| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| rp150851-10μg | 10μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $59.90 | |
| rp150851-50μg | 50μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $139.90 | |
| rp150851-100μg | 100μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $209.90 | |
| rp150851-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $1,499.90 |
Animal Free, >98%(SDS-PAGE), Active, E. coli, No tag, 29-202 aa
| Product Name | Recombinant Human RANK Protein, ＞98%(SDS-PAGE), high purity |
|---|---|
| Synonyms | CD 265; CD265; FEO; LOH18CR1; Loss of heterozygosity 18 chromosomal region 1; mRANK; ODFR; OFE; OPTB7; Osteoclast differentiation factor receptor; OSTS; Paget disease of bone 2; PDB 2; PDB2; RANK; Receptor activator of NF KB; Receptor activator of NF-KB; |
| Grade | ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance |
| Product Description | Purity |
| Specifications & Purity | ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥98%(SDS-PAGE) |
| Purity | ＞98%(SDS-PAGE) |
| Bioactivity | Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit sRANK Ligand induced nuclear factor kappa B(NFkappaB) in RAW 264.7 cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 × 104 IU/mg |
| Endotoxin Concentration | ＜0.1 EU/μg |
| Expression System | E.coli |
| Species | Human |
| Amino Acids | 29-202 aa |
| Sequence | QIAPPCTSEK HYEHLGRCCN KCEPGKYMSS KCTTTSDSVC LPCGPDEYLD SWNEEDKCLL HKVCDTGKAL VAVVAGNSTT PRRCACTAGY HWSQDCECCR RNTECAPGLG AQHPLQLNKD TVCKPCLAGY FSDAFSSTDK CRPWTNCTFL GKRVEHHGTE KSDAVCSSSL PARK |
| Protein Tag | No tag |
| Accession # | Q9Y6Q6 |
| Predicted molecular weight | 19.1 kDa |
| SDS-PAGE | 20.5 kDa, under reducing conditions; 18.2 kDa, under non-reducing conditions. |
Recombinant Human RANK protein (rp150851) - SDS-PAGE
Recombinant Human RANK protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 20.5 kDa under reducing conditions and 18.2 kDa under non-reducing conditions.
Recombinant Human RANK protein (rp150851) - Protein Bioactivity
Fully biologically active when compared to standard. The ED₅₀ as determined by its ability to inhibit RANK Ligand induced nuclear factor kappa B(NFkappaB) in RAW 264.7 cells is less than 50 ng/mL, corresponding to a specific activity of > 2.0 × 10⁴ IU/mg in the presence of 15 ng/mL of recombinant RANK Ligand.
| Form | Lyophilized |
|---|---|
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportio |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 2-8 ℃ for one month. Aliquot and store at -80 ℃ for 12 months.Upon delivery aliquot. Avoid freeze/thaw cycle. |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0700627 | Certificate of Analysis | Jul 21, 2023 | rp150851 |
| ZJ23F0700625 | Certificate of Analysis | Jul 20, 2023 | rp150851 |
| ZJ23F0700626 | Certificate of Analysis | Jul 20, 2023 | rp150851 |
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| 2. Wong, B R BR and 5 more authors.. (1998) The TRAF family of signal transducers mediates NF-kappaB activation by the TRANCE receptor.. The Journal of biological chemistry, (23): [PMID:9774460] |
| 3. Nakagawa, N N and 7 more authors.. (1998) RANK is the essential signaling receptor for osteoclast differentiation factor in osteoclastogenesis.. Biochemical and biophysical research communications, (18): [PMID:9878548] |
| 4. Hughes, A E AE and 10 more authors.. (2000) Mutations in TNFRSF11A, affecting the signal peptide of RANK, cause familial expansile osteolysis.. Nature genetics, [PMID:10615125] |
| 5. Wada, Teiji T and 6 more authors.. (2005) The molecular scaffold Gab2 is a crucial component of RANK signaling and osteoclastogenesis.. Nature medicine, [PMID:15750601] |
| 6. Guerrini, Matteo M MM and 20 more authors.. (2008) Human osteoclast-poor osteopetrosis with hypogammaglobulinemia due to TNFRSF11A (RANK) mutations.. American journal of human genetics, [PMID:18606301] |
| 7. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648] |
| 8. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 9. Sirinian, Chaido C, Papanastasiou, Anastasios D AD, Zarkadis, Ioannis K IK and Kalofonos, Haralabos P HP.. (2013) Alternative splicing generates a truncated isoform of human TNFRSF11A (RANK) with an altered capacity to activate NF-κB.. Gene, (1): [PMID:23664977] |
