| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| rp147287-10μg | 10μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $89.90 | |
| rp147287-50μg | 50μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $249.90 | |
| rp147287-100μg | 100μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $399.90 | |
| rp147287-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,999.90 |
Animal Free, >96%(SDS-PAGE and HPLC), Active, E.coli, No tag, 21-272 aa
| Product Name | Recombinant Human IGFBP5 Protein, ＞96%(SDS-PAGE and HPLC), high purityused for Cell Culture,WB,Active,SDS-PAGE various applications |
|---|---|
| Synonyms | IBP5IBP-5 | IGF-binding protein 5 | IGFBP5 | IGFBP-5 | Insulin-like growth factor binding protein 5 | Insulin-like growth factor-binding protein 5 | IBP 5 | IBP-5 | IBP5 | IBP5_HUMAN | IGF binding protein 5 | IGF BP5 | IBP-5 | IGF-binding protein 5 | IGFB |
| Grade | ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance |
| Product Description | Purity |
| Specifications & Purity | ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥96%(SDS-PAGE&HPLC) |
| Purity | ＞96%(SDS-PAGE and HPLC) |
| Bioactivity | Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit IGF-II induced proliferation of MCF-7 cells is less than 0.4 μg/ml, corresponding to a specific activity of > 2500 IU/mg in the presence of 15 ng/ml of r |
| Endotoxin Concentration | <0.1 EU/μg |
| Expression System | E. coli |
| Species | Human |
| Amino Acids | 21-272aa |
| Sequence | LGSFVHCEPCDEKALSMCPPSPLGCELVKEPGCGCCMTCALAEGQSCGVYTERCAQGLRCLPRQDEEKPLHALLHGRGVCLNEKSYREQVKIERDSREHEEPTTSEMAEETYSPKIFRPKHTRISELKAEAVKKDRRKKLTQSKFVGGAENTAHPRIISAPEMRQESEQGPCRRHMEASLQELKASPRMVPRAVYLPNCDRKGFYKRKQCKPSRGRKRGICWCVDKYGMKLPGMEYVDGDFQCHTFDSSNVE |
| Protein Tag | No tag |
| Protein Length | Full length protein |
| Accession # | P24593 |
| Source | Recombinant |
| Predicted molecular weight | 28.6 kDa |
| SDS-PAGE | 28.6 kDa |
Recombinant Human IGFBP5 Protein (rp147287)-Protein Bioactivity
Fully biologically active when compared to standard. The ED₅₀ as determined by its ability to inhibit IGF-II induced proliferation of MCF-7 cells is less than 0.4 μg/mL, corresponding to a specific activity of > 2500 IU/mg in the presence of 15 ng/mL of rHuIGF-II.
Recombinant Human IGFBP5 Protein (rp147287)-SDS-PAGE
Recombinant Human IGFBP5 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 28.6 kDa.
| Application | Cell Culture,WB,Active,SDS-PAGE |
|---|---|
| Form | Lyophilized |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportio |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Upon delivery aliquot, Store at -20°C, Avoid freeze/thaw cycles. Lyophilized with no additives. This product is an active protein and may elicit a biological response in vivo, handle with caution. |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0600314 | Certificate of Analysis | Mar 26, 2024 | rp147287 |
| ZJ23F0600315 | Certificate of Analysis | Mar 26, 2024 | rp147287 |
| ZJ23F0600316 | Certificate of Analysis | Mar 26, 2024 | rp147287 |
| 1. Shimasaki, S S, Shimonaka, M M, Zhang, H P HP and Ling, N N.. (1991) Identification of five different insulin-like growth factor binding proteins (IGFBPs) from adult rat serum and molecular cloning of a novel IGFBP-5 in rat and human.. The Journal of biological chemistry, (5): [PMID:1709938] |
| 2. Andress, D L DL and Birnbaum, R S RS.. (1991) A novel human insulin-like growth factor binding protein secreted by osteoblast-like cells.. Biochemical and biophysical research communications, (15): [PMID:1850257] |
| 3. Kiefer, M C MC, Ioh, R S RS, Bauer, D M DM and Zapf, J J.. (1991) Molecular cloning of a new human insulin-like growth factor binding protein.. Biochemical and biophysical research communications, (15): [PMID:1850258] |
| 4. Allander, S V SV and 9 more authors.. (1994) Characterization of the chromosomal gene and promoter for human insulin-like growth factor binding protein-5.. The Journal of biological chemistry, (8): [PMID:7511611] |
| 5. Kalus, W W and 10 more authors.. (1998) Structure of the IGF-binding domain of the insulin-like growth factor-binding protein-5 (IGFBP-5): implications for IGF and IGF-I receptor interactions.. The EMBO journal, (16): [PMID:9822601] |
| 6. Ständker, L L, Wobst, P P, Mark, S S and Forssmann, W G WG.. (1998) Isolation and characterization of circulating 13-kDa C-terminal fragments of human insulin-like growth factor binding protein-5.. FEBS letters, (18): [PMID:9883900] |
| 7. Tagliabracci, Vincent S VS and 14 more authors.. (2015) A Single Kinase Generates the Majority of the Secreted Phosphoproteome.. Cell, (18): [PMID:26091039] |
