| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| rp146523-5μg | 5μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $79.90 | |
| rp146523-10μg | 10μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $129.90 | |
| rp146523-20μg | 20μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $169.90 | |
| rp146523-100μg | 100μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $489.90 | |
| rp146523-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,779.90 |
Animal Free, >98% (SDS-PAGE, HPLC ), Active, E.coli, No tag, 18-144aa
| Product Name | Recombinant Human GM-CSF Protein, ＞98% (SDS-PAGE, HPLC ), high purity |
|---|---|
| Synonyms | Human Granulocyte-Macrophage Colony Stimulating Factor; Colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; CSF2; CSF-2; GMCSF; GM-CSF; GMCSFgranulocyte-macrophage colony-stimulating factor; granulocyte-macrophage colony |
| Grade | ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance |
| Product Description | Purity ＞98% (SDS-PAGE; HPLC) . Purity is greater than 98% as determined by SEC-HPLC and reducing SDS-PAGE. Function Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Human Granulocyte-Macrophage Colony Stimulating Factor Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) is secreted by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimulation. It was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors and has functions of stimulates the growth and differentiation of hematopoietic precursor cells from various lineages. GM-CSF has also been reported to have a functional role on non-hematopoietic cells and can induce human endothelial cells to migrate and proliferate. Additionally, it can stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. GM-CSF is used as a medication to stimulate the production of white blood cells following chemotherapy and has also recently been evaluated in clinical trials for its potential as a vaccine adjuvant in HIV-infected patients. The recombinant Human GM-CSF is a non-glycosylated polypeptide chain containing 127 amino acids. |
| Specifications & Purity | ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥98%(SDS-PAGE&HPLC) |
| Purity | ＞98% (SDS-PAGE, HPLC ) |
| Bioactivity | Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is typically < 0.2ng/mL. |
| Endotoxin Concentration | ＜0.01 EU/μg |
| Expression System | E.coli |
| Species | Human |
| Amino Acids | 18-144 aa |
| Sequence | APARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFD LQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSC ATQIITFESFKENLKDFLLVIPFDCWEPVQE |
| Protein Tag | No tag |
| Protein Length | Full length protein |
| Accession # | P04141 |
| Source | Recombinant |
| Predicted molecular weight | 14.5 kD |
| SDS-PAGE | 13.7 kDa, under reducing conditions; 12.3 kDa, under non-reducing conditions. |
Recombinant Human GM-CSF Protein (rp146523)-Protein Bioactivity
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED₅₀ for this effect is typically <0.2ng/mL.
Recombinant Human GM-CSF Protein (rp146523)-SDS-PAGE
3μg/lane of Recombinant Human GM-CSF Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 13.7 kDa.
| Form | Lyophilized |
|---|---|
| Reconstitution | 1.This vial must be briefly centrifuged prior to opening to bring the contents to the bottom。 2、 Reconstitute in a sterile aqueous buffer to an appropriate concentration。 Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. F |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Upon delivery aliquot, Store at -20°C, Avoid freeze / thaw cycles. Lyophilized with no additives. This product is an active protein and may elicit a biological response in vivo, handle with caution. |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0600490 | Certificate of Analysis | Jul 03, 2023 | rp146523 |
| ZJ23F0600489 | Certificate of Analysis | Jul 03, 2023 | rp146523 |
| ZJ23F0600488 | Certificate of Analysis | Jul 03, 2023 | rp146523 |
| ZJ23F0600487 | Certificate of Analysis | Jul 03, 2023 | rp146523 |
