| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| rp147252-100μg | 100μg | In stock | $79.90 | |
| rp147252-1mg | 1mg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $239.90 | |
| rp147252-500μg | 500μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $179.90 |
Animal Free, ≥95% (SDS-PAGE), Active, E.coli, No tag, 49-188 aa
| Product Name | Recombinant Human IGF-1 Protein, ＞95% SDS-PAGE, high purity |
|---|---|
| Synonyms | IBP1; IGF1; IGF-1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Soma |
| Grade | ActiBioPure™, Animal Free, Bioactive, Carrier Free, High performance |
| Product Description | The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. |
| Specifications & Purity | Animal Free, Carrier Free, Bioactive, ActiBioPure™, High performance, ≥95%(SDS-PAGE) |
| Purity | ＞95% SDS-PAGE |
| Bioactivity | Measured in a cell proliferation assay using MCF7 breast cancer cell line. The ED₅₀ for this effect is typically <4.6 ng/mL. |
| Endotoxin Concentration | <0.2 EU/µg |
| Expression System | Pichia pastoris |
| Species | Human |
| Amino Acids | 49-118 aa |
| Sequence | GP ETLCGAELVD ALQFVCGDRG FYFNKPTGYG SSSRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPAKSA |
| Protein Tag | No tag |
| N-terminal Sequence | Gly |
| Protein Length | Full length protein |
| Accession # | P05019 |
| Source | Recombinant |
| Predicted molecular weight | 7.7 kDa |
| SDS-PAGE | 8.5 kDa, under reducing conditions; 8.5 kDa, under non-reducing conditions. |
Recombinant Human IGF-1 Protein (rp147252)-Protein Bioactivity
Measured in a cell proliferation assay using MCF7 breast cancer cell line. The ED₅₀ for this effect is typically <4.6ng/mL.
Recombinant Human IGF-1 Protein (rp147252)-SDS-PAGE
5μg/lane of Recombinant Human IGF-1 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 8.5 kDa.
| Form | Lyophilized |
|---|---|
| Reconstitution | Reconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Stable at -20℃ to 24 months, stable at 2-8 ℃ to 4 weeks. Prepared as 100μg/ml storage solution, 2~8℃ can be stably stored for a week. Upon delivery aliquot. Avoid freeze/thaw cycle. |
Enter Lot Number to search for COA:
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| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0102336 | Certificate of Analysis | Jan 30, 2024 | rp147252 |
| ZJ23F0400100 | Certificate of Analysis | Apr 06, 2023 | rp147252 |
| ZJ23F0400101 | Certificate of Analysis | Apr 06, 2023 | rp147252 |
| 1. Rinderknecht, E E and Humbel, R E RE.. (1978) The amino acid sequence of human insulin-like growth factor I and its structural homology with proinsulin.. The Journal of biological chemistry, (25): [PMID:632300] |
| 2. Sato, A A and 7 more authors.. (1992) 1H-NMR assignment and secondary structure of human insulin-like growth factor-I (IGF-I) in solution.. Journal of biochemistry, [PMID:1319992] |
| 3. Sandberg-Nordqvist, A C AC, Ståhlbom, P A PA, Lake, M M and Sara, V R VR.. (1992) Characterization of two cDNAs encoding insulin-like growth factor 1 (IGF-1) in the human fetal brain.. Brain research. Molecular brain research, [PMID:1372070] |
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| 8. Rotwein, P P, Pollock, K M KM, Didier, D K DK and Krivi, G G GG.. (1986) Organization and sequence of the human insulin-like growth factor I gene. Alternative RNA processing produces two insulin-like growth factor I precursor peptides.. The Journal of biological chemistry, (15): [PMID:2937782] |
| 9. de Pagter-Holthuizen, P P and 6 more authors.. (1986) Organization of the human genes for insulin-like growth factors I and II.. FEBS letters, (20): [PMID:3002851] |
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| 11. and Rotwein, P P.. (1986) Two insulin-like growth factor I messenger RNAs are expressed in human liver.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:3455760] |
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| 13. Jansen, M M and 8 more authors.. (1983) Sequence of cDNA encoding human insulin-like growth factor I precursor.. Nature, [PMID:6358902] |
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| 15. Sandberg-Nordqvist, A C AC and 5 more authors.. (1993) Characterization of insulin-like growth factor 1 in human primary brain tumors.. Cancer research, (1): [PMID:8495408] |
| 16. Woods, K A KA, Camacho-Hübner, C C, Savage, M O MO and Clark, A J AJ.. (1996) Intrauterine growth retardation and postnatal growth failure associated with deletion of the insulin-like growth factor I gene.. The New England journal of medicine, (31): [PMID:8857020] |
| 17. Cargill, M M and 17 more authors.. (1999) Characterization of single-nucleotide polymorphisms in coding regions of human genes.. Nature genetics, [PMID:10391209] |
| 18. Scherer, Steven E SE and 216 more authors.. (2006) The finished DNA sequence of human chromosome 12.. Nature, (16): [PMID:16541075] |
| 19. Saegusa, Jun J and 7 more authors.. (2009) The direct binding of insulin-like growth factor-1 (IGF-1) to integrin alphavbeta3 is involved in IGF-1 signaling.. The Journal of biological chemistry, (4): [PMID:19578119] |
| 20. Wang, Lei L and 5 more authors.. (2010) The SRC homology 2 domain protein Shep1 plays an important role in the penetration of olfactory sensory axons into the forebrain.. The Journal of neuroscience : the official journal of the Society for Neuroscience, (29): [PMID:20881139] |
| 21. Zoidis, E E, Ghirlanda-Keller, C C and Schmid, C C.. (2011) Stimulation of glucose transport in osteoblastic cells by parathyroid hormone and insulin-like growth factor I.. Molecular and cellular biochemistry, [PMID:21076856] |
| 22. Fujita, Masaaki M and 7 more authors.. (2012) Cross-talk between integrin α6β4 and insulin-like growth factor-1 receptor (IGF1R) through direct α6β4 binding to IGF1 and subsequent α6β4-IGF1-IGF1R ternary complex formation in anchorage-independent conditions.. The Journal of biological chemistry, (6): [PMID:22351760] |
| 23. Fujita, Masaaki M, Takada, Yoko K YK and Takada, Yoshikazu Y.. (2013) Insulin-like growth factor (IGF) signaling requires αvβ3-IGF1-IGF type 1 receptor (IGF1R) ternary complex formation in anchorage independence, and the complex formation does not require IGF1R and Src activation.. The Journal of biological chemistry, (1): [PMID:23243309] |
| 24. Fujita, Masaaki M and 13 more authors.. (2013) An integrin binding-defective mutant of insulin-like growth factor-1 (R36E/R37E IGF1) acts as a dominant-negative antagonist of the IGF1 receptor (IGF1R) and suppresses tumorigenesis but still binds to IGF1R.. The Journal of biological chemistry, (5): [PMID:23696648] |
| 25. Shcheglovitov, Aleksandr A and 10 more authors.. (2013) SHANK3 and IGF1 restore synaptic deficits in neurons from 22q13 deletion syndrome patients.. Nature, (14): [PMID:24132240] |
| 26. Shaheen, Ranad R and 8 more authors.. (2014) Genomic analysis of primordial dwarfism reveals novel disease genes.. Genome research, [PMID:24389050] |
