Recombinant Human MDC Protein, >97%(SDS-PAGE, HPLC), high purity

Features and benefits
  • Expression System: E. coli
  • Accession #: O00626
  • Protein Tag: No tag
  • Bioactivity: Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-100ng/ml.
  • Endotoxin Concentration: <1.0 EU/μg
Item Number
rp148631
Grouped product items
SKUSizeAvailabilityPrice Qty
rp148631-10μg
10μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$99.90
rp148631-50μg
50μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$389.90
rp148631-100μg
100μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$629.90
rp148631-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,999.90

Animal Free, >97%(SDS-PAGE, HPLC), Active, E.coli, No tag, 25-93aa

Basic Description

Product NameRecombinant Human MDC Protein, >97%(SDS-PAGE, HPLC), high purity
SynonymsA-152E5.1 | ABCD-1 | CC chemokine STCP-1 | C-C motif chemokine 22 | CCL22 | chemokine (C-C motif) ligand 22 | DC/B-CK | Macrophage-derived chemokine | MDC | MDCStimulated T-cell chemotactic protein 1 | MGC34554 | SCYA22MDC(1-69) | small inducible cytokine
GradeActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance
Product Description

Purity
>97% by SDS-PAGE and HPLC analyses.

Function
May play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. Chemotactic for monocytes, dendritic cells and natural killer cells. Mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes but has no chemoattractant activity for neutrophils, eosinophils, and resting T-lymphocytes. Binds to CCR4. Processed forms MDC(3-69), MDC(5-69) and MDC(7-69) seem not be active.

Specifications & PurityActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥97%(SDS-PAGE&HPLC)
Purity>97%(SDS-PAGE, HPLC)
BioactivityFully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-100ng/ml.
Endotoxin Concentration<1.0 EU/μg
Expression SystemE. coli
SpeciesHuman
Amino Acids25-93aa
SequenceGPYGANMEDSVCCRDYVRYRLPLRVVKHFYWTSDSCPRPGVVLLTFRDKEICADPRVPWVKMILNKLSQ
Protein TagNo tag
Accession #O00626
Predicted molecular weight8.1 kDa
SDS-PAGE8.1 kDa

Images

Recombinant Human MDC Protein (rp148631)-Protein Bioactivity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 10-100 ng/mL.

Recombinant Human MDC Protein (rp148631)-SDS-PAGE
Recombinant Human MDC Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining, showing a single band at 8.1 kDa.

Product Specifications

FormLyophilized
ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
Storage TempStore at -20°C
Shipped InIce chest + Ice pads
Stability And StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconst

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ23F0600419Certificate of AnalysisMar 26, 2024 rp148631
ZJ23F0600420Certificate of AnalysisMar 26, 2024 rp148631
ZJ23F0600421Certificate of AnalysisMar 26, 2024 rp148631

Related Documents

References

1. Imai T, Chantry D, Raport CJ, Wood CL, Nishimura M, Godiska R, Yoshie O, Gray PW.  (1998)  Macrophage-derived chemokine is a functional ligand for the CC chemokine receptor 4..  J Biol Chem,  273  (3): (1764-8).  [PMID:9430724] [10.1021/op500134e]
2. Vulcano M, Albanesi C, Stoppacciaro A, Bagnati R, D'Amico G, Struyf S, Transidico P, Bonecchi R, Del Prete A, Allavena P et al..  (2001)  Dendritic cells as a major source of macrophage-derived chemokine/CCL22 in vitro and in vivo..  Eur J Immunol,  31  (3): (812-22).  [PMID:11241286] [10.1021/op500134e]
3. Godiska R, Chantry D, Raport CJ, Sozzani S, Allavena P, Leviten D, Mantovani A, Gray PW.  (1997)  Human macrophage-derived chemokine (MDC), a novel chemoattractant for monocytes, monocyte-derived dendritic cells, and natural killer cells..  J Exp Med,  185  (9): (1595-604).  [PMID:9151897] [10.1021/op500134e]
4. Regenass P, Abboud D, Daubeuf F, Lehalle C, Gizzi P, Riché S, Hachet-Haas M, Rohmer F, Gasparik V, Boeglin D et al..  (2018)  Discovery of a Locally and Orally Active CXCL12 Neutraligand (LIT-927) with Anti-inflammatory Effect in a Murine Model of Allergic Airway Hypereosinophilia..  J Med Chem,  61  (17): (7671-7686).  [PMID:30106292] [10.1021/op500134e]
5. Chang, M s Ms and 7 more authors..  (1997)  Molecular cloning and functional characterization of a novel CC chemokine, stimulated T cell chemotactic protein (STCP-1) that specifically acts on activated T lymphocytes..  The Journal of biological chemistry,    (3):   [PMID:9312138]
6. Loftus, B J BJ and 18 more authors..  (1999)  Genome duplications and other features in 12 Mb of DNA sequence from human chromosome 16p and 16q..  Genomics,    (15):   [PMID:10493829]
7. Martin, Joel J and 121 more authors..  (2004)  The sequence and analysis of duplication-rich human chromosome 16..  Nature,    (23):   [PMID:15616553]

Solution Calculators