Recombinant Human NRG1/HRG1 Protein, ＞92%(SDS-PAGE), high purity

Item Number

rp149423

• Product Name: Recombinant Human NRG1/HRG1 Protein, ＞92%(SDS-PAGE), high purity
• Synonyms: Acetylcholine receptor-inducing activity; Acetylcholine receptor-inducing activity, CHICK, homolog of; ARIA; Breast cancer cell differentiation factor p45; GGF; GGF2; Glial growth factor; Heregulin; heregulin, alpha (45kD, ERBB2 p185-activator); HGL; HRG
• Grade: ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance
• Specifications & Purity: ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE)
• Purity: ＞92%(SDS-PAGE)
• Bioactivity: Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. The ED₅₀ for this effect is 20‑100 ng/mL.
• Endotoxin Concentration: ＜0.1 EU/μg
• Expression System: HEK293
• Species: Human
• Amino Acids: 20-241 aa
• Sequence: SGKKPESAAGSQSPALPPRLKEMKSQESAAGSKLVLRCETSSEYSSLRFKWFKNGNELNRKNKPQNIKIQKKPGKSELRINKASLADSGEYMCKVISKLGNDSASANITIVESNEIITGMPASTEGAYVSSESPIRISVSTEGANTSSSTSTSTTGTSHLVKCAEKEKTFCVNGGECFMVKDLSNPSRYLCKCQPGFTGARCTENVPMKVQNQEKAEELYQKHHHHHH
• Protein Tag: C-His
• Accession #: Q02297.1
• Predicted molecular weight: 24.8 kDa
• SDS-PAGE: 40-43 kDa, under reducing conditions

Product Description

Purity
≥95% (SDS-PAGE)
Endotoxin level
<0.1 EU/ug
Function
Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development.