Recombinant Human GRO Gamma Protein, >97%(SDS-PAGE and HPLC), high purity

Features and benefits
  • Expression System: E.coli
  • Accession #: P19876
  • Protein Tag: No tag
  • Bioactivity: Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected human 293 cells is in a concentration range of 10-100 ng/ml.
  • Endotoxin Concentration: <1.0 EU/μg
Item Number
rp146664
Grouped product items
SKUSizeAvailabilityPrice Qty
rp146664-10μg
10μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$139.90
rp146664-50μg
50μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$519.90
rp146664-100μg
100μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$769.90
rp146664-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,999.90

Animal Free, >97%(SDS-PAGE and HPLC), Active, E.coli, No tag, 35-107 aa

Basic Description

Product NameRecombinant Human GRO Gamma Protein, >97%(SDS-PAGE and HPLC), high purity
SynonymsC-X-C motif chemokine 3 | C-X-C motif chemokine ligand 3 | Chemokine (C X C motif) ligand 3 | Chemokine (CXC motif) ligand 3 | Cinc 2 | CINC 2b | Cinc2 | CINC2b | CXCL 3 | Cxcl3 | CXCL3_HUMAN | Cytokine induced neutrophil chemoattractant 2 | Dcip1 | Dendr
GradeActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance
Product Description

Purity
> 97% by SDS-PAGE and HPLC analyses.

Function
Ligand for CXCR2 (By similarity). Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes.

Post-translational
N-terminal processed form GRO-gamma(5-73) is produced by proteolytic cleavage after secretion from peripheral blood monocytes.

Specifications & PurityActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥97%(SDS-PAGE&HPLC)
Purity>97%(SDS-PAGE and HPLC)
BioactivityFully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected human 293 cells is in a concentration range of 10-100 ng/ml.
Endotoxin Concentration<1.0 EU/μg
Expression SystemE.coli
SpeciesHuman
Amino Acids35-107 aa
Protein TagNo tag
Accession #P19876
Predicted molecular weight7.9 kDa

Images

Recombinant Human GRO Gamma Protein (rp146664)-Protein Bioactivity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected human 293 cells is in a concentration range of 10-100 ng/mL.

Recombinant Human GRO Gamma Protein (rp146664)-SDS-PAGE
Recombinant Human GRO Gamma Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining. Showing a band at 7.9 kDa under reducing conditions and 10.6 kDa under non-reducing conditions.

Product Specifications

FormLyophilized
ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportio
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconst

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3 results found

Lot NumberCertificate TypeDateItem
ZJ23F0600469Certificate of AnalysisMar 26, 2024 rp146664
ZJ23F0600470Certificate of AnalysisMar 26, 2024 rp146664
ZJ23F0600471Certificate of AnalysisMar 26, 2024 rp146664

Related Documents

References

1. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P et al..  (2004)  The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)..  Genome Res,  14  (10B): (2121-7).  [PMID:15489334] [10.1021/op500134e]
2. Tekamp-Olson, P P and 7 more authors..  (1990)  Cloning and characterization of cDNAs for murine macrophage inflammatory protein 2 and its human homologues..  The Journal of experimental medicine,    (1):   [PMID:2201751]
3. Haskill, S S and 9 more authors..  (1990)  Identification of three related human GRO genes encoding cytokine functions..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:2217207]
4. Wuyts, A A and 7 more authors..  (1999)  Isolation of the CXC chemokines ENA-78, GRO alpha and GRO gamma from tumor cells and leukocytes reveals NH2-terminal heterogeneity. Functional comparison of different natural isoforms..  European journal of biochemistry,      [PMID:10095777]
5. Zhang, Zemin Z and Henzel, William J WJ..  (2004)  Signal peptide prediction based on analysis of experimentally verified cleavage sites..  Protein science : a publication of the Protein Society,      [PMID:15340161]
6. Hillier, Ladeana W LW and 121 more authors..  (2005)  Generation and annotation of the DNA sequences of human chromosomes 2 and 4..  Nature,    (7):   [PMID:15815621]

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