Recombinant Human IL-6R Protein, >90% (SDS-PAGE), high purity

Features and benefits
  • Expression System: HEK293
  • Accession #: NP_000556.1, P08887
  • Protein Tag: C-His
  • Bioactivity: 1. Immobilized Recombinant Human IL-6 Protein at 2 μg/mL can bind Recombinant Human IL-6R Protein (rp147628) the EC50 is 8.0 - 24.0 ng/mL. 2. Measured by its ability to enhance the IL-6 activity on M1 mouse myeloid leukemia cells. The ED50 for this effect
  • Endotoxin Concentration: <1.0 EU/μg
Item Number
rp147628
Grouped product items
SKUSizeAvailabilityPrice Qty
rp147628-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$389.90
rp147628-50μg
50μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,429.90
rp147628-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,289.90
rp147628-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$16,499.90

Animal Free, >90% (SDS-PAGE), Active, HEK293, C-His tag, 20-365 aa

Basic Description

Product NameRecombinant Human IL-6R Protein, >90% (SDS-PAGE), high purity
SynonymsCD126 | gp80 | IL-6 R alpha | IL6Q | IL6R alpha | IL-6R alpha | IL6R | IL-6R-1 | IL6RA | IL-6Ra | IL6RQ | interleukin 6 receptor | HIES5 | IL-6R | IL-1Ra | IL6QTL
GradeActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance
Product Description

Function:
Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.
Low concentration of a soluble form of IL6 receptor acts as an agonist of IL6 activity.


Specifications & PurityActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥90%(SDS-PAGE)
Purity>90% (SDS-PAGE)
Bioactivity1. Immobilized Recombinant Human IL-6 Protein at 2 μg/mL can bind Recombinant Human IL-6R Protein (rp147628) the EC50 is 8.0 - 24.0 ng/mL. 2. Measured by its ability to enhance the IL-6 activity on M1 mouse myeloid leukemia cells. The ED50 for this effect
Endotoxin Concentration<1.0 EU/μg
Expression SystemHEK293
SpeciesHuman
Amino Acids20-365 aa
Protein TagC-His
Accession #NP_000556.1, P08887
Predicted molecular weight40 kDa
SDS-PAGE71.1 kDa, under reducing conditions; 68.4 kDa, under non-reducing conditions.

Images

Recombinant Human IL-6R Protein (rp147628) - Protein Bioactivity
Immobilized Recombinant Human IL-6 Protein at 2 μg/mL can bind Recombinant Human IL-6R Protein (rp147628) the EC₅₀ is 8.0 - 24.0 ng/mL.

Recombinant Human IL-6R Protein (rp147628) - Protein Bioactivity
Measured by its ability to enhance the IL-6 activity on M1 mouse myeloid leukemia cells. The ED₅₀ for this effect is typically 10.0 - 60.0 ng/mL.

Recombinant Human IL-6R Protein (rp147628) - BLI Assay
Using the Octet RED System, the affinity constant (Kd) of Recombinant Human IL-6R Protein (rp147628) bound Anti-IL6R Antibody was 0.1 nM.

Recombinant Human IL-6R Protein (rp147628) - SDS-PAGE
3 μg/lane of Recombinant Human IL-6R Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 71.1 kDa under reducing conditions and 68.4 kDa under non-reducing conditions.

Product Specifications

FormLyophilized
ReconstitutionIt is recommended that sterile water be added to the vial to prepare a stock solution of 0.25mg/mL.
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageSamples are stable for twelve months from date of receipt at -20℃ to -80℃. Upon delivery aliquot. Avoid freeze/thaw cycle.

Certificates

Certificate of Analysis(COA)

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3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0505980Certificate of AnalysisMay 24, 2024 rp147628
ZJ24F0505981Certificate of AnalysisMay 24, 2024 rp147628
ZJ24F0505982Certificate of AnalysisMay 24, 2024 rp147628

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