| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| rp152656-10μg | 10μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $87.90 | |
| rp152656-50μg | 50μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $327.90 | |
| rp152656-100μg | 100μg | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $597.90 | |
| rp152656-1mg | 1mg | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $3,597.90 |
Animal Free, >97%(SDS-PAGE and HPLC), Active, E.coli, No tag, 22-80 aa
| Product Name | Recombinant Human Trefoil Factor 3 Protein, ＞97%(SDS-PAGE and HPLC), high purity |
|---|---|
| Synonyms | hITF;hP1.B;Intestinal trefoil factor; ITF; mITF; OTTMUSP00000021729; P1B; Polypeptide P1.B; TFF3; TFF3_HUMAN; TFI; TREFOIL; Trefoil factor (intestinal); Trefoil factor 3 |
| Grade | ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance |
| Product Description | Purity >97% by SDS-PAGE and HPLC analyses. Function Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen). |
| Specifications & Purity | ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥97%(SDS-PAGE&HPLC) |
| Purity | ＞97%(SDS-PAGE and HPLC) |
| Bioactivity | Fully biologically active when compared to standard. The ED₅₀ as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10 μg/ml, corresponding to a specific activity of > 100 IU/mg. |
| Endotoxin Concentration | ＜1.0 EU/μg |
| Expression System | E.coli |
| Species | Human |
| Amino Acids | 22-80 aa |
| Sequence | EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK PLQEAECTF |
| Protein Tag | No tag |
| Protein Length | Full length protein |
| Accession # | Q07654 |
| Source | Recombinant |
| Predicted molecular weight | 13.2 kDa |
| SDS-PAGE | 13.2 kDa |
Recombinant Human Trefoil Factor 3 Protein (rp152656)-Protein Bioactivity
Fully biologically active when compared to standard. The ED₅₀ as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10 μg/mL, corresponding to a specific activity of > 100 IU/mg.
Recombinant Human Trefoil Factor 3 Protein (rp152656)-SDS-PAGE
Recombinant Human Trefoil Factor 3 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining. Showing a single band at 14.5 kDa under reducing conditions and 29 kDa under non-reducing conditions.
| Form | Lyophilized |
|---|---|
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportio |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconst |
Enter Lot Number to search for COA:
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F0600356 | Certificate of Analysis | Mar 26, 2024 | rp152656 |
| ZJ23F0600357 | Certificate of Analysis | Mar 26, 2024 | rp152656 |
| ZJ23F0600358 | Certificate of Analysis | Mar 26, 2024 | rp152656 |
| 1. Hauser, F F and 6 more authors.. (1993) hP1.B, a human P-domain peptide homologous with rat intestinal trefoil factor, is expressed also in the ulcer-associated cell lineage and the uterus.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:8346203] |
| 2. Podolsky, D K DK and 7 more authors.. (1993) Identification of human intestinal trefoil factor. Goblet cell-specific expression of a peptide targeted for apical secretion.. The Journal of biological chemistry, (25): [PMID:8454642] |
| 3. Seib, T T and 8 more authors.. (1997) The three human trefoil genes TFF1, TFF2, and TFF3 are located within a region of 55 kb on chromosome 21q22.3.. Genomics, (15): [PMID:9070946] |
| 4. Hattori, M M and 64 more authors.. (2000) The DNA sequence of human chromosome 21.. Nature, (18): [PMID:10830953] |
| 5. Berry, A A and 16 more authors.. (2000) Refined localization of autosomal recessive nonsyndromic deafness DFNB10 locus using 34 novel microsatellite markers, genomic structure, and exclusion of six known genes in the region.. Genomics, (15): [PMID:10950923] |
| 6. Oertel, M M and 5 more authors.. (2001) Trefoil factor family-peptides promote migration of human bronchial epithelial cells: synergistic effect with epidermal growth factor.. American journal of respiratory cell and molecular biology, [PMID:11694446] |
| 7. Casadei, Raffaella R and 10 more authors.. (2003) mRNA 5' region sequence incompleteness: a potential source of systematic errors in translation initiation codon assignment in human mRNAs.. Gene, (4): [PMID:14637006] |
| 8. Muskett, Frederick W FW, May, Felicity E B FE, Westley, Bruce R BR and Feeney, James J.. (2003) Solution structure of the disulfide-linked dimer of human intestinal trefoil factor (TFF3): the intermolecular orientation and interactions are markedly different from those of other dimeric trefoil proteins.. Biochemistry, (30): [PMID:14690424] |
| 9. Zhang, Zemin Z and Henzel, William J WJ.. (2004) Signal peptide prediction based on analysis of experimentally verified cleavage sites.. Protein science : a publication of the Protein Society, [PMID:15340161] |
