Recombinant Human G-CSF Protein, ≥95% (SDS-PAGE), high purity
Synonyms
G-CSF | Pluripoietin
Grade
ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance
Product Description
Purity ≥95% SDS-PAGE. Endotoxin level <0.1 EU/µg Function Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
17.5 kDa, under reducing conditions; 17.5 kDa, under non-reducing conditions.
Images
Recombinant Human G-CSF Protein (rp146356) - Protein Bioactivity Measured in a cell proliferation assay using NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 1.05 pg/mL.
Recombinant Human G-CSF Protein (rp146356) - SDS-PAGE 3 μg/lane of Recombinant Human G-CSF Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 17.5 kDa.
Product Specifications
Form
Lyophilized
Reconstitution
Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20~-80℃ for more than 1 year. Upon delivery aliquot. Avoid freeze/thaw cycle.
Certificates
Certificate of Analysis(COA)
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1.Tamada T, Honjo E, Maeda Y, Okamoto T, Ishibashi M, Tokunaga M, Kuroki R. (2006) Homodimeric cross-over structure of the human granulocyte colony-stimulating factor (GCSF) receptor signaling complex.. Proc Natl Acad Sci USA, 103 (9):(3135-40). [PMID:16492764]
2.Zink, T T, Ross, A A, Ambrosius, D D, Rudolph, R R and Holak, T A TA.. (1992) Secondary structure of human granulocyte colony-stimulating factor derived from NMR spectroscopy.. FEBS letters, (21):[PMID:1281794]
3.Souza, L M LM and 14 more authors.. (1986) Recombinant human granulocyte colony-stimulating factor: effects on normal and leukemic myeloid cells.. Science (New York, N.Y.), (4):[PMID:2420009]
4.Nagata, S S and 8 more authors.. (1986) The chromosomal gene structure and two mRNAs for human granulocyte colony-stimulating factor.. The EMBO journal, [PMID:2423327]
5.Nagata, S S and 9 more authors.. (1986) Molecular cloning and expression of cDNA for human granulocyte colony-stimulating factor.. Nature, [PMID:3484805]
6.Devlin, J J JJ and 5 more authors.. (1987) Expression of granulocyte colony-stimulating factor by human cell lines.. Journal of leukocyte biology, [PMID:3494801]
7.Zink, T T and 5 more authors.. (1994) Structure and dynamics of the human granulocyte colony-stimulating factor determined by NMR spectroscopy. Loop mobility in a four-helix-bundle protein.. Biochemistry, (19):[PMID:7518249]
8.Hill, C P CP, Osslund, T D TD and Eisenberg, D D.. (1993) The structure of granulocyte-colony-stimulating factor and its relationship to other growth factors.. Proceedings of the National Academy of Sciences of the United States of America, (1):[PMID:7685117]
9.Haniu, M M and 5 more authors.. (1995) Extracellular domain of granulocyte-colony stimulating factor receptor. Interaction with its ligand and identification of a domain in close proximity of ligand-binding region.. Archives of biochemistry and biophysics, (20):[PMID:8554326]
10.Aritomi, M M and 5 more authors.. (1999) Atomic structure of the GCSF-receptor complex showing a new cytokine-receptor recognition scheme.. Nature, (14):[PMID:10537111]
11.Jin, Pei P and 7 more authors.. (2004) PCR isolation and cloning of novel splice variant mRNAs from known drug target genes.. Genomics, [PMID:15028279]
12.Zody, Michael C MC and 73 more authors.. (2006) DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage.. Nature, (20):[PMID:16625196]
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