| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| rp175908-10μg (Trial Size) Apply for free trial size（?） Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free! | 10μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $139.90 | |
| rp175908-50μg | 50μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $359.90 | |
| rp175908-100μg | 100μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $559.90 | |
| rp175908-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
Animal Free, ≥95% (SDS-PAGE), Active, 293F cell, C-His tag, 1-728 aa (D384N, D416N, L622S, H645R, V678I)
| Product Name | Recombinant Human HGF Protein |
|---|---|
| Grade | ActiBioPure™, Animal Free, Bioactive, Carrier Free, High Performance |
| Specifications & Purity | Animal Free, Carrier Free, Bioactive, High performance, ActiBioPure™, ≥95%(SDS-PAGE) |
| Biochemical and Physiological Mechanisms | HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It's a multidomain molecule with an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain |
| Bioactivity | 1. Determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells. The ED50 for this effect is 24.77 ng/mL. 2. Immobilized Recombinant Human HGFR/c-MET Protein (rp169642) at 1.0 μg/mL can bind Biotinylated Recombinant Huma |
| Endotoxin Concentration | <1 EU/μg |
| Expression System | HEK293 |
| Amino Acids | 1-728 aa (D384N, D416N, L622S, H645R, V678I) |
| Sequence | MWVTKLLPALLLQHVLLHLLLLPIAIPYAEGQRKRRNTIHEFKKSAKTTLIKIDPALKIKTKKVNTADQCANRCTRNKGLPFTCKAFVFDKARKQCLWFPFNSMSSGVKKEFGHEFDLYENKDYIRNCIIGKGRSYKGTVSITKSGIKCQPWSSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGLMDHTESGKICQRWDHQTPHRHKFLPERYPDKGF |
| Protein Tag | C-His |
| Predicted molecular weight | 80.5 kDa |
| SDS-PAGE | 30.0-40.0 kDa & 56.0-65.0 kDa & 85.0-95.0 kDa, under reducing condition; 67.0-77.2 kDa, under non-reducing condition |
Recombinant Human HGF Protein (rp175908) - Protein Bioactivity
Determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells. The ED
₅₀
for this effect is 24.77 ng/mL.
Recombinant Human HGF Protein (rp175908) - Protein Bioactivity
Immobilized Recombinant Human HGFR/c-MET Protein (rp169642) at 1.0 μg/mL can bind Biotinylated Recombinant Human HGF Protein (rp175908) with the EC
₅₀
for this effect is 97.73 ng/mL.
Recombinant Human HGF Protein (rp175908) - ELISA
Immobilized Recombinant Human HGF Protein (rp156180) at 1.0 μg/mL can bind Ficlatuzumab (anti-HGF) (Ab170563) with the EC
₅₀
of 45.64 ng/mL.
Recombinant Human HGF Protein (rp175908) - ELISA
Immobilized Recombinant Human HGF Protein (rp156180) at 1.0 μg/mL can bind Rilotumumab (anti-HGF) (Ab170950) with the EC
₅₀
of 21.92 ng/mL.
Recombinant Human HGF Protein (rp175908) - SDS-PAGE
2 μg/lane of Recombinant Human HGF Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 30.0-40.0 kDa & 56.0-65.0 kDa & 85.0-95.0 kDa under reducing conditions and 67.0-77.2 kDa under non-reducing conditions.
| Form | Lyophilized |
|---|---|
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilu |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at -20°C stable up to 1 year. Avoid freeze / thaw cycle. |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0606558 | Certificate of Analysis | Jun 07, 2024 | rp175908 |
| ZJ24F0606557 | Certificate of Analysis | Jun 07, 2024 | rp175908 |
| ZJ24F0606556 | Certificate of Analysis | Jun 07, 2024 | rp175908 |
