| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| rp181294-10μg | 10μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $99.90 | |
| rp181294-50μg | 50μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| rp181294-100μg | 100μg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $459.90 | |
| rp181294-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,799.90 |
Animal Free, ≥95% (SDS-PAGE), Active, 293F cell, C-His tag, 1-402 aa
| Product Name | Recombinant Human Serpin E1/PAI-1 Protein |
|---|---|
| Synonyms | Endothelial plasminogen activator inhibitor | Nexin | PAI1 | PAI-1 | PAI1PAI-1 | PAISerpin E1 | PLANH1 | PLANH1plasminogen activator inhibitor 1 | serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 1 |
| Grade | ActiBioPure™, Animal Free, Bioactive, Carrier Free |
| Product Description |
As a member of the Serpin superfamily of serine protease inhibitors, Serpin E1/PAI 1 is the principal inhibitor of urokinase type plasminogen activator (uPA) and tissue type PA. As important regulators of extracellular matrix remodeling, uPA and PAI 1 play a major role in many processes such as angiogenesis, tumor invasion, and obesity. For example, uPA and PAI-1 are the only tumor prognostic factors validated at the highest level of evidence with regard to their clinical utility in breast cancer. The human PAI-1 is initially synthesized as 402 amino acid precursor with an N terminal signal peptide. PAI 1 may exist in one of two possible conformations, designated as active or latent. The purified rhPAI 1 is active against rhuPA. The heterogeneity at the N terminus of the purified rhPAI 1 has been observed before for both the recombinant and native proteins.
|
| Specifications & Purity | ActiBioPure™, Bioactive, Animal Free, Carrier Free, ≥95%(SDS-PAGE) |
| Bioactivity | Immobilized Recombinant Human Serpin E1/PAI-1 Protein (rp181294) at 2.0 μg/mL can bind PAI1 Mouse mAb (Ab120074) with the EC50 of 75 ng/mL. |
| Endotoxin Concentration | ＜1.0 EU/μg |
| Expression System | HEK293 |
| Amino Acids | 1-402 aa |
| Sequence | MQMSPALTCLVLGLALVFGEGSAVHHPPSYVAHLASDFGVRVFQQVAQASKDRNVVFSPYGVASVLAMLQLTTGGETQQQIQAAMGFKIDDKGMAPALRHLYKELMGPWNKDEISTTDAIFVQRDLKLVQGFMPHFFRLFRSTVKQVDFSEVERARFIINDWVKTHTKGMISNLLGKGAVDQLTRLVLVNALYFNGQWKTPFPDSSTHRRLFHKSDGSTVSVPMMAQTNKFNYTEFTTPDGHYYDILELPYHGDT |
| Protein Tag | C-His |
| Predicted molecular weight | 44.1 kDa |
| SDS-PAGE | 48.3 kDa, under reducing condition; 48.9 kDa, under non-reducing condition |
Recombinant Human Serpin E1/PAI-1 Protein (rp181294) - ELISA
Immobilized Recombinant Human Serpin E1/PAI-1 Protein (rp181294) at 2.0 μg/mL can bind PAI1 Mouse mAb (Ab120074) with the EC50 of 75 ng/mL.
Recombinant Human Serpin E1/PAI-1 Protein (rp181294) - SDS-PAGE
3 μg/lane of Recombinant Human Serpin E1/PAI-1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 48.3kDa.
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilu |
|---|---|
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at -20°C for 1 year. Avoid freeze / thaw cycle. |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0707699 | Certificate of Analysis | Jul 05, 2024 | rp181294 |
| ZJ24F0707700 | Certificate of Analysis | Jul 05, 2024 | rp181294 |
| ZJ24F0707705 | Certificate of Analysis | Jul 05, 2024 | rp181294 |
| ZJ24R0600645 | Certificate of Analysis | Jun 10, 2024 | rp181294 |
