Recombinant Human G-CSF Protein, ≥95% (SDS-PAGE), high purity
Synonyms
G-CSF | Pluripoietin
Grade
ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance
Product Description
Purity ≥95% SDS-PAGE. Endotoxin level <0.1 EU/µg Function Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
17.5 kDa, under reducing conditions; 17.5 kDa, under non-reducing conditions.
Images
Recombinant Human G-CSF Protein (rp146356) - Protein Bioactivity Measured in a cell proliferation assay using NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 1.05 pg/mL.
Recombinant Human G-CSF Protein (rp146356) - SDS-PAGE 3 μg/lane of Recombinant Human G-CSF Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 17.5 kDa.
Product Specifications
Form
Lyophilized
Reconstitution
Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20~-80℃ for more than 1 year. Upon delivery aliquot. Avoid freeze/thaw cycle.
Certificates
Certificate of Analysis(COA)
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
1.Tamada T, Honjo E, Maeda Y, Okamoto T, Ishibashi M, Tokunaga M, Kuroki R. (2006) Homodimeric cross-over structure of the human granulocyte colony-stimulating factor (GCSF) receptor signaling complex.. Proc Natl Acad Sci USA, 103 (9):(3135-40). [PMID:16492764]
2.Zink, T T, Ross, A A, Ambrosius, D D, Rudolph, R R and Holak, T A TA.. (1992) Secondary structure of human granulocyte colony-stimulating factor derived from NMR spectroscopy.. FEBS letters, (21):[PMID:1281794]
3.Souza, L M LM and 14 more authors.. (1986) Recombinant human granulocyte colony-stimulating factor: effects on normal and leukemic myeloid cells.. Science (New York, N.Y.), (4):[PMID:2420009]
4.Nagata, S S and 8 more authors.. (1986) The chromosomal gene structure and two mRNAs for human granulocyte colony-stimulating factor.. The EMBO journal, [PMID:2423327]
5.Nagata, S S and 9 more authors.. (1986) Molecular cloning and expression of cDNA for human granulocyte colony-stimulating factor.. Nature, [PMID:3484805]
6.Devlin, J J JJ and 5 more authors.. (1987) Expression of granulocyte colony-stimulating factor by human cell lines.. Journal of leukocyte biology, [PMID:3494801]
7.Zink, T T and 5 more authors.. (1994) Structure and dynamics of the human granulocyte colony-stimulating factor determined by NMR spectroscopy. Loop mobility in a four-helix-bundle protein.. Biochemistry, (19):[PMID:7518249]
8.Hill, C P CP, Osslund, T D TD and Eisenberg, D D.. (1993) The structure of granulocyte-colony-stimulating factor and its relationship to other growth factors.. Proceedings of the National Academy of Sciences of the United States of America, (1):[PMID:7685117]
9.Haniu, M M and 5 more authors.. (1995) Extracellular domain of granulocyte-colony stimulating factor receptor. Interaction with its ligand and identification of a domain in close proximity of ligand-binding region.. Archives of biochemistry and biophysics, (20):[PMID:8554326]
10.Aritomi, M M and 5 more authors.. (1999) Atomic structure of the GCSF-receptor complex showing a new cytokine-receptor recognition scheme.. Nature, (14):[PMID:10537111]
11.Jin, Pei P and 7 more authors.. (2004) PCR isolation and cloning of novel splice variant mRNAs from known drug target genes.. Genomics, [PMID:15028279]
12.Zody, Michael C MC and 73 more authors.. (2006) DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage.. Nature, (20):[PMID:16625196]
Solution Calculators
Molarity Calculator
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.