| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| rp152522-5μg | 5μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $59.90 | |
| rp152522-20μg | 20μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $139.90 | |
| rp152522-100μg | 100μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $449.90 | |
| rp152522-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,569.90 |
Carrier Free, >95%(SDS-PAGE), Active, E.coli, His&GST, 298-441aa
| Product Name | Recombinant Human TNF RII/TNFRSF1B Protein, ＞95%(SDS-PAGE), high purityused for Cell culture, Activity Assays, ELISA, SDS-PAGE various applications |
|---|---|
| Synonyms | CD120b antigen | CD120b | Etanercept | p75 TNF receptor | p75TBPII | p75TNFR | soluble TNFR1B variant 1 | TNF RII | TNFBRp80 TNF-alpha receptor | TNF-R2 | TNFR2TNFR1B | TNF-R75 | TNFR80 | TNFRII | TNF-RII | TNF-R-II | TNFR-II | TNFRSF1B | tumor necrosis f |
| Grade | ActiBioPure™, Azide Free, Bioactive, Carrier Free, Low Endotoxin |
| Product Description | Purity >95% SDS-PAGE. Function Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity. |
| Specifications & Purity | ActiBioPure™, Bioactive, Carrier Free, Low Endotoxin, Azide Free, ≥95%(SDS-PAGE) |
| Purity | ＞95%(SDS-PAGE) |
| Bioactivity | The binding activity of TNFRSF1B and TNFa, the EC50 was 0.0470~0.085 μg/ml. |
| Endotoxin Concentration | <1.0 EU/mg |
| Expression System | E. coli |
| Species | Human |
| Amino Acids | 298-441 aa |
| Sequence | MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDGSTSGSGHHHHHHSAGLVPRGSTAIGMKETAAAKFERQHMDSPDLGTGGGSGIEGRGSMGYRGSEFAKVPHLPADKARGTQGPEQQHLLITAPSSSSSSLESSASALDRRAPTRNQPQAPGVEASGAGEARASTGSSDSSPGGHGTQVNVTCIVNVCSSSDHSSQCSSQASSTMGDTDSSPSESPKDEQVPFSKEECAFRSQLETPETLL |
| Protein Tag | N-His & GST |
| Protein Length | Protein fragment |
| Accession # | P20333 |
| Source | Recombinant |
| Predicted molecular weight | 44.8 kDa |
Recombinant Human TNF RII/TNFRSF1B Protein (rp152522)-Protein Bioactivity
Measured by its binding ability in a functional ELISA.Tumor Necrosis Factor Alpha (TNF-α) has been identified as an interactor of TNFRSF1B, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human TNFRSF1B and recombinant human TNF-α.
Recombinant Human TNF RII/TNFRSF1B Protein (rp152522)-SDS-PAGE
3μg/lane of Recombinant Human VCAM1 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 51.3 kDa under reducing conditions and 49 kDa under non-reducing conditions.
| Application | Cell culture, Activity Assays, ELISA, SDS-PAGE |
|---|---|
| Form | Lyophilized |
| Reconstitution | Reconstitute in ddH2O to a concentration ≤0.1mg/mL. Do not vortex. |
| Storage Temp | Store at -80°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Avoid repeated freeze/thaw cycles. Store at 2-8℃ for one month. Aliquot and store at -80℃ for 12 months. |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0600460 | Certificate of Analysis | Jun 30, 2023 | rp152522 |
| ZJ23F0600461 | Certificate of Analysis | Jun 30, 2023 | rp152522 |
| ZJ23F0600462 | Certificate of Analysis | Jun 30, 2023 | rp152522 |
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