Recombinant Human Granzyme B Protein

Features and benefits
  • Expression System: HEK293
  • Protein Tag: C-His
  • Bioactivity: Immobilized Recombinant Human Granzyme B Protein (rp181293) at 1.0 μg/mL can bind Granzyme B Antibody (Ab106347) with the EC50 of 1.923 ng/mL.
  • Endotoxin Concentration: <1.0 EU/μg
Item Number
rp181293
Grouped product items
SKUSizeAvailabilityPrice Qty
rp181293-10μg (Trial Size)
Apply for free trial size(?)
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10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
rp181293-50μg
50μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$199.90
rp181293-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
rp181293-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,599.90

Animal Free, ≥95% (SDS-PAGE), Active, 293F cell, C-His tag, 1-247 aa

Basic Description

Product NameRecombinant Human Granzyme B Protein
GradeActiBioPure™, Animal Free, Bioactive, Carrier Free
Product Description

Granzyme B, also known as GZMB, is the most prominent member of the granzyme family of cell death-inducing serine proteases expressed in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Granzyme B enters the target cells depending on another membrane-binding granule protein, perforin, results in the activation of effector caspases and mitochondrial depolarization through caspase-dependent and -independent pathways, and consequently induces rapid cell apoptosis. Over 3 substrates of GZMB have been identified including the key substrate caspase-3, ICAD, and Bid. GZMB is suggested to protect the host by lysing cells bearing on their surface 'nonself' antigens such as bacterial and viral infected-cells and tumor cells and accordingly plays an essential role in immunosurveillance.

Specifications & PurityActiBioPure™, Bioactive, Animal Free, Carrier Free, ≥95%(SDS-PAGE)
BioactivityImmobilized Recombinant Human Granzyme B Protein (rp181293) at 1.0 μg/mL can bind Granzyme B Antibody (Ab106347) with the EC50 of 1.923 ng/mL.
Endotoxin Concentration<1.0 EU/μg
Expression SystemHEK293
Amino Acids1-247 aa
SequenceMQPILLLLAFLLLPRADAGEIIGGHEAKPHSRPYMAYLMIWDQKSLKRCGGFLIRDDFVLTAAHCWGSSINVTLGAHNIKEQEPTQQFIPVKRPIPHPAYNPKNFSNDIMLLQLERKAKRTRAVQPLRLPSNKAQVKPGQTCSVAGWGQTAPLGKHSHTLQEVKMTVQEDRKCESDLRHYYDSTIELCVGDPEIKKTSFKGDSGGPLVCNKVAQGIVSYGRNNGMPPRACTKVSSFVHWIKKTMKRYHHHHHHHH
Protein TagC-His
Predicted molecular weight26.9 kDa
SDS-PAGE34.6 kDa, under reducing conditions; 34.6 kDa, under non-reducing conditions

Images

Recombinant Human Granzyme B Protein (rp181293) - ELISA
Immobilized Recombinant Human Granzyme B Protein (rp181293) at 1.0 μg/mL can bind Granzyme B Antibody (Ab106347) with the EC₅₀ of 1.923 ng/mL.

Recombinant Human Granzyme B Protein (rp181293) - SDS-PAGE
3 μg/lane of Recombinant Human Granzyme B Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 34.6 kDa.

Product Specifications

ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.1 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilu
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -20°C for 1 year. Avoid freeze / thaw cycle.

Certificates

Certificate of Analysis(COA)

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4 results found

Lot NumberCertificate TypeDateItem
ZJ24F0707704Certificate of AnalysisJul 05, 2024 rp181293
ZJ24F0707703Certificate of AnalysisJul 05, 2024 rp181293
ZJ24F0707702Certificate of AnalysisJul 05, 2024 rp181293
ZJ24R0600680Certificate of AnalysisJun 14, 2024 rp181293

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