Recombinant Human SFRP4 Protein, >95%(SDS-PAGE), high purity

Features and benefits
  • Expression System: CHO
  • Accession #: Q6FHJ7
  • Protein Tag: C-His
  • Bioactivity: Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T 1/2 2A6 mouse embryonal fibroblast cells. The ED50 for this effect is typically 2-20 μg/mL in the presence of 20 ng/mL of Wnt3a.
  • Endotoxin Concentration: <1.0 EU/μg
Item Number
rp151482
Grouped product items
SKUSizeAvailabilityPrice Qty
rp151482-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$129.90
rp151482-50μg
50μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$399.90
rp151482-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$769.90
rp151482-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$3,079.90

Animal Free, >95%(SDS-PAGE), Active, CHO Stable Cells, C-His tag, 22-346 aa

Basic Description

Product NameRecombinant Human SFRP4 Protein, >95%(SDS-PAGE), high purity
SynonymsFrizzled protein | Frizzled protein human endometrium | FRP 4 | FRP4 | FrpHE | human endometrium | PYL | Secreted frizzled-related protein 4 | sFRP-4 | Sfrp4 | SFRP4_HUMAN | DDC-4 | Frizzled protein, human endometrium | FRP-4 | FRP-AP | FRPHEMGC26498 | Fr
GradeActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance
Product Description

Purity
>95% SDS-PAGE.
Function
Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling (By similarity). Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake.

Specifications & PurityActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE)
Purity>95%(SDS-PAGE)
BioactivityMeasured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T 1/2 2A6 mouse embryonal fibroblast cells. The ED50 for this effect is typically 2-20 μg/mL in the presence of 20 ng/mL of Wnt3a.
Endotoxin Concentration<1.0 EU/μg
Expression SystemCHO
SpeciesHuman
Amino Acids22-346 aa
SequenceAPCEAVRIPMCRHMPWNITRMPNHLHHSTQENAILAIEQYEELVDVNCSAVLRFFLCAMYAPICTLEFLHDPIKPCKSVCQRARDDCEPLMKMYNHSWPESLACDELPVYDRGVCISPEAIVTDLPEDVKWIDITPDMMVQERPLDVDCKRLSPDRCKCKKVKPTLATYLSKNYSYVIHAKIKAVQRSGCNEVTTVVDVKEIFKSSSPIPRTQVPLITNSSCQCPHILPHQDVLIMCYEWRSRMMLLENCLVEKWRDQLSKRSIQWEERLQEQRRTVQDKKKTAGRTSRSNPPKPKGKPPAPKPASPKKNIKTRSAQKRTNPKRVAHHHHHHHHHH
Protein TagC-His
Accession #Q6FHJ7
Predicted molecular weight39 kDa
SDS-PAGE60.0 kDa, under reducing conditions; 57.0 kDa, under non-reducing conditions.

Images

Recombinant Human SFRP4 Protein (rp151482) - Protein Bioactivity
Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T 1/2 2A6 mouse embryonal fibroblast cells. The ED₅₀ for this effect is typically 2 - 20 μg/mL in the presence of 20 ng/mL of Wnt3a.

Recombinant Human SFRP4 Protein (rp151482) - SDS-PAGE
3 μg/lane of Recombinant Human SFRP4 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 60.0 kDa under reducing conditions and 57.0 kDa under non-reducing conditions.

Product Specifications

FormLyophilized
ReconstitutionIt is recommended that sterile water be added to the vial to prepare a stock solution.
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageSamples are stable for twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage.

Certificates

C of A & Other Certificates

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ23F1001334Certificate of AnalysisOct 19, 2023 rp151482
ZJ23F1001333Certificate of AnalysisOct 19, 2023 rp151482
ZJ23F1001332Certificate of AnalysisOct 19, 2023 rp151482

Related Documents

References

1. Scherer SW, Cheung J, MacDonald JR, Osborne LR, Nakabayashi K, Herbrick JA, Carson AR, Parker-Katiraee L, Skaug J, Khaja R et al..  (2003)  Human chromosome 7: DNA sequence and biology..  Science,  300  (5620): (767-72).  [PMID:12690205] [10.1021/op500134e]
2. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K et al..  (2004)  Complete sequencing and characterization of 21,243 full-length human cDNAs..  Nat Genet,  36  (1): (40-5).  [PMID:14702039] [10.1021/op500134e]
3. Abu-Jawdeh, G G and 6 more authors..  (1999)  Differential expression of frpHE: a novel human stromal protein of the secreted frizzled gene family, during the endometrial cycle and malignancy..  Laboratory investigation; a journal of technical methods and pathology,      [PMID:10211996]
4. Schumann, H H, Holtz, J J, Zerkowski, H R HR and Hatzfeld, M M..  (2000)  Expression of secreted frizzled related proteins 3 and 4 in human ventricular myocardium correlates with apoptosis related gene expression..  Cardiovascular research,      [PMID:10728394]
5. Berndt, Theresa T and 8 more authors..  (2003)  Secreted frizzled-related protein 4 is a potent tumor-derived phosphaturic agent..  The Journal of clinical investigation,      [PMID:12952927]
6. Drake, J J and 6 more authors..  (2009)  Expression of secreted frizzled-related protein 4 (SFRP4) in primary serous ovarian tumours..  European journal of gynaecological oncology,      [PMID:19480240]
7. Bukhari, Shazia Anwer SA, Shamshari, Waseem Akhtar WA, Ur-Rahman, Mahmood M, Zia-Ul-Haq, Muhammad M and Jaafar, Hawa Z E HZ..  (2014)  Computer aided screening of secreted frizzled-related protein 4 (SFRP4): a potential control for diabetes mellitus..  Molecules (Basel, Switzerland),    (11):   [PMID:25019556]
8. Kiper, Pelin O Simsek POS and 23 more authors..  (2016)  Cortical-Bone Fragility--Insights from sFRP4 Deficiency in Pyle's Disease..  The New England journal of medicine,    (30):   [PMID:27355534]

Solution Calculators