Recombinant Human TRAIL R2/TNFRSF10B Protein, >97% (SDS-PAGE&HPLC), high purity

Features and benefits
  • Expression System: E.coli
  • Protein Tag: No tag
  • Bioactivity: Fully biologically active when compared to standard. rHusTRAIL-R2 reduced the production of LPS-induced TNF by its ability to neutralize endogenous TRAIL in fresh human PBMC. In this assay, endogenous TRAIL is induced during a 24 hour exposure to LPS (10
  • Endotoxin Concentration: <1.0 EU/μg
Item Number
rp145269
Grouped product items
SKUSizeAvailabilityPrice Qty
rp145269-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$59.90
rp145269-50μg
50μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$139.90
rp145269-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$239.90
rp145269-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,989.90

Animal Free, >97% (SDS-PAGE&HPLC), Active, E.coli, No tag, 52-183 aa

Basic Description

Product NameRecombinant Human TRAIL R2/TNFRSF10B Protein, >97% (SDS-PAGE&HPLC), high purity
SynonymsCD262 antigen | CD262 | death domain containing receptor for TRAIL/Apo-2L | Death receptor 5 | DR5 | DR5TRICK2B | Fas-like protein | KILLER/DR5 | KILLERapoptosis inducing protein TRICK2A/2B | TNF-related apoptosis-inducing ligand receptor 2 | TNFRSF10B |
GradeActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free
Product Description

Purity
>97% (SDS-PAGE&HPLC)
Endotoxin level
<1.0 EU/μg
Function
Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis.

Specifications & PurityActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥97%(SDS-PAGE&HPLC)
Purity>97% (SDS-PAGE&HPLC)
BioactivityFully biologically active when compared to standard. rHusTRAIL-R2 reduced the production of LPS-induced TNF by its ability to neutralize endogenous TRAIL in fresh human PBMC. In this assay, endogenous TRAIL is induced during a 24 hour exposure to LPS (10
Endotoxin Concentration<1.0 EU/μg
Expression SystemE.coli
SpeciesHuman
Amino Acids52-183 aa
SequenceESALITQQDLAPQQRAAPQQKRSSPSEGLCPPGHHISEDGRDCISCKYGQDYSTHWNDLLFCLRCTRCDSGEVELSPCTTTRNTVCQCEEGTFREEDSPEMCRKCRTGCPRGMVKVGDCTPWSDIECVHKES
Protein TagNo tag
Predicted molecular weight14.8 kDa
SDS-PAGE14.8 kDa

Images

Recombinant Human TRAIL R2/TNFRSF10B Protein (rp145269) - SDS-PAGE
Recombinant Human TRAIL R2/TNFRSF10B Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining. Showing a single band at 16.3 kDa.

Product Specifications

FormLyophilized
ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportio
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And Storage12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution. Upon delivery aliquot. Avoid freeze/thaw cycle.

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ23F1001340Certificate of AnalysisOct 19, 2023 rp145269
ZJ23F1001341Certificate of AnalysisOct 19, 2023 rp145269
ZJ23F1001342Certificate of AnalysisOct 19, 2023 rp145269

Related Documents

References

1. Clark HF, Gurney AL, Abaya E, Baker K, Baldwin D, Brush J, Chen J, Chow B, Chui C, Crowley C et al..  (2003)  The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment..  Genome Res,  13  (10): (2265-70).  [PMID:12975309] [10.1021/op500134e]
2. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P et al..  (2004)  The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)..  Genome Res,  14  (10B): (2121-7).  [PMID:15489334] [10.1021/op500134e]
3. Ohtsuki T, Kikuchi H, Koyano T, Kowithayakorn T, Sakai T, Ishibashi M..  (2009)  Death receptor 5 promoter-enhancing compounds isolated from Catimbium speciosum and their enhancement effect on TRAIL-induced apoptosis..  Bioorg Med Chem,  17  (18): (6748-6754).  [PMID:19682913] [10.1016/j.bmc.2009.07.041]
4. Kikuchi H, Ohtsuki T, Koyano T, Kowithayakorn T, Sakai T, Ishibashi M..  (2010)  Activity of mangosteen xanthones and teleocidin a-2 in death receptor expression enhancement and tumor necrosis factor related apoptosis-inducing ligand assays..  J Nat Prod,  73  (3): (452-455).  [PMID:19788289] [10.1021/np900404e]
5. Pan, G G and 5 more authors..  (1997)  An antagonist decoy receptor and a death domain-containing receptor for TRAIL..  Science (New York, N.Y.),    (8):   [PMID:9242610]
6. Sheridan, J P JP and 12 more authors..  (1997)  Control of TRAIL-induced apoptosis by a family of signaling and decoy receptors..  Science (New York, N.Y.),    (8):   [PMID:9242611]
7. Screaton, G R GR and 5 more authors..  (1997)  TRICK2, a new alternatively spliced receptor that transduces the cytotoxic signal from TRAIL..  Current biology : CB,    (1):   [PMID:9285725]
8. Walczak, H H and 11 more authors..  (1997)  TRAIL-R2: a novel apoptosis-mediating receptor for TRAIL..  The EMBO journal,    (1):   [PMID:9311998]
9. MacFarlane, M M and 5 more authors..  (1997)  Identification and molecular cloning of two novel receptors for the cytotoxic ligand TRAIL..  The Journal of biological chemistry,    (10):   [PMID:9325248]
10. Wu, G S GS and 14 more authors..  (1997)  KILLER/DR5 is a DNA damage-inducible p53-regulated death receptor gene..  Nature genetics,      [PMID:9326928]
11. Schneider, P P and 5 more authors..  (1997)  Characterization of two receptors for TRAIL..  FEBS letters,    (27):   [PMID:9373179]
12. Chaudhary, P M PM and 5 more authors..  (1997)  Death receptor 5, a new member of the TNFR family, and DR4 induce FADD-dependent apoptosis and activate the NF-kappaB pathway..  Immunity,      [PMID:9430227]
13. Arai, T T and 5 more authors..  (1998)  Genomic organization and mutation analyses of the DR5/TRAIL receptor 2 gene in colorectal carcinomas..  Cancer letters,    (27):   [PMID:10072170]
14. Mongkolsapaya, J J and 6 more authors..  (1999)  Structure of the TRAIL-DR5 complex reveals mechanisms conferring specificity in apoptotic initiation..  Nature structural biology,      [PMID:10542098]
15. Hymowitz, S G SG and 7 more authors..  (1999)  Triggering cell death: the crystal structure of Apo2L/TRAIL in a complex with death receptor 5..  Molecular cell,      [PMID:10549288]
16. Hillman, R Tyler RT, Green, Richard E RE and Brenner, Steven E SE..  (2004)  An unappreciated role for RNA surveillance..  Genome biology,      [PMID:14759258]
17. Yamaguchi, Hirohito H and Wang, Hong-Gang HG..  (2004)  CHOP is involved in endoplasmic reticulum stress-induced apoptosis by enhancing DR5 expression in human carcinoma cells..  The Journal of biological chemistry,    (29):   [PMID:15322075]
18. Nusbaum, Chad C and 75 more authors..  (2006)  DNA sequence and analysis of human chromosome 8..  Nature,    (19):   [PMID:16421571]
19. Maddipatla, Sreeram S, Hernandez-Ilizaliturri, Francisco J FJ, Knight, Joy J and Czuczman, Myron S MS..  (2007)  Augmented antitumor activity against B-cell lymphoma by a combination of monoclonal antibodies targeting TRAIL-R1 and CD20..  Clinical cancer research : an official journal of the American Association for Cancer Research,    (1):   [PMID:17671142]
20. Bechtel, Stephanie S and 18 more authors..  (2007)  The full-ORF clone resource of the German cDNA Consortium..  BMC genomics,    (31):   [PMID:17974005]
21. Sun, M M, Song, L L, Li, Y Y, Zhou, T T and Jope, R S RS..  (2008)  Identification of an antiapoptotic protein complex at death receptors..  Cell death and differentiation,      [PMID:18846110]
22. Smith, Wendell W and 17 more authors..  (2013)  Human cytomegalovirus glycoprotein UL141 targets the TRAIL death receptors to thwart host innate antiviral defenses..  Cell host & microbe,    (13):   [PMID:23498957]
23. Nemčovičová, Ivana; Benedict, Chris A and Zajonc, Dirk M..  (2013)  Structure of human cytomegalovirus UL141 binding to TRAIL-R2 reveals novel, non-canonical death receptor interactions..  PLoS pathogens,      [PMID:23555243]
24. Newson, Joshua P M and 11 more authors..  (2019)  Salmonella Effectors SseK1 and SseK3 Target Death Domain Proteins in the TNF and TRAIL Signaling Pathways..  Molecular & cellular proteomics : MCP,      [PMID:30902834]

Solution Calculators