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Recombinant Human IL-4 Protein, >98% (SDS-PAGE; HPLC ), high purity

  • ActiBioPure™
  • Animal Free
  • Azide Free
  • Bioactive
  • Carrier Free
  • High Performance
  • ≥95%(SDS-PAGE)
Features and benefits
  • Expression System: HEK293
  • Accession #: P05112
  • Protein Tag: C-His
  • Bioactivity: Measured in a cell proliferation assay using TF 1 human erythroleukemic cells. The ED50 for this effect is 85-340 pg/mL, corresponding to a specific activity of 2.94 × 106~1.17× 107 units/mg.
  • Endotoxin Concentration: <0.1 EU/µg
Item Number
rp147593
Grouped product items
SKUSizeAvailabilityPrice Qty
rp147593-50μg
50μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
rp147593-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$499.90
rp147593-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,799.90

Animal Free, ≥95% (SDS-PAGE), Active, 293F, C-His tag, 25-153 aa

View related series
Accession#:P05112 Gene ID:3565 IL4

Basic Description

Product NameRecombinant Human IL-4 Protein, >98% (SDS-PAGE; HPLC ), high purity
SynonymsB cell growth factor 1|BCDF|B-cell stimulatory factor 1|BCGF1|BCGF-1|binetrakin|BSF1|BSF-1|IL4|IL-4|IL-4B_cell stimulatory factor 1|IL4E12|interleukin 4|interleukin-4|Lymphocyte stimulatory factor 1|MGC79402|Pitrakinra|B-cell stimulatory factor 1|Binetrak
GradeActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance
Product Description

Purity
≥95% (SDS-PAGE)
Endotoxin level
<0.1 EU/μg
Function
Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 (IL-4) is a pleiotropic cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells and produced primarily by mast cells, Th2 cells, eosinophils and basophils. IL-4 is a key regulator in humoral and adaptive immunity and has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of B cells into plasma cells. It regulates the differentiation of naive CD4+ T cells into helper Th2 cells and immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells is associated with allergies. IL-4 has a compact, globular fold, stabilized by 3 disulfide bonds. The recombinant Interleukin-4 is a 15kDa protein consisting of 129amino acid residues.

Specifications & PurityActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE)
Purity>98% (SDS-PAGE; HPLC )
BioactivityMeasured in a cell proliferation assay using TF 1 human erythroleukemic cells. The ED50 for this effect is 85-340 pg/mL, corresponding to a specific activity of 2.94 × 106~1.17× 107 units/mg.
Endotoxin Concentration<0.1 EU/µg
Expression SystemHEK293
SpeciesHuman
Amino Acids25-153 aa
SequenceHKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSSHHHHHH
Protein TagC-His
Accession #P05112
Predicted molecular weight15 kDa
SDS-PAGE20-23 kDa, under reducing conditions

Images

Recombinant Human IL-4 Protein (rp147593) - Protein Bioactivity
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED₅₀ for this effect is 169.3 pg/mL.

Recombinant Human IL-4 Protein (rp147593) - SDS-PAGE
3 μg/lane of Recombinant Human IL-4 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 20.0 kDa.

Product Specifications

FormLyophilized
ReconstitutionReconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -20~-80℃ for more than 1 year. Upon delivery aliquot. Avoid freeze/thaw cycle.

Certificates

Certificate of Analysis(COA)

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3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0607351Certificate of AnalysisJun 28, 2024 rp147593
ZJ24F0607350Certificate of AnalysisJun 28, 2024 rp147593
ZJ24F0607349Certificate of AnalysisJun 28, 2024 rp147593

Related Documents

References

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Solution Calculators