Recombinant Human GDNF Protein, ≥97% (SDS-PAGE), high purity

Features and benefits
  • Expression System: HEK293
  • Protein Tag: No tag
  • Bioactivity: 1. Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED50 for this effect is typically 3-20 ng/mL in the presence of Recombinant Human GFR alpha-1/GDNF R alpha-1 His Chimera. 2. Immobilized Recombinant Human GDNF Protein
  • Endotoxin Concentration: <1.0 EU/μg
Item Number
rp146397
Grouped product items
SKUSizeAvailabilityPrice Qty
rp146397-10μg
10μg
In stock
$139.90
rp146397-50μg
50μg
In stock
$649.90
rp146397-100μg
100μg
In stock
$1,069.90
rp146397-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$4,449.90

Animal Free, ≥97% (SDS-PAGE), Active, HEK293, No tag, 83-185 aa

Basic Description

Product NameRecombinant Human GDNF Protein, ≥97% (SDS-PAGE), high purity
SynonymsAstrocyte-derived trophic factor | ATF | ATF1 | ATF2 | GDNF | Glial cell derived neurotrophic factor | Glial cell line derived neurotrophic factor | Glial cell line-derived neurotrophic factor | HFB1-GDNF | HGDNF | HSCR3
GradeActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance
Product Description

Purity
≥97% (SDS-PAGE)
Endotoxin level
<1.0 EU/μg
Function
Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

Specifications & PurityActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥97%(SDS-PAGE)
Purity≥97% (SDS-PAGE)
Bioactivity1. Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED50 for this effect is typically 3-20 ng/mL in the presence of Recombinant Human GFR alpha-1/GDNF R alpha-1 His Chimera. 2. Immobilized Recombinant Human GDNF Protein
Endotoxin Concentration<1.0 EU/μg
Expression SystemHEK293
SpeciesHuman
Amino Acids83-185 aa
Protein TagNo tag
Predicted molecular weight15.1 KDa
SDS-PAGE15.6 kDa, under reducing conditions; 32.6 kDa, under non-reducing conditions.

Images

Recombinant Human GDNF Protein (rp146397) - Protein Bioactivity
Immobilized Recombinant Human GDNF Protein (rp146397) at 2 μg/mL can bind biotinylated Recombinant Human GFRA1/GFRα1/GDNFRA Protein, the EC₅₀ is 15.0 - 48.0 ng/mL.

Recombinant Human GDNF Protein (rp146397) - SDS-PAGE
3 μg/lane of Recombinant Human GDNF Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 15.6 kDa under reducing conditions and 32.6 kDa under non-reducing conditions.

Product Specifications

FormLyophilized
ReconstitutionIt is recommended that sterile water be added to the vial to prepare astock solution of 0.25mg/mL.
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageSamples are stable for 12 months from date of receipt at -20℃ to -80℃. Upon delivery aliquot. Avoid freeze/thaw cycle.

Certificates

Certificate of Analysis(COA)

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3 results found

Lot NumberCertificate TypeDateItem
ZJ23F1101864Certificate of AnalysisDec 01, 2023 rp146397
ZJ23F1101863Certificate of AnalysisDec 01, 2023 rp146397
ZJ23F1101862Certificate of AnalysisDec 01, 2023 rp146397

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