
| ZJ23F0400108 | Certificate of Analysis | Apr 06, 2023 | rp145939 |
| ZJ23F0400107 | Certificate of Analysis | Apr 06, 2023 | rp145939 |
| ZJ23F0400097 | Certificate of Analysis | Apr 06, 2023 | rp145939 |
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