ActiBioPure™, Animal Free, Bioactive, Carrier Free, High Performance
Product Description
Function Directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production.
Specifications & Purity
Animal Free, Carrier Free, Bioactive, ActiBioPure™, High performance, ≥96%(SDS-PAGE&HPLC)
Purity
>96% (SDS-PAGE,HPLC)
Bioactivity
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED₅₀ for this effect is typically <2ng/mL.
Recombinant Human IL-11 Protein (rp147371)-Protein Bioactivity Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED₅₀ for this effect is typically <2ng/mL.
Recombinant Human IL-11 Protein (rp147371)-SDS-PAGE 3μg/lane of Recombinant Human IL-11 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 18.3 kDa.
Product Specifications
Form
Lyophilized
Reconstitution
This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile aqueous buffer to an appropriate concentration. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further
Storage Temp
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
For long term storage, the product should be stored ≤ -20℃. 36 months, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -70℃ under sterile conditions after reconstitution. Upon delivery aliquot. Avo
Certificates
Certificate of Analysis(COA)
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1.Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K et al.. (2004) Complete sequencing and characterization of 21,243 full-length human cDNAs.. Nat Genet, 36 (1):(40-5). [PMID:14702039][10.1021/op500134e]
2.McKinley, D D, Wu, Q Q, Yang-Feng, T T and Yang, Y C YC.. (1992) Genomic sequence and chromosomal location of human interleukin-11 gene (IL11).. Genomics, [PMID:1386338]
3.Kawashima, I I and 7 more authors.. (1991) Molecular cloning of cDNA encoding adipogenesis inhibitory factor and identity with interleukin-11.. FEBS letters, (3):[PMID:1828438]
4.Paul, S R SR and 9 more authors.. (1990) Molecular cloning of a cDNA encoding interleukin 11, a stromal cell-derived lymphopoietic and hematopoietic cytokine.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2145578]
5.Harmegnies, Dimitri D and 9 more authors.. (2003) Characterization of a potent human interleukin-11 agonist.. The Biochemical journal, (1):[PMID:12919066]
6.Grimwood, Jane J and 97 more authors.. (2004) The DNA sequence and biology of human chromosome 19.. Nature, (1):[PMID:15057824]
7.Putoczki, Tracy L TL, Dobson, Renwick C J RC and Griffin, Michael D W MD.. (2014) The structure of human interleukin-11 reveals receptor-binding site features and structural differences from interleukin-6.. Acta crystallographica. Section D, Biological crystallography, [PMID:25195742]
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