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Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description: Histones are a complex family of highly conserved basic proteins responsible for packaging chromosomal DNA into nucleosomes. Histone proteins exhibit two levels of diversity: 1. evolutionary diversity between species and 2. subtype diversity in a class(H1, H2A, H2B, H3 or H4) within a species. It has become more and more evident that histone modifications are key players in the regulation of chromatin states and dynamics as well as in gene expression. Therefore, histone modifications and the enzymatic machinery that set them are crucial regulators that can control cellular proliferation, differentiation, plasticity, and malignancy processes. However, extracellular histones are a double-edged sword because they also damage host tissue and may cause death. Histones bound to platelets, induced calcium influx, and recruited plasma adhesion proteins such as fibrinogen to induce platelet aggregation. Histone H2B proteins have been studied in a variety of species and are easily detected in most species. The reversible ubiquitylation of histone H2B has long been implicated in transcriptional activation and gene silencing. Phosphorylation of H2B serine 32 occurs in normal cycling and mitogen-stimulated cells. Notably, this phosphorylation is elevated in skin cancer cell lines and tissues compared with normal counterparts. HIST2H2BE is a member of the histone H2B family and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
Recombinant Human Histone H2B Protein (rp156676) - SDS-PAGE 3 μg/lane of Recombinant Human Histone H2B Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 14.0 kDa.
Product Specifications
Form
Lyophilized
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilu
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20°C stable up to 1 year. Avoid freeze / thaw cycle.
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1.Albig, W W, Kardalinou, E E, Drabent, B B, Zimmer, A A and Doenecke, D D.. (1991) Isolation and characterization of two human H1 histone genes within clusters of core histone genes.. Genomics, [PMID:1916825]
2.Galasinski, Scott C SC, Louie, Donna F DF, Gloor, Kristen K KK, Resing, Katheryn A KA and Ahn, Natalie G NG.. (2002) Global regulation of post-translational modifications on core histones.. The Journal of biological chemistry, (25):[PMID:11709551]
3.Cheung, Wang L WL and 10 more authors.. (2003) Apoptotic phosphorylation of histone H2B is mediated by mammalian sterile twenty kinase.. Cell, (16):[PMID:12757711]
4.Golebiowski, Filip F and Kasprzak, Kazimierz S KS.. (2005) Inhibition of core histones acetylation by carcinogenic nickel(II).. Molecular and cellular biochemistry, [PMID:16283522]
5.Zhu, Bing B and 6 more authors.. (2005) Monoubiquitination of human histone H2B: the factors involved and their roles in HOX gene regulation.. Molecular cell, (23):[PMID:16307923]
6.Pavri, Rushad R and 6 more authors.. (2006) Histone H2B monoubiquitination functions cooperatively with FACT to regulate elongation by RNA polymerase II.. Cell, (19):[PMID:16713563]
7.Wu, Lipeng L, Zee, Barry M BM, Wang, Yanming Y, Garcia, Benjamin A BA and Dou, Yali Y.. (2011) The RING finger protein MSL2 in the MOF complex is an E3 ubiquitin ligase for H2B K34 and is involved in crosstalk with H3 K4 and K79 methylation.. Molecular cell, (8):[PMID:21726816]
8.Zhang, Zhuo Z and 13 more authors.. (2013) USP49 deubiquitinates histone H2B and regulates cotranscriptional pre-mRNA splicing.. Genes & development, (15):[PMID:23824326]
9.Goudarzi, Afsaneh and 22 more authors.. (2016) Dynamic Competing Histone H4 K5K8 Acetylation and Butyrylation Are Hallmarks of Highly Active Gene Promoters.. Molecular cell, (21):[PMID:27105113]
10.Xie, Zhongyu and 26 more authors.. (2016) Metabolic Regulation of Gene Expression by Histone Lysine β-Hydroxybutyrylation.. Molecular cell, (21):[PMID:27105115]
11.Grundy, Gabrielle J GJ and 12 more authors.. (2016) PARP3 is a sensor of nicked nucleosomes and monoribosylates histone H2B(Glu2).. Nature communications, (17):[PMID:27530147]
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