Recombinant Human IL-15 Protein, >97% (SDS-PAGE), high purity

Features and benefits
  • Expression System: E.coli
  • Accession #: P40933
  • Protein Tag: No tag
  • Bioactivity: The ED₅₀ as determined by the dose-dependent stimulation of the proliferation of CTLL-2 cells was found to be ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2 x 10⁶ units/mg.
  • Endotoxin Concentration: <0.01 EU/μg
Item Number
rp147404
Grouped product items
SKUSizeAvailabilityPrice Qty
rp147404-10μg
10μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$139.90
rp147404-50μg
50μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$599.90
rp147404-100μg
100μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$899.90
rp147404-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$3,299.90

Animal Free, >97% (SDS-PAGE), Active, E. coli., No tag, 49-162 aa

Basic Description

Product NameRecombinant Human IL-15 Protein, >97% (SDS-PAGE), high purity
SynonymsIL15; IL-15; IL-15MGC9721; interleukin 15; interleukin-15
GradeActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance
Product Description

Purity

>97% SDS-PAGE.


Function

Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha.

Specifications & PurityActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥97%(SDS-PAGE)
Purity>97% (SDS-PAGE)
BioactivityThe ED₅₀ as determined by the dose-dependent stimulation of the proliferation of CTLL-2 cells was found to be ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2 x 10⁶ units/mg.
Endotoxin Concentration<0.01 EU/μg
Expression SystemE.coli
SpeciesHuman
Amino Acids49-162 aa
SequenceNWVNVISDLKKIEDLIQSMHIDATLYTESDVHPSCKVTAMKCFLLELQVISLESGDASIHDTVENLIILANNSLSSNGNVTESGCKECEELEEKNIKEFLQSFVHIVQMFINTS
Protein TagNo tag
Accession #P40933
Predicted molecular weight12.9 kDa
SDS-PAGE10.8 kDa, under reducing conditions; 10.8 kDa, under non-reducing conditions.

Images

Recombinant Human IL-15 Protein (rp147404) - Protein Bioactivity
Recombinant Human IL-15 stimulates cell proliferation of the CTLL-2 mouse cytotoxic T cell line. The ED₅₀ for this effect is typically less than 0.5 ng/mL.

Recombinant Human IL-15 Protein (rp147404) - SDS-PAGE
3 μg/lane of Recombinant Human IL-15 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 10.8 kDa.

Product Specifications

FormLyophilized
ReconstitutionReconstitution Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageFor long term storage, the product should be stored ≤-20℃. Please avoid repeated freeze-thaw cycles after reconstitution. 12 months from date of receipt, -20 to -70℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -2

Certificates

Certificate of Analysis(COA)

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3 results found

Lot NumberCertificate TypeDateItem
ZJ23F0600493Certificate of AnalysisJul 03, 2023 rp147404
ZJ23F0600492Certificate of AnalysisJul 03, 2023 rp147404
ZJ23F0600491Certificate of AnalysisJul 03, 2023 rp147404

Related Documents

References

1. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K et al..  (2004)  Complete sequencing and characterization of 21,243 full-length human cDNAs..  Nat Genet,  36  (1): (40-5).  [PMID:14702039]
2. Quéméner A, Maillasson M, Arzel L, Sicard B, Vomiandry R, Mortier E, Dubreuil D, Jacques Y, Lebreton J, Mathé-Allainmat M.  (2017)  Discovery of a Small-Molecule Inhibitor of Interleukin 15: Pharmacophore-Based Virtual Screening and Hit Optimization..  J Med Chem,  60  (14): (6249-6272).  [PMID:28657314]
3. Żyżyńska-Granica B, Trzaskowski B, Niewieczerzał S, Filipek S, Zegrocka-Stendel O, Dutkiewicz M, Krzeczyński P, Kowalewska M, Koziak K..  (2017)  Pharmacophore guided discovery of small-molecule interleukin 15 inhibitors..  Eur J Med Chem,  136  (543-547).  [PMID:28535470]
4. Johnston, J A JA and 9 more authors..  (1995)  Tyrosine phosphorylation and activation of STAT5, STAT3, and Janus kinases by interleukins 2 and 15..  Proceedings of the National Academy of Sciences of the United States of America,    (12):   [PMID:7568001]
5. Giri, J G JG and 9 more authors..  (1994)  Utilization of the beta and gamma chains of the IL-2 receptor by the novel cytokine IL-15..  The EMBO journal,    (15):   [PMID:8026467]
6. Grabstein, K H KH and 9 more authors..  (1994)  Cloning of a T cell growth factor that interacts with the beta chain of the interleukin-2 receptor..  Science (New York, N.Y.),    (13):   [PMID:8178155]
7. Meazza, R R and 8 more authors..  (1996)  Identification of a novel interleukin-15 (IL-15) transcript isoform generated by alternative splicing in human small cell lung cancer cell lines..  Oncogene,    (16):   [PMID:8668345]
8. Krause, H H and 5 more authors..  (1996)  Genomic structure and chromosomal localization of the human interleukin 15 gene (IL-15)..  Cytokine,      [PMID:8932977]
9. Badolato, R R, Ponzi, A N AN, Millesimo, M M, Notarangelo, L D LD and Musso, T T..  (1997)  Interleukin-15 (IL-15) induces IL-8 and monocyte chemotactic protein 1 production in human monocytes..  Blood,    (1):   [PMID:9326248]
10. Tagaya, Y Y and 7 more authors..  (1997)  Generation of secretable and nonsecretable interleukin 15 isoforms through alternate usage of signal peptides..  Proceedings of the National Academy of Sciences of the United States of America,    (23):   [PMID:9405632]
11. Ratthé, Claude C and Girard, Denis D..  (2004)  Interleukin-15 enhances human neutrophil phagocytosis by a Syk-dependent mechanism: importance of the IL-15Ralpha chain..  Journal of leukocyte biology,      [PMID:15123770]
12. Ring, Aaron M AM and 12 more authors..  (2012)  Mechanistic and structural insight into the functional dichotomy between IL-2 and IL-15..  Nature immunology,      [PMID:23104097]

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