| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| rp149785-10μg | 10μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $79.90 | |
| rp149785-50μg | 50μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $209.90 | |
| rp149785-100μg | 100μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $319.90 | |
| rp149785-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,999.90 |
Animal Free, >97% (SDS-PAGE&HPLC), Active, E. coli, No tag, 32-115 aa
| Product Name | Recombinant Human Parathyroid Hormone Protein, >97% (SDS-PAGE&HPLC), high purity |
|---|---|
| Synonyms | hPTH; Parathormone; Parathyrin; Parathyroid hormone; Parathyroid hormone 1; Prepro PTH; Preproparathyroid hormone; PTH; PTH1; PTH1 receptor; PTH1R; PTHR; PTHR1; PTHY_HUMAN |
| Grade | ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance |
| Specifications & Purity | ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥97%(SDS-PAGE&HPLC) |
| Biochemical and Physiological Mechanisms | PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells. |
| Purity | >97% (SDS-PAGE&HPLC) |
| Bioactivity | Fully biologically active when compared to standard. The ED50 as determined by its ability to induce cAMP accumulation in murine MC3T3E1 cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 × 104 IU/mg. |
| Endotoxin Concentration | ＜1.0 EU/μg |
| Expression System | E. coli |
| Species | Human |
| Amino Acids | 32-115 aa |
| Sequence | SVSEIQLMHNLGKHLNSMERVEWLRKKLQDVHNFVALGAPLAPRDAGSQRPRKKEDNVLVESHEKSLGEADKADVNVLTKAKSQ |
| Protein Tag | No tag |
| Accession # | P01270 |
| Predicted molecular weight | 9.4 kDa |
| SDS-PAGE | 9.4 kDa |
Recombinant Human Parathyroid Hormone Protein (rp149785) - Protein Bioactivity
Fully biologically active when compared to standard. The ED₅₀ as determined by its ability to induce cAMP accumulation in murine MC3T3E1 cells is less than 50 ng/mL, corresponding to a specific activity of > 2.0 × 10⁴ IU/mg.
Recombinant Human Parathyroid Hormone Protein (rp149785) - SDS-PAGE
Recombinant Human Parathyroid Hormone Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 9.4 kDa.
| Form | Lyophilized |
|---|---|
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportio |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution. Upon delivery aliquot. Avoid freeze/thaw cycle. |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1001337 | Certificate of Analysis | Oct 19, 2023 | rp149785 |
| ZJ23F1001338 | Certificate of Analysis | Oct 19, 2023 | rp149785 |
| ZJ23F1001339 | Certificate of Analysis | Oct 19, 2023 | rp149785 |
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| 2. Keutmann, H T HT, Sauer, M M MM, Hendy, G N GN, O'Riordan, L H LH and Potts, J T JT.. (1978) Complete amino acid sequence of human parathyroid hormone.. Biochemistry, (26): [PMID:728431] |
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| 8. Andreatta, R H RH and 7 more authors.. (1973) [Synthesis of sequence 1-34 of human parathyroid hormone].. Helvetica chimica acta, [PMID:4721748] |
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| 16. Marx, U C UC, Adermann, K K, Bayer, P P, Forssmann, W G WG and Rösch, P P.. (2000) Solution structures of human parathyroid hormone fragments hPTH(1-34) and hPTH(1-39) and bovine parathyroid hormone fragment bPTH(1-37).. Biochemical and biophysical research communications, (7): [PMID:10623601] |
| 17. Jin, L L and 8 more authors.. (2000) Crystal structure of human parathyroid hormone 1-34 at 0.9-A resolution.. The Journal of biological chemistry, (1): [PMID:10837469] |
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