| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| rp150291-10μg | 10μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $69.90 | |
| rp150291-50μg | 50μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $279.90 | |
| rp150291-100μg | 100μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $449.90 | |
| rp150291-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,569.90 |
Animal Free, >90% (SDS-PAGE), Active, HEK293, C-His tag, 22-441 aa
| Product Name | Recombinant Human PLA2G7 Protein, ＞90% (SDS-PAGE), high purityused for Active,SDS-PAGE various applications |
|---|---|
| Synonyms | 2-acetyl-1-alkylglycerophosphocholine esterase; EC 3.1.1; EC 3.1.1.47,1-alkyl-2-acetylglycerophosphocholine esterase; Group-VIIA phospholipase A2; gVIIA-PLA2; LDL-associated phospholipase A2; LDL-PLA(2); LDL-PLA2; lipoprotein-associated phospholipase A2; |
| Grade | ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free |
| Product Description | Purity >90% (SDS-PAGE) Function Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids. |
| Specifications & Purity | ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥90%(SDS-PAGE) |
| Purity | ＞90% (SDS-PAGE) |
| Bioactivity | Measured by its ability to cleave a colorimetric peptide substrate, 1O-hexadecyl-2-deoxy-2-thio Sacetylsnglyceryl-3-phosphoryl choline (2- Thio-PAF), in the presence of 5, 5’Dithiobis(2-nitrobenzoic acid) (DTNB). The specific activity is >5000 pmoles/min/ |
| Endotoxin Concentration | <1.0 EU/μg |
| Expression System | HEK293 |
| Species | Human |
| Amino Acids | 22-441 aa |
| Sequence | FDWQYINPVAHMKSSAWVNKIQVLMAAASFGQTKIPRGNGPYSVGCTDLMFDHTNKGTFLRLYYPSQDNDRLDTLWIPNKEYFWGLSKFLGTHWLMGNILRLLFGSMTTPANWNSPLRPGEKYPLVVFSHGLGAFRTLYSAIGIDLASHGFIVAAVEHRDRSASATYYFKDQSAAEIGDKSWLYLRTLKQEEETHIRNEQVRQRAKECSQALSLILDIDHGKPVKNALDLKFDMEQLKDSIDREKIAVIGHSFGG |
| Protein Tag | C-His |
| Protein Length | Full length protein |
| Source | Recombinant |
| Predicted molecular weight | 49.2 kDa |
| SDS-PAGE | 50-55 kDa, under reducing conditions |
Recombinant Human PLA2G7 Protein (rp150291) - Enzyme Activity
Measured by its ability to cleave a colorimetric peptide substrate, 1-O-hexadecyl-2-deoxy-2-thio-S-acetyl-sn-glyceryl-3-phosphorylcholine (2-Thio-PAF) (T334670), in the presence of 5,5’Dithio-bis(2-nitrobenzoic acid) (DTNB) (D425581). The specific activity is >5,000 pmol/min/ug, as measured under the described condition.
Recombinant Human PLA2G7 Protein (rp150291) - SDS-PAGE
3 μg/lane of Recombinant Human PLA2G7 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 54.2 kDa.
| Application | Active,SDS-PAGE |
|---|---|
| Form | Lyophilized |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.27mg/mL. |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store it under sterile conditions at -20℃ to -80℃ upon receiving. Upon delivery aliquot. Avoid freeze/thaw cycle. |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0900996 | Certificate of Analysis | Sep 11, 2023 | rp150291 |
| ZJ23F0900997 | Certificate of Analysis | Sep 11, 2023 | rp150291 |
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