ActiBioPure™, Azide Free, Bioactive, Carrier Free, GMP, High Performance
Product Description
Keratinocyte growth factor (KGF) is a cytokine found by Rubin et al. (1989) from the culture supernatant of embryonic lung fibroblasts, which is a member of the FGF family, namely FGF-7. KGF is an effective epithelial-specific growth factor secreted by mesenchymal cells and distributed in epithelial cells. Its mitotic activity is mainly manifested in keratinocytes, which can specifically promote the proliferation, migration and differentiation of epithelial cells. It is closely related to organ development, wound repair, tumor genesis and immune reconstruction. Activity definition: The ED50 value is less than 1.0 ng/ml, that is, the corresponding activity unit is greater than or equal to 1 x 10*6 units/mg, as determined by the proliferation method of cultured MCF-7 cells.
Specifications & Purity
ActiBioPure™, Bioactive, GMP, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE)
Bioactivity
Measured in a cell proliferation assay using MCF7 breast cancer cell line. The ED₅₀ for this effect is typically <10 ng/mL.
Endotoxin Concentration
<0.1 EU/μg
Expression System
Oryza sativa
Species
Human
Amino Acids
32-194 aa
Protein Tag
No tag
Source
Rice seed
Predicted molecular weight
19.0 kD
Images
Recombinant Human KGF Protein (rp155960)-Protein Bioactivity Measured in a cell proliferation assay using MCF7 breast cancer cell line. The ED₅₀ for this effect is typically <10ng/mL.
Recombinant Human KGF Protein (rp155960)-SDS-PAGE 2μg/lane of Recombinant Human KGF was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 16kDa. Another band is the HSA protein.
Product Specifications
Form
Lyophilized
Reconstitution
It is recommended to re dissolve OsrhKGF freeze-dried powder to 100-200 ug/ml with sterile water, and further dilute it with other solvents. The dissolved OsrhKGF can be stored for 2-7 days at 4 ℃ and used up as soon as possible. If it is not used for a s
Storage Temp
Store at -20°C
Shipped In
Ice chest + Ice pads
Stability And Storage
Shipped at 4°C. Store at +4°C short term (2-7 days). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Resolution: It is recommended to redissolve lyophilized powder with sterile water to 100-200 ug/ml, and further dilute it with other so
CAS
148348-15-6
Certificates
Certificate of Analysis(COA)
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