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Recombinant Human Kallikrein 6 Protein

≥95%(SDS-PAGE)

Features and benefits

Expression System: HEK293
Accession #: Q92876
Protein Tag: C-10His
Bioactivity: Measured by its ability to cleave the fluorogenic peptide substrate Boc-QAR-AMC. The specific activity is >1300 pmol/min/µg, as measured under the described conditions.
Endotoxin Concentration: <0.1 EU/μg

Item Number

rp147935

Grouped product items
SKU	Size	Availability	Price
rp147935-10μg	10μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$259.90
rp147935-50μg	50μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$779.90
rp147935-100μg	100μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$1,169.90
rp147935-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$5,849.90

Animal Free, ≥95% (SDS-PAGE), Active, 293F, C-10*His tag, 17-244 aa

View related series

Accession#: Q92876 Gene ID: 5653 Kallikrein 6 KLK6

Basic Description

Product Name	Recombinant Human Kallikrein 6 Protein
Synonyms	EC 3.4.21.- \| Neurosin \| Protease M \| Serine protease 18 \| Serine protease 9 \| SP59 \| Zyme \| Bssp \| EC 3.4.21 \| EC 3.4.21.- \| hK6 \| kallikrein 6 (neurosin, zyme) \| Kallikrein 6 \| kallikrein-6 \| kallikrein-related peptidase 6 \| KLK6 \| Klk7 \| Neurosin \| Pro
Grade	ActiBioPure™, Animal Free, Bioactive, Carrier Free
Product Description	Function Serine protease which exhibits a preference for Arg over Lys in the substrate P1 position and for Ser or Pro in the P2 position. Shows activity against amyloid precursor protein, myelin basic protein, gelatin, casein and extracellular matrix proteins such as fibronectin, laminin, vitronectin and collagen. Degrades alpha-synuclein and prevents its polymerization, indicating that it may be involved in the pathogenesis of Parkinson disease and other synucleinopathies. May be involved in regulation of axon outgrowth following spinal cord injury. Tumor cells treated with a neutralizing KLK6 antibody migrate less than control cells, suggesting a role in invasion and metastasis.
Specifications & Purity	Animal Free, Carrier Free, Bioactive, ActiBioPure™, ≥95%(SDS-PAGE)
Bioactivity	Measured by its ability to cleave the fluorogenic peptide substrate Boc-QAR-AMC. The specific activity is >1300 pmol/min/µg, as measured under the described conditions.
Endotoxin Concentration	<0.1 EU/μg
Expression System	HEK293
Species	Human
Amino Acids	17-244 aa
Sequence	EEQNKLVHGGPCDKTSHPYQAALYTSGHLLCGGVLIHPLWVLTAAHCKKPNLQVFLGKHNLRQRESSQEQSSVVRAVIHPDYDAASHDQDIMLLRLARPAKLSELIQPLPLERDCSANTTSCHILGWGKTADGDFPDTIQCAYIHLVSREECEHAYPGQITQNMLCAGDEKYGKDSCQGDSGGPLVCGDHLRGLVSWGNIPCGSKEKPGVYTNVCRYTNWIQKTIQAKHHHHHHHHHH
Protein Tag	C-10His
Accession #	Q92876
Predicted molecular weight	26 kDa
SDS-PAGE	35 kDa under reducing conditions.

Form

Liquid

Reconstitution

Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.

Storage Temp

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20~-80℃ for more than 1 year. Upon delivery aliquot. Avoid freeze/thaw cycle.

References

1. De Vita E, Schüler P, Lovell S, Lohbeck J, Kullmann S, Rabinovich E, Sananes A, Heßling B, Hamon V, Papo N et al.. (2018) Depsipeptides Featuring a Neutral P1 Are Potent Inhibitors of Kallikrein-Related Peptidase 6 with On-Target Cellular Activity.. J Med Chem, 61 (19): (8859-8874). [PMID:30212625] [10.1021/op500134e]
2. De Vita E, Smits N, van den Hurk H, Beck EM, Hewitt J, Baillie G, Russell E, Pannifer A, Hamon V, Morrison A et al.. (2020) Synthesis and Structure-Activity Relationships of N-(4-Benzamidino)-Oxazolidinones: Potent and Selective Inhibitors of Kallikrein-Related Peptidase 6.. ChemMedChem, 15 (1): (79-95). [PMID:31675166] [10.1021/op500134e]
3. Oliveira JP, Freitas RF, Melo LS, Barros TG, Santos JA, Juliano MA, Pinheiro S, Blaber M, Juliano L, Muri EM, Puzer L.. (2014) Isomannide-based peptidomimetics as inhibitors for human tissue kallikreins 5 and 7.. ACS Med Chem Lett, 5 (2): (128-132). [PMID:24900785] [10.1021/ml4003698]
4. Santos JA, Kondo MY, Freitas RF, dos Santos MH, Ramalho TC, Assis DM, Juliano L, Juliano MA, Puzer L.. (2016) The natural flavone fukugetin as a mixed-type inhibitor for human tissue kallikreins.. Bioorg Med Chem Lett, 26 (5): (1485-1489). [PMID:26848109] [10.1016/j.bmcl.2016.01.039]
5. Hoelz LV, Zorzanelli BC, Azevedo PH, Passos SG, de Souza LR, Zani M, Pinheiro S, Puzer L, Dias LR, Muri EM.. (2016) Synthesis, biological evaluation and molecular modeling of pseudo-peptides based statine as inhibitors for human tissue kallikrein 5.. Eur J Med Chem, 112 (39-47). [PMID:26874743] [10.1016/j.ejmech.2016.01.060]
6. Anisowicz, A A, Sotiropoulou, G G, Stenman, G G, Mok, S C SC and Sager, R R.. (1996) A novel protease homolog differentially expressed in breast and ovarian cancer.. Molecular medicine (Cambridge, Mass.), [PMID:8898378]
7. Yamashiro, K K and 7 more authors.. (1997) Molecular cloning of a novel trypsin-like serine protease (neurosin) preferentially expressed in brain.. Biochimica et biophysica acta, (3): [PMID:9003450]
8. Little, S P SP and 16 more authors.. (1997) Zyme, a novel and potentially amyloidogenic enzyme cDNA isolated from Alzheimer's disease brain.. The Journal of biological chemistry, (3): [PMID:9312124]
9. Yousef, G M GM, Luo, L Y LY, Scherer, S W SW, Sotiropoulou, G G and Diamandis, E P EP.. (1999) Molecular characterization of zyme/protease M/neurosin (PRSS9), a hormonally regulated kallikrein-like serine protease.. Genomics, (1): [PMID:10610719]
10. Ogawa, K K and 11 more authors.. (2000) Localization of a novel type trypsin-like serine protease, neurosin, in brain tissues of Alzheimer's disease and Parkinson's disease.. Psychiatry and clinical neurosciences, [PMID:10997858]
11. Diamandis, E P EP and 6 more authors.. (2000) Immunofluorometric assay of human kallikrein 6 (zyme/protease M/neurosin) and preliminary clinical applications.. Clinical biochemistry, [PMID:11018688]
12. Gan, L L and 8 more authors.. (2000) Sequencing and expression analysis of the serine protease gene cluster located in chromosome 19q13 region.. Gene, (17): [PMID:11054574]
13. Petraki, C D CD and 6 more authors.. (2001) The spectrum of human kallikrein 6 (zyme/protease M/neurosin) expression in human tissues as assessed by immunohistochemistry.. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, [PMID:11668196]
14. Bernett, Matthew J MJ and 5 more authors.. (2002) Crystal structure and biochemical characterization of human kallikrein 6 reveals that a trypsin-like kallikrein is expressed in the central nervous system.. The Journal of biological chemistry, (5): [PMID:11983703]
15. Gomis-Rüth, F Xavier FX and 7 more authors.. (2002) The structure of human prokallikrein 6 reveals a novel activation mechanism for the kallikrein family.. The Journal of biological chemistry, (26): [PMID:12016211]
16. Magklara, Angeliki A and 6 more authors.. (2003) Characterization of the enzymatic activity of human kallikrein 6: Autoactivation, substrate specificity, and regulation by inhibitors.. Biochemical and biophysical research communications, (8): [PMID:12878203]
17. Iwata, Atsushi A and 6 more authors.. (2003) Alpha-synuclein degradation by serine protease neurosin: implication for pathogenesis of synucleinopathies.. Human molecular genetics, (15): [PMID:12928483]
18. Pampalakis, Georgios G, Kurlender, Lisa L, Diamandis, Eleftherios P EP and Sotiropoulou, Georgia G.. (2004) Cloning and characterization of novel isoforms of the human kallikrein 6 gene.. Biochemical and biophysical research communications, (16): [PMID:15207701]
19. Ghosh, Manik C MC, Grass, Linda L, Soosaipillai, Antoninus A, Sotiropoulou, Georgia G and Diamandis, Eleftherios P EP.. () Human kallikrein 6 degrades extracellular matrix proteins and may enhance the metastatic potential of tumour cells.. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, [PMID:15557757]
20. Angelo, Pedro Francisco PF and 7 more authors.. (2006) Substrate specificity of human kallikrein 6: salt and glycosaminoglycan activation effects.. The Journal of biological chemistry, (10): [PMID:16321973]
21. Pampalakis, Georgios G and Sotiropoulou, Georgia G.. (2006) Multiple mechanisms underlie the aberrant expression of the human kallikrein 6 gene in breast cancer.. Biological chemistry, [PMID:16800739]
22. Blaber, Sachiko I SI, Yoon, Hyesook H, Scarisbrick, Isobel A IA, Juliano, Maria Aparecida MA and Blaber, Michael M.. (2007) The autolytic regulation of human kallikrein-related peptidase 6.. Biochemistry, (1): [PMID:17417874]

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Recombinant Human Kallikrein 6 Protein

Basic Description

Product Specifications

Certificates

Certificate of Analysis(COA)

Related Documents

References

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