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Recombinant Human Serpin G1 Protein, ≥95% (SDS-PAGE), high purity

Features and benefits
  • Expression System: CHO
  • Accession #: P05155
  • Protein Tag: C-His
  • Bioactivity: Measured by its ability to inhibit Recombinant Human Complement Component C1s cleavage of a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-K-SBzl). The IC50 is <5 nM, as measured under the described conditions.
  • Endotoxin Concentration: <0.1 EU/μg
Item Number
rp151443
Grouped product items
SKUSizeAvailabilityPrice Qty
rp151443-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$59.90
rp151443-50μg
50μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$89.90
rp151443-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$109.90
rp151443-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$879.90
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Animal Free, ≥95% (SDS-PAGE), Active, CHO, C-His tag, 23-500 aa

View related series
Click to view protein information: SERPING1
Accession#: P05155 Gene ID: 710 Serpin G1 SERPING1

Basic Description

Product NameRecombinant Human Serpin G1 Protein, ≥95% (SDS-PAGE), high purity
SynonymsC1 esterase inhibitor | C1 Inh | C1Inh | C1-inhibiting factor | Serpin G1 | C1 esterase inhibitor | C1 Inh | C1 inhibiting factor | C1 inhibitor | C1-inhibiting factor | C1IN | C1Inh | C1NH | complement component 1 inhibitor | esterase inhibitor | HAE1 |
GradeActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free
Product Description

Purity
≥95% (SDS-PAGE)
Endotoxin level
<0.1 EU/μg
Function
Activation of the C1 complex is under control of the C1-inhibitor. It forms a proteolytically inactive stoichiometric complex with the C1r or C1s proteases. May play a potentially crucial role in regulating important physiological pathways including complement activation, blood coagulation, fibrinolysis and the generation of kinins. Very efficient inhibitor of FXIIa. Inhibits chymotrypsin and kallikrein.

Specifications & PurityActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥95%(SDS-PAGE)
Purity≥95% (SDS-PAGE)
BioactivityMeasured by its ability to inhibit Recombinant Human Complement Component C1s cleavage of a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-K-SBzl). The IC50 is <5 nM, as measured under the described conditions.
Endotoxin Concentration<0.1 EU/μg
Expression SystemCHO
SpeciesHuman
Amino Acids23-500 aa
SequenceNPNATSSSSQDPESLQDRGEGKVATTVISKMLFVEPILEVSSLPTTNSTTNSATKITANTTDEPTTQPTTEPTTQPTIQPTQPTTQLPTDSPTQPTTGSFCPGPVTLCSDLESHSTEAVLGDALVDFSLKLYHAFSAMKKVETNMAFSPFSIASLLTQVLLGAGENTKTNLESILSYPKDFTCVHQALKGFTTKGVTSVSQIFHSPDLAIRDTFVNASRTLYSSSPRVLSNNSDANLELINTWVAKNTNNKISRLLDSLPSDTRLVLLNAIYLSAKWKTTFDPKKTRMEPFHFKNSVIKVPMMNSKKYPVAHFIDQTLKAKVGQLQLSHNLSLVILVPQNLKHRLEDMEQALSPSVFKAIMEKLEMSKFQPTLLTLPRIKVTTSQDMLSIMEKLEFFDFSYDLNLCGLTEDPDLQVSAMQHQTVLELTETGVEAAAASAISVARTLLVFEVQQPFLFVLWDQQHKFPVFMGRVYDPRAHHHHHH
Protein TagC-His
Accession #P05155
Predicted molecular weight54 kDa
SDS-PAGE90 - 110 kDa, under reducing conditions; 100 -120 kDa, under non-reducing conditions.

Product Specifications

FormLyophilized
ReconstitutionReconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -20~-80℃ for more than 1 year. Upon delivery aliquot. Avoid freeze/thaw cycle.

Certificates

Certificate of Analysis(COA)

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Related Documents

 SDS

 Specification Sheet

References

1. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K et al..  (2004)  Complete sequencing and characterization of 21,243 full-length human cDNAs..  Nat Genet,  36  (1): (40-5).  [PMID:14702039] [10.1021/op500134e]
2. Bock SC, Skriver K, Nielsen E, Thøgersen HC, Wiman B, Donaldson VH, Eddy RL, Marrinan J, Radziejewska E, Huber R et al..  (1986)  Human C1 inhibitor: primary structure, cDNA cloning, and chromosomal localization..  Biochemistry,  25  (15): (4292-301).  [PMID:3756141] [10.1021/op500134e]
3. Todd D Taylor,Hideki Noguchi,Yasushi Totoki,Atsushi Toyoda,Yoko Kuroki,Ken Dewar,Christine Lloyd,Takehiko Itoh,Tadayuki Takeda,Dae-Won Kim,Xinwei She,Karen F Barlow,Toby Bloom,Elspeth Bruford,Jean L Chang,Christina A Cuomo,Evan Eichler,Michael G FitzGerald,David B Jaffe,Kurt LaButti,Robert Nicol,Hong-Seog Park,Christopher Seaman,Carrie Sougnez,Xiaoping Yang,Andrew R Zimmer,Michael C Zody,Bruce W Birren,Chad Nusbaum,Asao Fujiyama,Masahira Hattori,Jane Rogers,Eric S Lander,Yoshiyuki Sakaki.  (2006-03-24)  Human chromosome 11 DNA sequence and analysis including novel gene identification..  Nature,  440  ((7083)): (497-500).  [PMID:16554811]
4. Davis, A E AE and 9 more authors..  (1992)  C1 inhibitor hinge region mutations produce dysfunction by different mechanisms..  Nature genetics,      [PMID:1363816]
5. Frangi, D D, Aulak, K S KS, Cicardi, M M, Harrison, R A RA and Davis, A E AE..  (1992)  A dysfunctional C1 inhibitor protein with a new reactive center mutation (Arg-444-->Leu)..  FEBS letters,    (13):   [PMID:1451784]
6. Carter, P E PE, Duponchel, C C, Tosi, M M and Fothergill, J E JE..  (1991)  Complete nucleotide sequence of the gene for human C1 inhibitor with an unusually high density of Alu elements..  European journal of biochemistry,    (23):   [PMID:2026152]
7. Parad, R B RB, Kramer, J J, Strunk, R C RC, Rosen, F S FS and Davis, A E AE..  (1990)  Dysfunctional C1 inhibitor Ta: deletion of Lys-251 results in acquisition of an N-glycosylation site..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:2118657]
8. Stoppa-Lyonnet, D D, Carter, P E PE, Meo, T T and Tosi, M M..  (1990)  Clusters of intragenic Alu repeats predispose the human C1 inhibitor locus to deleterious rearrangements..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:2154751]
9. Levy, N J NJ, Ramesh, N N, Cicardi, M M, Harrison, R A RA and Davis, A E AE..  (1990)  Type II hereditary angioneurotic edema that may result from a single nucleotide change in the codon for alanine-436 in the C1 inhibitor gene..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:2296585]
10. Aulak, K S KS, Cicardi, M M and Harrison, R A RA..  (1990)  Identification of a new P1 residue mutation (444Arg----Ser) in a dysfunctional C1 inhibitor protein contained in a type II hereditary angioedema plasma..  FEBS letters,    (18):   [PMID:2365061]
11. Tosi, M M, Duponchel, C C, Bourgarel, P P, Colomb, M M and Meo, T T..  (1986)  Molecular cloning of human C1 inhibitor: sequence homologies with alpha 1-antitrypsin and other members of the serpins superfamily..  Gene,      [PMID:3089875]
12. Aulak, K S KS and 5 more authors..  (1988)  Dysfunctional C1-inhibitor(At), isolated from a type II hereditary-angio-oedema plasma, contains a P1 'reactive centre' (Arg444----His) mutation..  The Biochemical journal,    (15):   [PMID:3178731]
13. Carter, P E PE, Dunbar, B B and Fothergill, J E JE..  (1988)  Genomic and cDNA cloning of the human C1 inhibitor. Intron-exon junctions and comparison with other serpins..  European journal of biochemistry,    (5):   [PMID:3267220]
14. Rauth, G G, Schumacher, G G, Buckel, P P and Müller-Esterl, W W..  (1988)  Molecular cloning of the cDNA coding for human C1 inhibitor..  Protein sequences & data analysis,      [PMID:3393514]
15. Davis, A E AE and 6 more authors..  (1986)  Human inhibitor of the first component of complement, C1: characterization of cDNA clones and localization of the gene to chromosome 11..  Proceedings of the National Academy of Sciences of the United States of America,      [PMID:3458172]
16. Que, B G BG and Petra, P H PH..  (1986)  Isolation and analysis of a cDNA coding for human C1 inhibitor..  Biochemical and biophysical research communications,    (13):   [PMID:3488058]
17. and Harrison, R A RA..  (1983)  Human C1 inhibitor: improved isolation and preliminary structural characterization..  Biochemistry,    (11):   [PMID:6416294]
18. Stein, P E PE and Carrell, R W RW..  (1995)  What do dysfunctional serpins tell us about molecular mobility and disease?.  Nature structural biology,      [PMID:7749926]
19. Verpy, E E and 6 more authors..  (1995)  Crucial residues in the carboxy-terminal end of C1 inhibitor revealed by pathogenic mutants impaired in secretion or function..  The Journal of clinical investigation,      [PMID:7814636]
20. Zahedi, R R, Bissler, J J JJ, Davis, A E AE, Andreadis, C C and Wisnieski, J J JJ..  (1995)  Unique C1 inhibitor dysfunction in a kindred without angioedema. II. Identification of an Ala443-->Val substitution and functional analysis of the recombinant mutant protein..  The Journal of clinical investigation,      [PMID:7883978]
21. Davis, A E AE, Bissler, J J JJ and Cicardi, M M..  (1993)  Mutations in the C1 inhibitor gene that result in hereditary angioneurotic edema..  Behring Institute Mitteilungen,      [PMID:8172583]
22. Ocejo-Vinyals, J G JG, Leyva-Cobián, F F and Fernández-Luna, J L JL..  (1995)  A mutation unique in serine protease inhibitors (serpins) identified in a family with type II hereditary angioneurotic edema..  Molecular medicine (Cambridge, Mass.),      [PMID:8529136]
23. Verpy, E E and 5 more authors..  (1996)  Exhaustive mutation scanning by fluorescence-assisted mismatch analysis discloses new genotype-phenotype correlations in angiodema..  American journal of human genetics,      [PMID:8755917]
24. Matsushita, M M, Thiel, S S, Jensenius, J C JC, Terai, I I and Fujita, T T..  (2000)  Proteolytic activities of two types of mannose-binding lectin-associated serine protease..  Journal of immunology (Baltimore, Md. : 1950),    (1):   [PMID:10946292]
25. Lathem, Wyndham W WW and 6 more authors..  (2002)  StcE, a metalloprotease secreted by Escherichia coli O157:H7, specifically cleaves C1 esterase inhibitor..  Molecular microbiology,      [PMID:12123444]
26. Zhang, Hui H, Li, Xiao-Jun XJ, Martin, Daniel B DB and Aebersold, Ruedi R..  (2003)  Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry..  Nature biotechnology,      [PMID:12754519]
27. Bos, Ineke G A IG and 5 more authors..  (2003)  The functional integrity of the serpin domain of C1-inhibitor depends on the unique N-terminal domain, as revealed by a pathological mutant..  The Journal of biological chemistry,    (8):   [PMID:12773530]
28. Kalmár, Lajos L and 7 more authors..  (2003)  Mutation screening of the C1 inhibitor gene among Hungarian patients with hereditary angioedema..  Human mutation,      [PMID:14635117]
29. Bunkenborg, Jakob J, Pilch, Bartosz J BJ, Podtelejnikov, Alexandre V AV and Wiśniewski, Jacek R JR..  (2004)  Screening for N-glycosylated proteins by liquid chromatography mass spectrometry..  Proteomics,      [PMID:14760718]
30. Lathem, Wyndham W WW, Bergsbaken, Tessa T and Welch, Rodney A RA..  (2004)  Potentiation of C1 esterase inhibitor by StcE, a metalloprotease secreted by Escherichia coli O157:H7..  The Journal of experimental medicine,    (19):   [PMID:15096536]
31. Liu, Tao T and 6 more authors..  ()  Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry..  Journal of proteome research,      [PMID:16335952]
32. Kang, H R HR and 7 more authors..  (2006)  Normal C1 inhibitor mRNA expression level in type I hereditary angioedema patients: newly found C1 inhibitor gene mutations..  Allergy,      [PMID:16409206]
33. Beinrohr, László L and 5 more authors..  (2007)  C1 inhibitor serpin domain structure reveals the likely mechanism of heparin potentiation and conformational disease..  The Journal of biological chemistry,    (20):   [PMID:17488724]
34. Xu, Y-Y YY and 8 more authors..  (2012)  Mutational spectrum and geno-phenotype correlation in Chinese families with hereditary angioedema..  Allergy,      [PMID:22994404]
35. Bian, Yangyang Y and 9 more authors..  (2014)  An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome..  Journal of proteomics,    (16):   [PMID:24275569]
36. Bafunno, Valeria V and 8 more authors..  (2014)  Mutational spectrum of the c1 inhibitor gene in a cohort of Italian patients with hereditary angioedema: description of nine novel mutations..  Annals of human genetics,      [PMID:24456027]

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