| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| rp147444-10μg | 10μg | In stock | $849.90 | |
| rp147444-50μg | 50μg | In stock | $2,369.90 | |
| rp147444-100μg | 100μg | In stock | $3,789.90 | |
| rp147444-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $24,589.90 |
Animal Free, >95% (SDS-PAGE&SEC-HPLC), Active, E.coli, No tag, 37-193 aa
| Product Name | Recombinant Human IL-18 Protein, >95% (SDS-PAGE&SEC-HPLC), high purity |
|---|---|
| Synonyms | Iboctadekin; IFN-gamma-inducing factor; IGIF; IGIFIL-1 gamma; IL18; IL-18; IL-18MGC12320; IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; interleukin-18; IFN gamma |
| Grade | ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance |
| Product Description | Purity |
| Specifications & Purity | ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE&SEC-HPLC) |
| Purity | >95% (SDS-PAGE&SEC-HPLC) |
| Bioactivity | 1、Measured by its ability to induce IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED50 for this effect is typically 5 - 30 ng/mL. 2、Recombinant Human IL-18 Protein (rp147444) stimulates cell prolifera |
| Endotoxin Concentration | ＜10 EU/mg |
| Expression System | E.coli |
| Species | Human |
| Amino Acids | 37-193 aa |
| Sequence | YFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED |
| Protein Tag | No tag |
| Accession # | Q14116 |
| Predicted molecular weight | 18.2 kDa |
| SDS-PAGE | 15.4 kDa, under reducing conditions; 15.4 kDa, under non-reducing conditions. |
Recombinant Human IL-18 Protein (rp147444) - Protein Bioactivity
Measured by its ability to induce IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED₅₀ for this effect is typically 5 - 30 ng/mL.
Recombinant Human IL-18 Protein (rp147444) - Protein Bioactivity
Recombinant Human IL-18 Protein (rp147444) stimulates cell proliferation of the CTLL-2 mouse cytotoxic T cell line. The ED₅₀ for this effect is typically 0.27 ng/mL.
Recombinant Human IL-18 Protein (rp147444) - SDS-PAGE
3 μg/lane of Recombinant Human IL-18 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 15.4 kDa.
| Form | Lyophilized |
|---|---|
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25mg/mL. |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Samples are stable for twelve months from date of receipt at -20℃ to -80℃. Upon delivery aliquot. Avoid freeze/thaw cycle. |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1202262 | Certificate of Analysis | Dec 28, 2023 | rp147444 |
| ZJ23F1202261 | Certificate of Analysis | Dec 28, 2023 | rp147444 |
| ZJ23F1202260 | Certificate of Analysis | Dec 28, 2023 | rp147444 |
| 1. Todd D Taylor,Hideki Noguchi,Yasushi Totoki,Atsushi Toyoda,Yoko Kuroki,Ken Dewar,Christine Lloyd,Takehiko Itoh,Tadayuki Takeda,Dae-Won Kim,Xinwei She,Karen F Barlow,Toby Bloom,Elspeth Bruford,Jean L Chang,Christina A Cuomo,Evan Eichler,Michael G FitzGerald,David B Jaffe,Kurt LaButti,Robert Nicol,Hong-Seog Park,Christopher Seaman,Carrie Sougnez,Xiaoping Yang,Andrew R Zimmer,Michael C Zody,Bruce W Birren,Chad Nusbaum,Asao Fujiyama,Masahira Hattori,Jane Rogers,Eric S Lander,Yoshiyuki Sakaki. (2006-03-24) Human chromosome 11 DNA sequence and analysis including novel gene identification.. Nature, 440 ((7083)): (497-500). [PMID:16554811] |
| 2. Ushio, S S and 15 more authors.. (1996) Cloning of the cDNA for human IFN-gamma-inducing factor, expression in Escherichia coli, and studies on the biologic activities of the protein.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:8666798] |
| 3. Tominaga, K K and 7 more authors.. (2000) IL-12 synergizes with IL-18 or IL-1beta for IFN-gamma production from human T cells.. International immunology, [PMID:10653850] |
| 4. Kato, Zenichiro Z and 14 more authors.. (2003) The structure and binding mode of interleukin-18.. Nature structural biology, [PMID:14528293] |
| 5. Gaggero, Alessia A and 7 more authors.. (2004) A novel isoform of pro-interleukin-18 expressed in ovarian tumors is resistant to caspase-1 and -4 processing.. Oncogene, (30): [PMID:15326478] |
| 6. Jourdan, Tony T and 13 more authors.. (2013) Activation of the Nlrp3 inflammasome in infiltrating macrophages by endocannabinoids mediates beta cell loss in type 2 diabetes.. Nature medicine, [PMID:23955712] |
| 7. Wei, Hui H and 6 more authors.. (2014) Structural basis for the specific recognition of IL-18 by its alpha receptor.. FEBS letters, (3): [PMID:25261253] |
| 8. Tsutsumi, Naotaka N and 12 more authors.. (2014) The structural basis for receptor recognition of human interleukin-18.. Nature communications, (15): [PMID:25500532] |
