Recombinant Human Hsp60 Protein, >95% (SDS-PAGE), high purity

Features and benefits
  • Expression System: E. coli
  • Protein Tag: N-His
Item Number
rp169616
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rp169616-10μg (Trial Size)
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10μg
In stock
$139.90
rp169616-100μg
100μg
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$599.90
rp169616-1mg
1mg
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$2,599.90

Carrier Free, >95% (SDS-PAGE), E.coli, His tag, 1-573 aa

Basic Description

Product NameRecombinant Human Hsp60 Protein, >95% (SDS-PAGE), high purity
Synonyms60 kDa chaperonin | Chaperonin 60 | CPN60 | EC 5.6.1.7 | Heat shock protein 60 | Hsp60 | HSP-60 | HuCHA60 | Mitochondrial matrix protein P1 | P60 lymphocyte protein | 60 kDa chaperonin | Chaperonin 60 (CPN60) | Heat shock protein 60 (HSP-60 | Hsp60) | HuC
GradeAzide Free, Carrier Free
Product Description

Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining.
Description:
HSPD1, also known as HSP60, is a member of the chaperonin family. HSPD1 may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. It may also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. HSPD1 gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13). Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4); also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. HSPD1 is clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurs within the first two decades of life.

Specifications & PurityCarrier Free, Azide Free, ≥95%(SDS-PAGE)
Purity>95% (SDS-PAGE)
Expression SystemE. coli
Amino Acids1-573 aa
SequenceMGSSHHHHHHSSGLVPRGSHMLRLPTVFRQMRPVSRVLAPHLTRAYAKDVKFGADARALMLQGVDLLADAVAVTMGPKGRTVIIEQSWGSPKVTKDGVTVAKSIDLKDKYKNIGAKLVQDVANNTNEEAGDGTTTATVLARSIAKEGFEKISKGANPVEIRRGVMLAVDAVIAELKKQSKPVTTPEEIAQVATISANGDKEIGNIISDAMKKVGRKGVITVKDGKTLNDELEIIEGMKFDRGYISPYFINTSKGQ
Protein TagN-His
Predicted molecular weight63.2 kDa
SDS-PAGE62.0 kDa, under reducing conditions.

Images

Recombinant Human Hsp60 Protein (rp169616) - SDS-PAGE
3 μg/lane of Recombinant Human Hsp60 Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining, showing a band at 62.0 kDa under reducing conditions.

Product Specifications

FormLyophilized
ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilu
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -20°C for 1 year, store at 2-8℃ for 1 month. Avoid freeze / thaw cycle.

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3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0102578Certificate of AnalysisJan 30, 2024 rp169616
ZJ24F0102577Certificate of AnalysisJan 30, 2024 rp169616
ZJ24F0102576Certificate of AnalysisJan 30, 2024 rp169616

Related Documents

References

1. Nagumo Y, Kakeya H, Yamaguchi J, Uno T, Shoji M, Hayashi Y, Osada H..  (2004)  Structure-activity relationships of epolactaene derivatives: structural requirements for inhibition of Hsp60 chaperone activity..  Bioorg Med Chem Lett,  14  (17): (4425-4429).  [PMID:15357965]
2. Wijeratne EM, Gunaherath GM, Chapla VM, Tillotson J, de la Cruz F, Kang M, U'Ren JM, Araujo AR, Arnold AE, Chapman E, Gunatilaka AA..  (2016)  Oxaspirol B with p97 Inhibitory Activity and Other Oxaspirols from Lecythophora sp. FL1375 and FL1031, Endolichenic Fungi Inhabiting Parmotrema tinctorum and Cladonia evansii..  J Nat Prod,  79  (2): (340-352).  [PMID:26812276]
3. Abdeen S, Salim N, Mammadova N, Summers CM, Goldsmith-Pestana K, McMahon-Pratt D, Schultz PG, Horwich AL, Chapman E, Johnson SM..  (2016)  Targeting the HSP60/10 chaperonin systems of Trypanosoma brucei as a strategy for treating African sleeping sickness..  Bioorg Med Chem Lett,  26  (21): (5247-5253).  [PMID:27720295]
4. Tillotson J, Kedzior M, Guimarães L, Ross AB, Peters TL, Ambrose AJ, Schmidlin CJ, Zhang DD, Costa-Lotufo LV, Rodríguez AD, Schatz JH, Chapman E..  (2017)  ATP-competitive, marine derived natural products that target the DEAD box helicase, eIF4A..  Bioorg Med Chem Lett,  27  (17): (4082-4085).  [PMID:28757063]
5. Abdeen S, Kunkle T, Salim N, Ray AM, Mammadova N, Summers C, Stevens M, Ambrose AJ, Park Y, Schultz PG, Horwich AL, Hoang QQ, Chapman E, Johnson SM..  (2018)  Sulfonamido-2-arylbenzoxazole GroEL/ES Inhibitors as Potent Antibacterials against Methicillin-Resistant Staphylococcus aureus (MRSA)..  J Med Chem,  61  (16): (7345-7357).  [PMID:30060666]
6. Kunkle T, Abdeen S, Salim N, Ray AM, Stevens M, Ambrose AJ, Victorino J, Park Y, Hoang QQ, Chapman E, Johnson SM..  (2018)  Hydroxybiphenylamide GroEL/ES Inhibitors Are Potent Antibacterials against Planktonic and Biofilm Forms of Staphylococcus aureus..  J Med Chem,  61  (23): (10651-10664).  [PMID:30392371]
7. Washburn A, Abdeen S, Ovechkina Y, Ray AM, Stevens M, Chitre S, Sivinski J, Park Y, Johnson J, Hoang QQ, Chapman E, Parish T, Johnson SM..  (2019)  Dual-targeting GroEL/ES chaperonin and protein tyrosine phosphatase B (PtpB) inhibitors: A polypharmacology strategy for treating Mycobacterium tuberculosis infections..  Bioorg Med Chem Lett,  29  (13): (1665-1672).  [PMID:31047750]
8. Stevens M, Abdeen S, Salim N, Ray AM, Washburn A, Chitre S, Sivinski J, Park Y, Hoang QQ, Chapman E, Johnson SM..  (2019)  HSP60/10 chaperonin systems are inhibited by a variety of approved drugs, natural products, and known bioactive molecules..  Bioorg Med Chem Lett,  29  (9): (1106-1112).  [PMID:30852084]
9. Hochstrasser, D F DF and 9 more authors..  (1992)  Human liver protein map: a reference database established by microsequencing and gel comparison..  Electrophoresis,      [PMID:1286669]
10. Singh, B B, Patel, H V HV, Ridley, R G RG, Freeman, K B KB and Gupta, R S RS..  (1990)  Mitochondrial import of the human chaperonin (HSP60) protein..  Biochemical and biophysical research communications,    (15):   [PMID:1972619]
11. Venner, T J TJ, Singh, B B and Gupta, R S RS..  (1990)  Nucleotide sequences and novel structural features of human and Chinese hamster hsp60 (chaperonin) gene families..  DNA and cell biology,      [PMID:1980192]
12. Ward, L D LD, Hong, J J, Whitehead, R H RH and Simpson, R J RJ..  (1990)  Development of a database of amino acid sequences for human colon carcinoma proteins separated by two-dimensional polyacrylamide gel electrophoresis..  Electrophoresis,      [PMID:2079031]
13. Jindal, S S, Dudani, A K AK, Singh, B B, Harley, C B CB and Gupta, R S RS..  (1989)  Primary structure of a human mitochondrial protein homologous to the bacterial and plant chaperonins and to the 65-kilodalton mycobacterial antigen..  Molecular and cellular biology,      [PMID:2568584]
14. Waldinger, D D, Eckerskorn, C C, Lottspeich, F F and Cleve, H H..  (1988)  Amino-acid sequence homology of a polymorphic cellular protein from human lymphocytes and the chaperonins from Escherichia coli (groEL) and chloroplasts (Rubisco-binding protein)..  Biological chemistry Hoppe-Seyler,      [PMID:2907406]
15. Corbett, J M JM, Wheeler, C H CH, Baker, C S CS, Yacoub, M H MH and Dunn, M J MJ..  (1994)  The human myocardial two-dimensional gel protein database: update 1994..  Electrophoresis,      [PMID:7895732]
16. Rasmussen, R K RK and 6 more authors..  ()  Two-dimensional electrophoretic analysis of human breast carcinoma proteins: mapping of proteins that bind to the SH3 domain of mixed lineage kinase MLK2..  Electrophoresis,      [PMID:9150946]
17. Ji, H H and 5 more authors..  ()  A two-dimensional gel database of human colon carcinoma proteins..  Electrophoresis,      [PMID:9150948]
18. Hansen, Jens Jacob JJ and 10 more authors..  (2002)  Hereditary spastic paraplegia SPG13 is associated with a mutation in the gene encoding the mitochondrial chaperonin Hsp60..  American journal of human genetics,      [PMID:11898127]
19. Hansen, Jens J JJ and 9 more authors..  (2003)  Genomic structure of the human mitochondrial chaperonin genes: HSP60 and HSP10 are localised head to head on chromosome 2 separated by a bidirectional promoter..  Human genetics,      [PMID:12483302]
20. Gevaert, Kris K and 6 more authors..  (2003)  Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides..  Nature biotechnology,      [PMID:12665801]
21. She, Yi-Min YM and 5 more authors..  (2003)  Identification of metal-binding proteins in human hepatoma lines by immobilized metal affinity chromatography and mass spectrometry..  Molecular & cellular proteomics : MCP,      [PMID:14534351]
22. Andersen, Jens S JS and 5 more authors..  (2003)  Proteomic characterization of the human centrosome by protein correlation profiling..  Nature,    (4):   [PMID:14654843]
23. Tanaka, Yasuo Y and 11 more authors..  (2004)  Interaction of the hepatitis B virus X protein (HBx) with heat shock protein 60 enhances HBx-mediated apoptosis..  Biochemical and biophysical research communications,    (28):   [PMID:15120623]
24. Aboulaich, Nabila N, Vainonen, Julia P JP, Strålfors, Peter P and Vener, Alexander V AV..  (2004)  Vectorial proteomics reveal targeting, phosphorylation and specific fragmentation of polymerase I and transcript release factor (PTRF) at the surface of caveolae in human adipocytes..  The Biochemical journal,    (15):   [PMID:15242332]
25. Beausoleil, Sean A SA, Villén, Judit J, Gerber, Scott A SA, Rush, John J and Gygi, Steven P SP..  (2006)  A probability-based approach for high-throughput protein phosphorylation analysis and site localization..  Nature biotechnology,      [PMID:16964243]
26. Magen, Daniella D and 13 more authors..  (2008)  Mitochondrial hsp60 chaperonopathy causes an autosomal-recessive neurodegenerative disorder linked to brain hypomyelination and leukodystrophy..  American journal of human genetics,      [PMID:18571143]
27. Peng, Chao C and 18 more authors..  (2011)  The first identification of lysine malonylation substrates and its regulatory enzyme..  Molecular & cellular proteomics : MCP,      [PMID:21908771]
28. Merle, Nicolas N and 8 more authors..  (2012)  ATAD3B is a human embryonic stem cell specific mitochondrial protein, re-expressed in cancer cells, that functions as dominant negative for the ubiquitous ATAD3A..  Mitochondrion,      [PMID:22664726]
29. Cloutier, Philippe P, Lavallée-Adam, Mathieu M, Faubert, Denis D, Blanchette, Mathieu M and Coulombe, Benoit B..  (2013)  A newly uncovered group of distantly related lysine methyltransferases preferentially interact with molecular chaperones to regulate their activity..  PLoS genetics,      [PMID:23349634]
30. Dihanich, Sybille S and 6 more authors..  (2014)  GTP binding controls complex formation by the human ROCO protein MASL1..  The FEBS journal,      [PMID:24286120]
31. Nisemblat, Shahar S, Yaniv, Oren O, Parnas, Avital A, Frolow, Felix F and Azem, Abdussalam A..  (2015)  Crystal structure of the human mitochondrial chaperonin symmetrical football complex..  Proceedings of the National Academy of Sciences of the United States of America,    (12):   [PMID:25918392]

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