Recombinant Human S100B Protein, >95%(SDS-PAGE), high purity

Features and benefits
  • Expression System: E. coli
  • Protein Tag: N-His
  • Bioactivity: Testing in progress
Item Number
rp156671
Grouped product items
SKUSizeAvailabilityPrice Qty
rp156671-10μg (Trial Size)
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10μg
In stock
$59.90
rp156671-50μg
50μg
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$139.90
rp156671-100μg
100μg
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Production requires sourcing of materials. We appreciate your patience and understanding.
$219.90
rp156671-1mg
1mg
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$2,599.90

Carrier Free, >95%(SDS-PAGE), E.coli, N-His tag, 1-92 aa

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Accession#:P04271 Gene ID:6285 S100B

Basic Description

Product NameRecombinant Human S100B Protein, >95%(SDS-PAGE), high purity
Synonymsbeta (neural); NEF; S100 beta; S100 calcium binding protein B; S100 calcium-binding protein B; S100 calcium-binding protein, beta (neural); S-100 calcium-binding protein, beta chain, 10protein S100-B; S-100 protein beta chain; S-100 protein subunit beta;
GradeActiBioPure™, Azide Free, Bioactive, Carrier Free
Product Description

Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining. 

Description: 

100B, previously called S100 beta, belongs to the S100 family within the EF-hand superfamily of Ca2+ binding proteins. S100 proteins contain two EF-hand motifs that differ in affinity, separated by a hinge region with a hydrophobic cleft that is exposed upon Ca2+ binding. S100B is a 91 amino acid (aa) protein, after removal of the initial methionine, and is found as homodimers of 10.4 kDa monomers. Human S100B shares 99%, 98%, 100%, 99% and 97% aa sequence identity with mouse, rat, rabbit, equine and bovine S100B, respectively. Within the S100 family, human S100B shows the highest aa identity (59%) with S100A1. S100B is expressed primarily by astrocytes and oligodendrocytes in the central nervous system, and by Schwann cells in the peripheral nervous system. Ca2+-bound S100B interacts in vitro with at least 20 cytoplasmic proteins, including several structural molecules such as tubulin and GFAP. It can inhibit the phosphorylation of these kinase substrates and others such as tau and neuromodulin. Astrocytes can secrete S100B, which then acts in a cytokine-like manner. Nanomolar concentrations of S100B are secreted constitutively, promote proliferation, and are neurotrophic and anti-apoptotic. Blood levels of S100B reflect extracellular concentrations within the nervous system, and are elevated in Down’s syndrome, Alzheimer’s disease and Tourette’s syndrome, metabolic stress, acute brain injury and brain tumors. Micromolar concentrations of S100B can be destructive and pro-apoptotic; they induce the expression of iNOS, COX-2, IL-1, IL‑6 and TNF-alpha by microglia, astrocytes or neurons. Most extracellular actions of S100B can be mediated by RAGE (receptor for advanced glycation end products), which is also a receptor for other S100 proteins.

Specifications & PurityActiBioPure™, Bioactive, Carrier Free, Azide Free, ≥95%(SDS-PAGE)
Purity>95%(SDS-PAGE)
BioactivityTesting in progress
Expression SystemE. coli
Amino Acids1-92 aa
SequenceMGHHHHHHMSELEKAMVALIDVFHQYSGREGDKHKLKKSELKELINNELSHFLEEIKEQEVVDKVMETLDNDGDGECDFQEFMAFVAMVTTACHEFFEHE
Protein TagN-His
Predicted molecular weight11.7 kDa
SDS-PAGE10.6 kDa, reducing conditions; 10.6 kDa, non-reducing conditions

Images

Recombinant Human S100B protein (rp156671) - SDS-PAGE
Recombinant Human S100B protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 10.6 kDa.

Product Specifications

FormLyophilized
ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilu
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -20°C for 1 year. Avoid freeze/thaw cycle.

Certificates

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2 results found

Lot NumberCertificate TypeDateItem
ZJ23F1101765Certificate of AnalysisDec 01, 2023 rp156671
ZJ23R1100150Certificate of AnalysisNov 15, 2023 rp156671

Related Documents

References

1. Markowitz J, Chen I, Gitti R, Baldisseri DM, Pan Y, Udan R, Carrier F, MacKerell AD, Weber DJ..  (2004)  Identification and characterization of small molecule inhibitors of the calcium-dependent S100B-p53 tumor suppressor interaction..  J Med Chem,  47  (21): (5085-5093).  [PMID:15456252]
2. Yoshimura C, Miyafusa T, Tsumoto K..  (2013)  Identification of small-molecule inhibitors of the human S100B-p53 interaction and evaluation of their activity in human melanoma cells..  Bioorg Med Chem,  21  (5): (1109-1115).  [PMID:23375094]
3. Allore, R J RJ and 6 more authors..  (1990)  Cloning and expression of the human S100 beta gene..  The Journal of biological chemistry,    (15):   [PMID:2394738]
4. Jensen, R R, Marshak, D R DR, Anderson, C C, Lukas, T J TJ and Watterson, D M DM..  (1985)  Characterization of human brain S100 protein fraction: amino acid sequence of S100 beta..  Journal of neurochemistry,      [PMID:4031854]
5. Baudier, J J, Glasser, N N, Haglid, K K and Gerard, D D..  (1984)  Purification, characterization and ion binding properties of human brain S100b protein..  Biochimica et biophysica acta,    (23):   [PMID:6487634]
6. Smith, S P SP and Shaw, G S GS..  (1998)  A novel calcium-sensitive switch revealed by the structure of human S100B in the calcium-bound form..  Structure (London, England : 1993),    (15):   [PMID:9519411]
7. Yang, Q Q, O'Hanlon, D D, Heizmann, C W CW and Marks, A A..  (1999)  Demonstration of heterodimer formation between S100B and S100A6 in the yeast two-hybrid system and human melanoma..  Experimental cell research,    (1):   [PMID:9925766]
8. Hattori, M M and 64 more authors..  (2000)  The DNA sequence of human chromosome 21..  Nature,    (18):   [PMID:10830953]
9. McClintock, Kimberly A KA and Shaw, Gary S GS..  (2003)  A novel S100 target conformation is revealed by the solution structure of the Ca2+-S100B-TRTK-12 complex..  The Journal of biological chemistry,    (21):   [PMID:12480931]
10. Lee, Tih-Shih TS and 12 more authors..  ()  Gene expression in temporal lobe epilepsy is consistent with increased release of glutamate by astrocytes..  Molecular medicine (Cambridge, Mass.),      [PMID:17515952]
11. Marlatt, Nicole M NM and Shaw, Gary S GS..  (2007)  Amide exchange shows calcium-induced conformational changes are transmitted to the dimer interface of S100B..  Biochemistry,    (26):   [PMID:17536784]
12. Liang, Jie J and 15 more authors..  (2007)  Differential expression of calcium-related genes in gastric cancer cells transfected with cellular prion protein..  Biochemistry and cell biology = Biochimie et biologie cellulaire,      [PMID:17612632]
13. Friel, Lara A LA and 9 more authors..  (2007)  The calcium binding protein, S100B, is increased in the amniotic fluid of women with intra-amniotic infection/inflammation and preterm labor with intact or ruptured membranes..  Journal of perinatal medicine,      [PMID:17624933]
14. Gilquin, Benoît B and 11 more authors..  (2010)  The calcium-dependent interaction between S100B and the mitochondrial AAA ATPase ATAD3A and the role of this complex in the cytoplasmic processing of ATAD3A..  Molecular and cellular biology,      [PMID:20351179]
15. Park, Hajeung H, Adsit, Floyd G FG and Boyington, Jeffrey C JC..  (2010)  The 1.5 Å crystal structure of human receptor for advanced glycation endproducts (RAGE) ectodomains reveals unique features determining ligand binding..  The Journal of biological chemistry,    (24):   [PMID:20943659]
16. Ostendorp, Thorsten T, Diez, Joachim J, Heizmann, Claus W CW and Fritz, Günter G..  (2011)  The crystal structures of human S100B in the zinc- and calcium-loaded state at three pH values reveal zinc ligand swapping..  Biochimica et biophysica acta,      [PMID:20950652]
17. Yamaguchi, Fuminori F and 6 more authors..  (2012)  S100 proteins modulate protein phosphatase 5 function: a link between CA2+ signal transduction and protein dephosphorylation..  The Journal of biological chemistry,    (20):   [PMID:22399290]

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