Click Here for 5% Off Your First Aladdin Purchase!
Call +1 (833) 552-7181 or Contact us Earn $25 in coupons on your first order with Aladdin!
Toggle Nav
My Cart
Search
Advanced Search
Menu
Sign In
྾
main product photo

Recombinant Human MIF Protein, ≥95%(SDS-PAGE), high purity

  • ActiBioPure™
  • Animal Free
  • Azide Free
  • Bioactive
  • Carrier Free
  • ≥95%(SDS-PAGE)
Features and benefits
  • Expression System: E. coli
  • Accession #: P14174
  • Protein Tag: No tag
  • Bioactivity: Fully biologically active when compared to standard. The specific activity is determined by binding rhCD74 in a functional ELISA.
  • Endotoxin Concentration: <1 EU/µg
Item Number
rp148808
Grouped product items
SKUSizeAvailabilityPrice Qty
rp148808-10μg
10μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$129.90
rp148808-50μg
50μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$399.90
rp148808-100μg
100μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$559.90
rp148808-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,999.90
Add to Wish List Compare
Bulk Order  DATASHEET

Animal Free, ≥95%(SDS-PAGE), Active, E.coli, No tag, 1-115 aa

View related series
Accession#:P14174 Gene ID:4282 MIF

Basic Description

Product NameRecombinant Human MIF Protein, ≥95%(SDS-PAGE), high purity
SynonymsEC 5.3.2.1; EC 5.3.3.12; GIFmacrophage migration inhibitory factor; GLIF; Glycosylation-inhibiting factor; L-dopachrome isomerase; L-dopachrome tautomerase; macrophage migration inhibitory factor (glycosylation-inhibiting factor); MIF; MMIF; Phenylpyruvat
GradeActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free
Product Description

Purity
≥95% SDS-PAGE. Recombinant human MIF, fused to His-tag at N-terminus, was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques.

Function
Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.

Specifications & PurityActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥95%(SDS-PAGE)
Purity≥95%(SDS-PAGE)
BioactivityFully biologically active when compared to standard. The specific activity is determined by binding rhCD74 in a functional ELISA.
Endotoxin Concentration<1 EU/µg
Expression SystemE. coli
SpeciesHuman
Amino Acids1-115 aa
SequenceMPMFIVNTNVPRASVPDGFLSELTQQLAQATGKPPQYIAVHVVPDQLMAFGGSSEPCALCSLHSIGKIGGAQNRSYSKLLCGLLAERLRISPDRVYINYYDMNAANVGWNNSTFA
Protein TagNo tag
Accession #P14174
Predicted molecular weight12.5 kDa
SDS-PAGE12.5 kDa

Images

Recombinant Human MIF protein (rp148808)-Protein Bioactivity
Fully biologically active when compared to standard. The specific activity is determined by binding rhCD74 in a functional ELISA.

Recombinant Human MIF protein (rp148808)-SDS-PAGE
Recombinant Human MIF protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 12.5 kDa.

Product Specifications

FormLyophilized
ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportio
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconst

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

3 results found

Lot NumberCertificate TypeDateItem
ZJ23F0600322Certificate of AnalysisMar 26, 2024 rp148808
ZJ23F0600323Certificate of AnalysisMar 26, 2024 rp148808
ZJ23F0600324Certificate of AnalysisMar 26, 2024 rp148808

Related Documents

 SDS

 Specification Sheet

FAQs and Articles

FAQ
Antibody structure and isotypes
Categories: Technical articles
Tags:
Immunoglobulin Antibody isotype Heavy chain and light chain

References

1. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K et al..  (2004)  Complete sequencing and characterization of 21,243 full-length human cDNAs..  Nat Genet,  36  (1): (40-5).  [PMID:14702039]
2. Leng L, Metz CN, Fang Y, Xu J, Donnelly S, Baugh J, Delohery T, Chen Y, Mitchell RA, Bucala R.  (2003)  MIF signal transduction initiated by binding to CD74..  J Exp Med,  197  (11): (1467-76).  [PMID:12782713]
3. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.  (2009)  Lysine acetylation targets protein complexes and co-regulates major cellular functions..  Science,  325  (5942): (834-40).  [PMID:19608861]
4. Trivedi-Parmar V, Jorgensen WL.  (2018)  Advances and Insights for Small Molecule Inhibition of Macrophage Migration Inhibitory Factor..  J Med Chem,  61  (18): (8104-8119).  [PMID:29812929]
5. Lubetsky JB, Swope M, Dealwis C, Blake P, Lolis E.  (1999)  Pro-1 of macrophage migration inhibitory factor functions as a catalytic base in the phenylpyruvate tautomerase activity..  Biochemistry,  38  (22): (7346-54).  [PMID:10353846]
6. Orita M, Yamamoto S, Katayama N, Aoki M, Takayama K, Yamagiwa Y, Seki N, Suzuki H, Kurihara H, Sakashita H et al..  (2001)  Coumarin and chromen-4-one analogues as tautomerase inhibitors of macrophage migration inhibitory factor: discovery and X-ray crystallography..  J Med Chem,  44  (4): (540-7).  [PMID:11170644]
7. Ouertatani-Sakouhi H, El-Turk F, Fauvet B, Cho MK, Pinar Karpinar D, Le Roy D, Dewor M, Roger T, Bernhagen J, Calandra T, Zweckstetter M, Lashuel HA.  ()  Identification and characterization of novel classes of macrophage migration inhibitory factor (MIF) inhibitors with distinct mechanisms of action..  J Biol Chem,      [PMID:20516071]
8. Gregg V Crichlow,Jodi B Lubetsky,Lin Leng,Richard Bucala,Elias J Lolis.  (2008-12-19)  Structural and kinetic analyses of macrophage migration inhibitory factor active site interactions..  Biochemistry,  48  ((1)): (132-139).  [PMID:19090677]
9. Alissa A Hare,Lin Leng,Sunilkumar Gandavadi,Xin Du,Zoe Cournia,Richard Bucala,William L Jorgensen.  (2010-08-24)  Optimization of N-benzyl-benzoxazol-2-ones as receptor antagonists of macrophage migration inhibitory factor (MIF)..  Bioorganic & medicinal chemistry letters,  20  ((19)): (5811-5814).  [PMID:20728358]
10. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge.  (2011-01-29)  Initial characterization of the human central proteome..  BMC systems biology,  5  (17-17).  [PMID:21269460]
11. William L Jorgensen,Alexander Trofimov,Xin Du,Alissa A Hare,Lin Leng,Richard Bucala.  (2011-07-02)  Benzisothiazolones as modulators of macrophage migration inhibitory factor..  Bioorganic & medicinal chemistry letters,  21  ((15)): (4545-4549).  [PMID:21719283]
12. Crichlow GV, Cheng KF, Dabideen D, Ochani M, Aljabari B, Pavlov VA, Miller EJ, Lolis E, Al-Abed Y..  (2007)  Alternative chemical modifications reverse the binding orientation of a pharmacophore scaffold in the active site of macrophage migration inhibitory factor..  J Biol Chem,  282  (32): (23089-23095).  [PMID:17526494]
13. Cournia Z, Leng L, Gandavadi S, Du X, Bucala R, Jorgensen WL..  (2009)  Discovery of human macrophage migration inhibitory factor (MIF)-CD74 antagonists via virtual screening..  J Med Chem,  52  (2): (416-424).  [PMID:19090668]
14. El Turk F, Fauvet B, Ouertatani-Sakouhi H, Lugari A, Betzi S, Roche P, Morelli X, Lashuel HA..  (2010)  An integrative in silico methodology for the identification of modulators of macrophage migration inhibitory factor (MIF) tautomerase activity..  Bioorg Med Chem,  18  (14): (5425-5440).  [PMID:20639113]
15. Jorgensen WL, Gandavadi S, Du X, Hare AA, Trofimov A, Leng L, Bucala R..  (2010)  Receptor agonists of macrophage migration inhibitory factor..  Bioorg Med Chem Lett,  20  (23): (7033-7036).  [PMID:20971005]
16. Alam A, Pal C, Goyal M, Kundu MK, Kumar R, Iqbal MS, Dey S, Bindu S, Sarkar S, Pal U, Maiti NC, Adhikari S, Bandyopadhyay U..  (2011)  Synthesis and bio-evaluation of human macrophage migration inhibitory factor inhibitor to develop anti-inflammatory agent..  Bioorg Med Chem,  19  (24): (7365-7373).  [PMID:22088307]
17. Dahlgren MK, Garcia AB, Hare AA, Tirado-Rives J, Leng L, Bucala R, Jorgensen WL..  (2012)  Virtual screening and optimization yield low-nanomolar inhibitors of the tautomerase activity of Plasmodium falciparum macrophage migration inhibitory factor..  J Med Chem,  55  (22): (10148-10159).  [PMID:23067344]
18. Yu WL, Jones BD, Kang M, Hammons JC, La Clair JJ, Burkart MD..  (2013)  Spirohexenolide A targets human macrophage migration inhibitory factor (hMIF)..  J Nat Prod,  76  (5): (817-823).  [PMID:23659282]
19. Pantouris G, Rajasekaran D, Garcia AB, Ruiz VG, Leng L, Jorgensen WL, Bucala R, Lolis EJ..  (2014)  Crystallographic and receptor binding characterization of Plasmodium falciparum macrophage migration inhibitory factor complexed to two potent inhibitors..  J Med Chem,  57  (20): (8652-8656).  [PMID:25268646]
20. Spencer ES, Dale EJ, Gommans AL, Rutledge MT, Vo CT, Nakatani Y, Gamble AB, Smith RA, Wilbanks SM, Hampton MB, Tyndall JD..  (2015)  Multiple binding modes of isothiocyanates that inhibit macrophage migration inhibitory factor..  Eur J Med Chem,  93  (501-510).  [PMID:25743213]
21. Le Hiress M, Akagah B, Bernadat G, Tu L, Thuillet R, Huertas A, Phan C, Fadel E, Simonneau G, Humbert M, Jalce G, Guignabert C..  (2018)  Design, Synthesis, and Biological Activity of New N-(Phenylmethyl)-benzoxazol-2-thiones as Macrophage Migration Inhibitory Factor (MIF) Antagonists: Efficacies in Experimental Pulmonary Hypertension..  J Med Chem,  61  (7): (2725-2736).  [PMID:29526099]
22. Kok T, Wapenaar H, Wang K, Neochoritis CG, Zarganes-Tzitzikas T, Proietti G, Eleftheriadis N, Kurpiewska K, Kalinowska-Tłuścik J, Cool RH, Poelarends GJ, Dömling A, Dekker FJ..  (2018)  Discovery of chromenes as inhibitors of macrophage migration inhibitory factor..  Bioorg Med Chem,  26  (5): (999-1005).  [PMID:29428527]
23. Cirillo PF,Asojo OA,Khire U,Lee Y,Mootien S,Hegan P,Sutherland AG,Peterson-Roth E,Ledizet M,Koski RA,Anthony KG.  (2020)  Inhibition of Macrophage Migration Inhibitory Factor by a Chimera of Two Allosteric Binders..  ACS Med Chem Lett,  11  (10.0): (1843-1847).  [PMID:33062162]
24. Xiao Z,Fokkens M,Chen D,Kok T,Proietti G,van Merkerk R,Poelarends GJ,Dekker FJ.  (2020)  Structure-activity relationships for binding of 4-substituted triazole-phenols to macrophage migration inhibitory factor (MIF)..  Eur J Med Chem,  186  (111849-111849).  [PMID:31767137]
25. Hochstrasser, D F DF and 9 more authors..  (1992)  Human liver protein map: a reference database established by microsequencing and gel comparison..  Electrophoresis,      [PMID:1286669]
26. Weiser, W Y WY and 5 more authors..  (1989)  Molecular cloning of a cDNA encoding a human macrophage migration inhibitory factor..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:2552447]
27. Wistow, G J GJ, Shaughnessy, M P MP, Lee, D C DC, Hodin, J J and Zelenka, P S PS..  (1993)  A macrophage migration inhibitory factor is expressed in the differentiating cells of the eye lens..  Proceedings of the National Academy of Sciences of the United States of America,    (15):   [PMID:7679497]
28. Zeng, F Y FY and 5 more authors..  (1993)  The major binding protein of the interferon antagonist sarcolectin in human placenta is a macrophage migration inhibitory factor..  Archives of biochemistry and biophysics,    (15):   [PMID:7683862]
29. Bernhagen, J J and 5 more authors..  (1994)  Purification, bioactivity, and secondary structure analysis of mouse and human macrophage migration inhibitory factor (MIF)..  Biochemistry,    (29):   [PMID:7947826]
30. Paralkar, V V and Wistow, G G..  (1994)  Cloning the human gene for macrophage migration inhibitory factor (MIF)..  Genomics,    (1):   [PMID:8188240]
31. Mikayama, T T and 9 more authors..  (1993)  Molecular cloning and functional expression of a cDNA encoding glycosylation-inhibiting factor..  Proceedings of the National Academy of Sciences of the United States of America,    (1):   [PMID:8234256]
32. Kato, Y Y and 7 more authors..  (1996)  The crystal structure of human glycosylation-inhibiting factor is a trimeric barrel with three 6-stranded beta-sheets..  Proceedings of the National Academy of Sciences of the United States of America,    (2):   [PMID:8610159]
33. Sun, H W HW, Bernhagen, J J, Bucala, R R and Lolis, E E..  (1996)  Crystal structure at 2.6-A resolution of human macrophage migration inhibitory factor..  Proceedings of the National Academy of Sciences of the United States of America,    (28):   [PMID:8643551]
34. Sugimoto, H H, Suzuki, M M, Nakagawa, A A, Tanaka, I I and Nishihira, J J..  (1996)  Crystal structure of macrophage migration inhibitory factor from human lymphocyte at 2.1 A resolution..  FEBS letters,    (1):   [PMID:8766818]
35. Kleemann, R R and 13 more authors..  (2000)  Intracellular action of the cytokine MIF to modulate AP-1 activity and the cell cycle through Jab1..  Nature,    (9):   [PMID:11089976]
36. .  (2001)  A novel 5'-flanking region polymorphism of macrophage migration inhibitory factor is associated with systemic-onset juvenile idiopathic arthritis..  Arthritis and rheumatism,      [PMID:11508429]
37. Gevaert, Kris K and 6 more authors..  (2003)  Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides..  Nature biotechnology,      [PMID:12665801]
38. Shen, Li L and 7 more authors..  (2003)  The apoptosis-associated protein BNIPL interacts with two cell proliferation-related proteins, MIF and GFER..  FEBS letters,    (10):   [PMID:12681488]
39. Collins, John E JE and 12 more authors..  (2004)  A genome annotation-driven approach to cloning the human ORFeome..  Genome biology,      [PMID:15461802]
40. Emonts, Marieke M and 10 more authors..  (2007)  Association between high levels of blood macrophage migration inhibitory factor, inappropriate adrenal response, and early death in patients with severe sepsis..  Clinical infectious diseases : an official publication of the Infectious Diseases Society of America,    (15):   [PMID:17443469]
41. Merk, Melanie M and 13 more authors..  (2009)  The Golgi-associated protein p115 mediates the secretion of macrophage migration inhibitory factor..  Journal of immunology (Baltimore, Md. : 1950),    (1):   [PMID:19454686]
42. Bienvenut, Willy V WV and 6 more authors..  (2012)  Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features..  Molecular & cellular proteomics : MCP,      [PMID:22223895]
43. Alam, Athar A and 12 more authors..  (2012)  Novel anti-inflammatory activity of epoxyazadiradione against macrophage migration inhibitory factor: inhibition of tautomerase and proinflammatory activities of macrophage migration inhibitory factor..  The Journal of biological chemistry,    (13):   [PMID:22645149]
44. Van Damme, Petra P and 15 more authors..  (2012)  N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB..  Proceedings of the National Academy of Sciences of the United States of America,    (31):   [PMID:22814378]
45. Fan, Chengpeng C and 7 more authors..  (2013)  MIF intersubunit disulfide mutant antagonist supports activation of CD74 by endogenous MIF trimer at physiologic concentrations..  Proceedings of the National Academy of Sciences of the United States of America,    (2):   [PMID:23776208]
46. Bian, Yangyang Y and 9 more authors..  (2014)  An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome..  Journal of proteomics,    (16):   [PMID:24275569]

Solution Calculators

Privacy Policy Website Terms of Use Return policy Terms and Conditions of Sale Cookie Policy Sales Policy
Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.
Copyright © 2023-present Aladdin Scientific Corp . All rights reserved.