Recombinant human FGF1 protein, >95% SDS-PAGE, high purity

Features and benefits
  • Expression System: E. coli
  • Accession #: P05230
  • Protein Tag: No tag
  • Bioactivity: Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line in the presence of heparin. The ED₅₀ for this effect is typically <0.5ng/mL.
  • Endotoxin Concentration: <0.1 EU/μg
Item Number
rp145907
Grouped product items
SKUSizeAvailabilityPrice Qty
rp145907-25μg
25μg
In stock
$69.90
rp145907-100μg
100μg
In stock
$249.90
rp145907-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,289.90

Animal Free, >95% SDS-PAGE, Active, E.coli, No tag, 16-155 aa

Basic Description

Product NameRecombinant human FGF1 protein, >95% SDS-PAGE, high purity
SynonymsAcidic fibroblast growth factor | aFGF | ECGF | Endothelial cell growth factor | FGF-1 | HBGF-1 | Heparin-binding growth factor 1
GradeActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance
Product Description

Purity

>95% as determined by SDS-PAGE and Coomassie blue staining

Function

The heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro.

Sequence similarities

Belongs to the heparin-binding growth factors family.

Cellular localization

Secreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1.


Specifications & PurityActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE)
Purity>95% SDS-PAGE
BioactivityMeasured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line in the presence of heparin. The ED₅₀ for this effect is typically <0.5ng/mL.
Endotoxin Concentration<0.1 EU/μg
Expression SystemE. coli
SpeciesHuman
Amino Acids16-155 aa
Protein TagNo tag
Protein LengthFull length protein
Accession #P05230
SourceRecombinant
Predicted molecular weight15.9 kDa

Images

Recombinant Human FGF1 Protein (rp145907)-Protein Bioactivity
Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line in the presence of heparin. The ED50 for this effect is typically <0.5ng/mL.

Recombinant Human FGF1 Protein (rp145907)-SDS-PAGE
3μg/lane of Recombinant Human FGF1 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 13.3 kDa.

Product Specifications

FormLyophilized
ReconstitutionAfter receiving lyophilized powder protein, centrifuge first, and then r econstitute at 100µg/mL in sterile PBS.
Storage TempStore at -20°C
Shipped InIce chest + Ice pads
Stability And StorageStable for 12 months from the date of receipt of the product under proper storage andhandling conditions. Avoid repeated freeze-thaw cycles.

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot NumberCertificate TypeDateItem
ZJ23F1101723Certificate of AnalysisNov 20, 2023 rp145907
ZJ23F1101722Certificate of AnalysisNov 20, 2023 rp145907
ZJ23F0400117Certificate of AnalysisApr 29, 2023 rp145907
ZJ23F0400116Certificate of AnalysisApr 29, 2023 rp145907

Related Documents

References

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