Recombinant Human G-CSF Protein, ≥95% (SDS-PAGE), high purity

Features and benefits
  • Expression System: HEK293
  • Accession #: P09919
  • Protein Tag: C-His
  • Bioactivity: Measured in a cell proliferation assay using NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 1.05 pg/mL.
  • Endotoxin Concentration: <0.1 EU/μg
Item Number
rp146356
Grouped product items
SKUSizeAvailabilityPrice Qty
rp146356-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$139.90
rp146356-50μg
50μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$419.90
rp146356-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$669.90
rp146356-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$3,339.90

Animal Free, ≥95% (SDS-PAGE), Active, 293F, C-His tag, 1-204 aa

Basic Description

Product NameRecombinant Human G-CSF Protein, ≥95% (SDS-PAGE), high purity
SynonymsG-CSF | Pluripoietin
GradeActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance
Product Description

Purity
≥95% SDS-PAGE.
Endotoxin level
<0.1 EU/µg
Function
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.


Specifications & PurityActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE)
Purity≥95% (SDS-PAGE)
BioactivityMeasured in a cell proliferation assay using NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 1.05 pg/mL.
Endotoxin Concentration<0.1 EU/μg
Expression SystemHEK293
SpeciesHuman
Amino Acids1-204 aa
SequenceMAGPATQSPMKLMALQLLLWHSALWTVQEATPLGPASSLPQSFLLKCLEQVRKIQGDGAALQEKLCATYKLCHPEELVLLGHSLGIPWAPLSSCPSQALQLAGCLSQLHSGLFLYQGLLQALEGISPELGPTLDTLQLDVADFATTIWQQMEELGMAPALQPTQGAMPAFASAFQRRAGGVLVASHLQSFLEVSYRVLRHLAQPHHHHHH
Protein TagC-His
Accession #P09919
Predicted molecular weight23 kDa
SDS-PAGE17.5 kDa, under reducing conditions; 17.5 kDa, under non-reducing conditions.

Images

Recombinant Human G-CSF Protein (rp146356) - Protein Bioactivity
Measured in a cell proliferation assay using NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 1.05 pg/mL.

Recombinant Human G-CSF Protein (rp146356) - SDS-PAGE
3 μg/lane of Recombinant Human G-CSF Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 17.5 kDa.

Product Specifications

FormLyophilized
ReconstitutionReconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -20~-80℃ for more than 1 year. Upon delivery aliquot. Avoid freeze/thaw cycle.

Certificates

Certificate of Analysis(COA)

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3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0607424Certificate of AnalysisJun 28, 2024 rp146356
ZJ24F0607423Certificate of AnalysisJun 28, 2024 rp146356
ZJ24F0607422Certificate of AnalysisJun 28, 2024 rp146356

Related Documents

References

1. Tamada T, Honjo E, Maeda Y, Okamoto T, Ishibashi M, Tokunaga M, Kuroki R.  (2006)  Homodimeric cross-over structure of the human granulocyte colony-stimulating factor (GCSF) receptor signaling complex..  Proc Natl Acad Sci USA,  103  (9): (3135-40).  [PMID:16492764]
2. Zink, T T, Ross, A A, Ambrosius, D D, Rudolph, R R and Holak, T A TA..  (1992)  Secondary structure of human granulocyte colony-stimulating factor derived from NMR spectroscopy..  FEBS letters,    (21):   [PMID:1281794]
3. Souza, L M LM and 14 more authors..  (1986)  Recombinant human granulocyte colony-stimulating factor: effects on normal and leukemic myeloid cells..  Science (New York, N.Y.),    (4):   [PMID:2420009]
4. Nagata, S S and 8 more authors..  (1986)  The chromosomal gene structure and two mRNAs for human granulocyte colony-stimulating factor..  The EMBO journal,      [PMID:2423327]
5. Nagata, S S and 9 more authors..  (1986)  Molecular cloning and expression of cDNA for human granulocyte colony-stimulating factor..  Nature,      [PMID:3484805]
6. Devlin, J J JJ and 5 more authors..  (1987)  Expression of granulocyte colony-stimulating factor by human cell lines..  Journal of leukocyte biology,      [PMID:3494801]
7. Zink, T T and 5 more authors..  (1994)  Structure and dynamics of the human granulocyte colony-stimulating factor determined by NMR spectroscopy. Loop mobility in a four-helix-bundle protein..  Biochemistry,    (19):   [PMID:7518249]
8. Hill, C P CP, Osslund, T D TD and Eisenberg, D D..  (1993)  The structure of granulocyte-colony-stimulating factor and its relationship to other growth factors..  Proceedings of the National Academy of Sciences of the United States of America,    (1):   [PMID:7685117]
9. Haniu, M M and 5 more authors..  (1995)  Extracellular domain of granulocyte-colony stimulating factor receptor. Interaction with its ligand and identification of a domain in close proximity of ligand-binding region..  Archives of biochemistry and biophysics,    (20):   [PMID:8554326]
10. Aritomi, M M and 5 more authors..  (1999)  Atomic structure of the GCSF-receptor complex showing a new cytokine-receptor recognition scheme..  Nature,    (14):   [PMID:10537111]
11. Jin, Pei P and 7 more authors..  (2004)  PCR isolation and cloning of novel splice variant mRNAs from known drug target genes..  Genomics,      [PMID:15028279]
12. Zody, Michael C MC and 73 more authors..  (2006)  DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage..  Nature,    (20):   [PMID:16625196]

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