Recombinant Human LIF Protein, ≥95% (SDS-PAGE), high purity

Features and benefits
  • Expression System: HEK293
  • Accession #: P15018
  • Protein Tag: C-His
  • Bioactivity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.1-0.3 ng/mL.
  • Endotoxin Concentration: <0.1 EU/μg
Item Number
rp148270
Grouped product items
SKUSizeAvailabilityPrice Qty
rp148270-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$149.90
rp148270-50μg
50μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$439.90
rp148270-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$719.90
rp148270-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,879.90

Animal Free, ≥95% (SDS-PAGE), Active, 293F, C-His tag, 23-202 aa

Basic Description

Product NameRecombinant Human LIF Protein, ≥95% (SDS-PAGE), high purity
SynonymsCDF; D Factor; DIA; differentiation inhibitory activity; differentiation stimulating factor; Differentiation-stimulating factor; Emfilermin; HILDA; HILDAcholinergic differentiation factor; leukemia inhibitory factor (cholinergic differentiation factor); l
GradeActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance
Product Description

Purity
≥95% (SDS-PAGE)
Endotoxin level
<0.1 EU/μg
Function
LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.

Specifications & PurityActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE)
Purity≥95% (SDS-PAGE)
BioactivityMeasured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.1-0.3 ng/mL.
Endotoxin Concentration<0.1 EU/μg
Expression SystemHEK293
SpeciesHuman
Amino Acids23-202 aa
SequenceSPLPITPVNATCAIRHPCHNNLMNQIRSQLAQLNGSANALFILYYTAQGEPFPNNLDKLCGPNVTDFPPFHANGTEKAKLVELYRIVVYLGTSLGNITRDQKILNPSALSLHSKLNATADILRGLLSNVLCRLCSKYHVGHVDVTYGPDTSGKDVFQKKKLGCQLLGKYKQIIAVLAQAFHHHHHH
Protein TagC-His
Accession #P15018
Predicted molecular weight20 kDa
SDS-PAGE33-45 kDa, under reducing conditions

Images

Recombinant Human LIF Protein (rp148270) - SDS-PAGE
3 μg/lane of Recombinant Human LIF Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 35 - 45 kDa.

Product Specifications

FormLyophilized
ReconstitutionReconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -20~-80℃ for more than 1 year. Upon delivery aliquot. Avoid freeze/thaw cycle.

Certificates

Certificate of Analysis(COA)

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3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0506247Certificate of AnalysisMay 31, 2024 rp148270
ZJ24F0506246Certificate of AnalysisMay 31, 2024 rp148270
ZJ24F0506245Certificate of AnalysisMay 31, 2024 rp148270

Related Documents

References

1. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K et al..  (2004)  Complete sequencing and characterization of 21,243 full-length human cDNAs..  Nat Genet,  36  (1): (40-5).  [PMID:14702039]
2. Stahl, J J and 5 more authors..  (1990)  Structural organization of the genes for murine and human leukemia inhibitory factor. Evolutionary conservation of coding and non-coding regions..  The Journal of biological chemistry,    (25):   [PMID:1692837]
3. Lowe, D G DG and 9 more authors..  (1989)  Genomic cloning and heterologous expression of human differentiation-stimulating factor..  DNA (Mary Ann Liebert, Inc.),      [PMID:2475312]
4. Mori, M M, Yamaguchi, K K and Abe, K K..  (1989)  Purification of a lipoprotein lipase-inhibiting protein produced by a melanoma cell line associated with cancer cachexia..  Biochemical and biophysical research communications,    (15):   [PMID:2730639]
5. Gough, N M NM and 6 more authors..  (1988)  Molecular cloning and expression of the human homologue of the murine gene encoding myeloid leukemia-inhibitory factor..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:3128791]
6. Moreau, J F JF and 5 more authors..  (1988)  Leukaemia inhibitory factor is identical to the myeloid growth factor human interleukin for DA cells..  Nature,    (15):   [PMID:3143918]
7. Schmelzer, C H CH and 5 more authors..  (1993)  Glycosylation pattern and disulfide assignments of recombinant human differentiation-stimulating factor..  Archives of biochemistry and biophysics,      [PMID:8489250]
8. Dunham, I I and 24 more authors..  (1999)  The DNA sequence of human chromosome 22..  Nature,    (2):   [PMID:10591208]
9. Boulanger, Martin J MJ, Bankovich, Alexander J AJ, Kortemme, Tanja T, Baker, David D and Garcia, K Christopher KC..  (2003)  Convergent mechanisms for recognition of divergent cytokines by the shared signaling receptor gp130..  Molecular cell,      [PMID:14527405]
10. Brandenberger, Ralph R and 13 more authors..  (2004)  Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation..  Nature biotechnology,      [PMID:15146197]
11. Huyton, Trevor T and 6 more authors..  (2007)  An unusual cytokine:Ig-domain interaction revealed in the crystal structure of leukemia inhibitory factor (LIF) in complex with the LIF receptor..  Proceedings of the National Academy of Sciences of the United States of America,    (31):   [PMID:17652170]

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