Recombinant Human Histone H3 Protein, >90% (SDS-PAGE), high purity

Features and benefits
  • Expression System: E. coli
  • Protein Tag: N-His
Item Number
rp156635
Grouped product items
SKUSizeAvailabilityPrice Qty
rp156635-10μg (Trial Size)
Apply for free trial size(?)
Every year, as a valued customer, you have the exclusive opportunity to explore and enjoy three different trial products of your choice, absolutely free!
10μg
In stock
$139.90
rp156635-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$499.90
rp156635-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$3,999.90

Carrier Free, >90% (SDS-PAGE), E.coli, N-His tag, 1-136 aa

Basic Description

Product NameRecombinant Human Histone H3 Protein, >90% (SDS-PAGE), high purity
SynonymsH3.5 Protein; Gm14384 Protein; OTTMUSG00000016509 Protein; fj58b09 Protein; wu:fj58b09 Protein; zgc:64222 Protein; H3 histone family member 3C Protein; H3 histone; family 3C Protein; H3F3C Protein; H3f3c Protein; h3f3c Protein; H3 histone family member E
GradeAzide Free, Carrier Free
Product Description

Purity:>90%, by SDS-PAGE visualized with Coomassie® Blue Staining.

Description:

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. H3 histone family member E pseudogene, H3 histone family, member A, H3/A, H31_HUMAN, H3F3, H3FA, Hist1h3a, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, HIST3H3, histone 1, H3a, Histone cluster 1, H3a

Specifications & PurityCarrier Free, Azide Free, ≥90%(SDS-PAGE)
Purity>90% (SDS-PAGE)
Expression SystemE. coli
Amino Acids1-136 aa
SequenceMGHHHHHHMARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEACEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA
Protein TagN-His
Predicted molecular weight15.4 kDa
SDS-PAGE16.2 kDa, reducing conditions; 16.2 kDa, non-reducing conditions

Images

Recombinant Human Histone H3 Protein (rp156635) - SDS-PAGE
Recombinant Human Histone H3 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 16.4 kDa under reducing conditions, showing two bands at 16.2 kDa and 32.0 kDa under non-reducing conditions .

Product Specifications

FormLyophilized
ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilu
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -20°C for 1 year. Avoid freeze / thaw cycle.

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ23F1101867Certificate of AnalysisDec 13, 2023 rp156635
ZJ23R1100164Certificate of AnalysisNov 24, 2023 rp156635

Related Documents

References

1. Ananthanarayanan M, Li S, Balasubramaniyan N, Suchy FJ, Walsh MJ.  (2004)  Ligand-dependent activation of the farnesoid X-receptor directs arginine methylation of histone H3 by CARM1..  J Biol Chem,  279  (52): (54348-57).  [PMID:15471871]
2. Marashi, F F and 6 more authors..  (1986)  Enhancer-facilitated expression of prokaryotic and eukaryotic genes using human histone gene 5' regulatory sequences..  Biochemistry and cell biology = Biochimie et biologie cellulaire,      [PMID:3013246]
3. Zhong, R R, Roeder, R G RG and Heintz, N N..  (1983)  The primary structure and expression of four cloned human histone genes..  Nucleic acids research,    (11):   [PMID:6647026]
4. Ohe, Y Y and Iwai, K K..  (1981)  Human spleen histone H3. Isolation and amino acid sequence..  Journal of biochemistry,      [PMID:7309716]
5. Goto, H H and 10 more authors..  (1999)  Identification of a novel phosphorylation site on histone H3 coupled with mitotic chromosome condensation..  The Journal of biological chemistry,    (3):   [PMID:10464286]
6. Lachner, M M, O'Carroll, D D, Rea, S S, Mechtler, K K and Jenuwein, T T..  (2001)  Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins..  Nature,    (1):   [PMID:11242053]
7. Preuss, Ute U, Landsberg, Gerd G and Scheidtmann, Karl Heinz KH..  (2003)  Novel mitosis-specific phosphorylation of histone H3 at Thr11 mediated by Dlk/ZIP kinase..  Nucleic acids research,    (1):   [PMID:12560483]
8. Huyen, Yentram Y and 9 more authors..  (2004)  Methylated lysine 79 of histone H3 targets 53BP1 to DNA double-strand breaks..  Nature,    (18):   [PMID:15525939]
9. Dai, Jun J, Sultan, Sammy S, Taylor, Stephen S SS and Higgins, Jonathan M G JM..  (2005)  The kinase haspin is required for mitotic histone H3 Thr 3 phosphorylation and normal metaphase chromosome alignment..  Genes & development,    (15):   [PMID:15681610]
10. Choi, Hong Seok HS and 5 more authors..  (2005)  Phosphorylation of Ser28 in histone H3 mediated by mixed lineage kinase-like mitogen-activated protein triple kinase alpha..  The Journal of biological chemistry,    (8):   [PMID:15684425]
11. Macdonald, Neil N and 12 more authors..  (2005)  Molecular basis for the recognition of phosphorylated and phosphoacetylated histone h3 by 14-3-3..  Molecular cell,    (28):   [PMID:16246723]
12. Hake, Sandra B SB and 8 more authors..  (2006)  Expression patterns and post-translational modifications associated with mammalian histone H3 variants..  The Journal of biological chemistry,    (6):   [PMID:16267050]
13. Flanagan, John F JF and 8 more authors..  (2005)  Double chromodomains cooperate to recognize the methylated histone H3 tail..  Nature,    (22):   [PMID:16372014]
14. Thomas, C Eric CE, Kelleher, Neil L NL and Mizzen, Craig A CA..  (2006)  Mass spectrometric characterization of human histone H3: a bird's eye view..  Journal of proteome research,      [PMID:16457588]
15. Miao, Feng F, Li, ShuLian S, Chavez, Valerie V, Lanting, Linda L and Natarajan, Rama R..  (2006)  Coactivator-associated arginine methyltransferase-1 enhances nuclear factor-kappaB-mediated gene transcription through methylation of histone H3 at arginine 17..  Molecular endocrinology (Baltimore, Md.),      [PMID:16497732]
16. Beck, Hans Christian HC and 8 more authors..  (2006)  Quantitative proteomic analysis of post-translational modifications of human histones..  Molecular & cellular proteomics : MCP,      [PMID:16627869]
17. Morris, Stephanie A SA and 9 more authors..  (2007)  Identification of histone H3 lysine 36 acetylation as a highly conserved histone modification..  The Journal of biological chemistry,    (9):   [PMID:17189264]
18. Garcia, Benjamin A BA and 10 more authors..  (2007)  Organismal differences in post-translational modifications in histones H3 and H4..  The Journal of biological chemistry,    (9):   [PMID:17194708]
19. Guccione, Ernesto E and 7 more authors..  (2007)  Methylation of histone H3R2 by PRMT6 and H3K4 by an MLL complex are mutually exclusive..  Nature,    (18):   [PMID:17898714]
20. Metzger, Eric E and 11 more authors..  (2008)  Phosphorylation of histone H3 at threonine 11 establishes a novel chromatin mark for transcriptional regulation..  Nature cell biology,      [PMID:18066052]
21. Iberg, Aimee N AN and 6 more authors..  (2008)  Arginine methylation of the histone H3 tail impedes effector binding..  The Journal of biological chemistry,    (8):   [PMID:18077460]
22. Hyllus, Dawin D and 7 more authors..  (2007)  PRMT6-mediated methylation of R2 in histone H3 antagonizes H3 K4 trimethylation..  Genes & development,    (15):   [PMID:18079182]
23. Shimada, Midori M and 6 more authors..  (2008)  Chk1 is a histone H3 threonine 11 kinase that regulates DNA damage-induced transcriptional repression..  Cell,    (25):   [PMID:18243098]
24. Metzger, Eric E and 14 more authors..  (2010)  Phosphorylation of histone H3T6 by PKCbeta(I) controls demethylation at histone H3K4..  Nature,    (1):   [PMID:20228790]
25. Vermeulen, Michiel M and 10 more authors..  (2010)  Quantitative interaction proteomics and genome-wide profiling of epigenetic histone marks and their readers..  Cell,    (17):   [PMID:20850016]
26. Wu, Gang and 21 more authors..  (2012)  Somatic histone H3 alterations in pediatric diffuse intrinsic pontine gliomas and non-brainstem glioblastomas..  Nature genetics,    (29):   [PMID:22286216]
27. Yu, Yongxin Y and 7 more authors..  (2012)  Histone H3 lysine 56 methylation regulates DNA replication through its interaction with PCNA..  Molecular cell,    (13):   [PMID:22387026]
28. Yang, Weiwei W and 9 more authors..  (2012)  PKM2 phosphorylates histone H3 and promotes gene transcription and tumorigenesis..  Cell,    (17):   [PMID:22901803]
29. Tropberger, Philipp P and 11 more authors..  (2013)  Regulation of transcription through acetylation of H3K122 on the lateral surface of the histone octamer..  Cell,    (14):   [PMID:23415232]
30. Chan, Kui-Ming KM and 11 more authors..  (2013)  The histone H3.3K27M mutation in pediatric glioma reprograms H3K27 methylation and gene expression..  Genes & development,    (1):   [PMID:23603901]
31. Lu, Chao C and 29 more authors..  (2016)  Histone H3K36 mutations promote sarcomagenesis through altered histone methylation landscape..  Science (New York, N.Y.),    (13):   [PMID:27174990]
32. Li, Lei L and 14 more authors..  (2016)  SIRT7 is a histone desuccinylase that functionally links to chromatin compaction and genome stability..  Nature communications,    (20):   [PMID:27436229]
33. Herranz, Nicolás N and 12 more authors..  (2016)  Lysyl oxidase-like 2 (LOXL2) oxidizes trimethylated lysine 4 in histone H3..  The FEBS journal,      [PMID:27735137]
34. Papillon-Cavanagh, Simon S and 22 more authors..  (2017)  Impaired H3K36 methylation defines a subset of head and neck squamous cell carcinomas..  Nature genetics,      [PMID:28067913]
35. Wang, Yugang Y and 17 more authors..  (2017)  KAT2A coupled with the α-KGDH complex acts as a histone H3 succinyltransferase..  Nature,    (14):   [PMID:29211711]
36. Palazzo, Luca L and 5 more authors..  (2018)  Serine is the major residue for ADP-ribosylation upon DNA damage..  eLife,    (26):   [PMID:29480802]
37. Kardalinou, E E, Eick, S S, Albig, W W and Doenecke, D D..  (1993)  Association of a human H1 histone gene with an H2A pseudogene and genes encoding H2B.1 and H3.1 histones..  Journal of cellular biochemistry,      [PMID:8227173]

Solution Calculators