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Recombinant Human PDGF-BB GMP Protein

  • ActiBioPure™
  • Animal Free
  • Bioactive
  • Carrier Free
  • GMP
  • 高性能
  • ≥75%(SDS-PAGE&SEC-HPLC)
Features and benefits
  • Expression System: E. coli
  • Protein Tag: No tag
  • Bioactivity: Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. The ED50 for this effect is 1.5-6 ng/mL. The specific activity of recombinant human PDGF-BB is >7.5 x 10^5 IU/mg, which is calibrated against the human PDGF-BB WHO International
  • Endotoxin Concentration: <0.01 EU/μg
Item Number
rp168345-GMP
Grouped product items
SKUSizeAvailabilityPrice Qty
rp168345-GMP-50μg
50μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,199.00
rp168345-GMP-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$9,599.00

GMP, ≥97% (SDS-PAGE&SEC-HPLC), Active, E. coli, No tag, 82-190 aa

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Basic Description

Product NameRecombinant Human PDGF-BB GMP Protein
GradeActiBioPure™, Animal Free, Bioactive, Carrier Free, GMP, 高性能
Specifications & PurityAnimal Free, Carrier Free, GMP, Bioactive, ActiBioPure™, High performance, ≥75%(SDS-PAGE&SEC-HPLC)
Biochemical and Physiological MechanismsGrowth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin (PubMed:26599395). Required for normal proliferation and rec
BioactivityMeasured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. The ED50 for this effect is 1.5-6 ng/mL. The specific activity of recombinant human PDGF-BB is >7.5 x 10^5 IU/mg, which is calibrated against the human PDGF-BB WHO International
Endotoxin Concentration<0.01 EU/μg
Expression SystemE. coli
Amino Acids82-190 aa
SequenceSLGSLTIAEPAMIAECKTRTEVFEISRRLIDRTNANFLVWPPCVEVQRCSGCCNNRNVQCRPTQVQLRPVQVRKIEIVRKKPIFKKATVTLEDHLACKCETVAAARPVT
Protein TagNo tag
N-terminal SequenceSer-Leu-Gly-Ser-Leu-Thr-Ile-Ala-Glu-Pro
Predicted molecular weight12.3 kDa (monomer)
SDS-PAGE13 kDa, under reducing conditions

Product Specifications

ReconstitutionReconstitute at 100 μg/mL in 4 mM HCl.
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageA minimum of 12 months when stored at ≤ -20 ℃ as supplied. 1 month, 2 to 8 ℃ under sterile conditions after reconstitution. 3 months, ≤ -28 ℃ under sterile conditions after reconstitution. Upon delivery aliquot. Avoid freeze/thaw cycle.

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