| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| rp145907-25μg | 25μg | In stock | $69.90 | |
| rp145907-100μg | 100μg | In stock | $249.90 | |
| rp145907-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,289.90 |
Animal Free, >95% SDS-PAGE, Active, E.coli, No tag, 16-155 aa
| Product Name | Recombinant human FGF1 protein, ＞95% SDS-PAGE, high purity |
|---|---|
| Synonyms | Acidic fibroblast growth factor aFGF Beta; endothelial cell growth factor Beta; endothelial cell growth factor; ECGF; ECGF beta; ECGF-beta; ECGFA; ECGFB; Endothelial Cell Growth Factor alpha; Endothelial Cell Growth Factor beta; FGF 1; FGF alpha; Fgf1; FG |
| Grade | ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High performance |
| Product Description | Purity >95% as determined by SDS-PAGE and Coomassie blue staining Function The heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro. Sequence similarities Belongs to the heparin-binding growth factors family. Cellular localization Secreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1. |
| Specifications & Purity | ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE) |
| Purity | ＞95% SDS-PAGE |
| Bioactivity | Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line in the presence of heparin. The ED₅₀ for this effect is typically <0.5ng/mL. |
| Endotoxin Concentration | < 0.1 EU/µg |
| Expression System | E. coli |
| Species | Human |
| Amino Acids | 16-155 aa |
| Sequence | FNLPPGNYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHIQLQLSAES VGEVYIKSTE TGQYLAMDTDGLLYGSQTPNEECLFLERLEENHYNTYISKKHAEKNWFVG LKKNGSCKRG PRTHYGQKAILFLPLPVSSD |
| Protein Tag | No tag |
| Protein Length | Full length protein |
| Accession # | P05230 |
| Source | Recombinant |
| Predicted molecular weight | 15.9 kDa |
Recombinant Human FGF1 Protein (rp145907)-Protein Bioactivity
Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line in the presence of heparin. The ED50 for this effect is typically <0.5ng/mL.
Recombinant Human FGF1 Protein (rp145907)-SDS-PAGE
3μg/lane of Recombinant Human FGF1 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 13.3 kDa.
| Form | Lyophilized |
|---|---|
| Reconstitution | After receiving lyophilized powder protein, centrifuge first, and then r econstitute at 100µg/mL in sterile PBS. |
| Storage Temp | Store at -20°C |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Stable for 12 months from the date of receipt of the product under proper storage andhandling conditions. Avoid repeated freeze-thaw cycles. |
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| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1101723 | Certificate of Analysis | Nov 20, 2023 | rp145907 |
| ZJ23F1101722 | Certificate of Analysis | Nov 20, 2023 | rp145907 |
| ZJ23F0400117 | Certificate of Analysis | Apr 29, 2023 | rp145907 |
| ZJ23F0400116 | Certificate of Analysis | Apr 29, 2023 | rp145907 |
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