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Recombinant Human Tyro3/Dtk Protein, ≥95% (SDS-PAGE), high purity

  • ActiBioPure™
  • Animal Free
  • Azide Free
  • Bioactive
  • Carrier Free
  • ≥95%(SDS-PAGE)
Features and benefits
  • Expression System: HEK293
  • Accession #: Q06418
  • Protein Tag: C-hFc & His
  • Bioactivity: Immobilized Human Gas6 at 2 μg/mL (100 μL/well) can bind Human TYRO3 with a linear range of 0.1-5.6 ng/mL.
  • Endotoxin Concentration: <0.1 EU/μg
Item Number
rp152849
Grouped product items
SKUSizeAvailabilityPrice Qty
rp152849-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$59.90
rp152849-50μg
50μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$269.90
rp152849-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$409.90
rp152849-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,499.90

Animal Free, ≥95% (SDS-PAGE), Active, HEK293, C-hFc&His tag, 41-428 aa

Basic Description

Product NameRecombinant Human Tyro3/Dtk Protein, ≥95% (SDS-PAGE), high purity
SynonymsBrt; BYK; DTK; Etk-2; protein tyrosine kinase 3; Rek; RSE; SKY; Tif; tyro3; TYRO3 protein tyrosine kinase; TYRO3_HUMAN; Tyrosine-protein kinase byk; Tyrosine-protein kinase DTK; Tyrosine-protein kinase receptor TYRO3; Tyrosine-protein kinase RSE; Tyrosine
GradeActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free
Product Description

Purity
≥95% (SDS-PAGE)
Endotoxin level
<0.1 EU/μg
Function
May be involved in cell adhesion processes, particularly in the central nervous system. In case of filovirus infection, seems to function as a cell entry factor.

Specifications & PurityActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥95%(SDS-PAGE)
Purity≥95% (SDS-PAGE)
BioactivityImmobilized Human Gas6 at 2 μg/mL (100 μL/well) can bind Human TYRO3 with a linear range of 0.1-5.6 ng/mL.
Endotoxin Concentration<0.1 EU/μg
Expression SystemHEK293
SpeciesHuman
Amino Acids41-428 aa
SequenceAGLKLMGAPVKLTVSQGQPVKLNCSVEGMEEPDIQWVKDGAVVQNLDQLYIPVSEQHWIGFLSLKSVERSDAGRYWCQVEDGGETEISQPVWLTVEGVPFFTVEPKDLAVPPNAPFQLSCEAVGPPEPVTIVWWRGTTKIGGPAPSPSVLNVTGVTQSTMFSCEAHNLKGLASSRTATVHLQALPAAPFNITVTKLSSSNASVAWMPGADGRALLQSCTVQVTQAPGGWEVLAVVVPVPPFTCLLRDLVPATNYS
Protein TagC-hFc & His
Accession #Q06418
Predicted molecular weight70 kDa
SDS-PAGE90-110 kDa, under reducing conditions

Images

Recombinant Human Tyro3/Dtk Protein (rp152849) - Protein Bioactivity
Immobilized Human Gas6 at 2.0 μg/mL can bind Recombinant Human Tyro3/Dtk Protein (rp152849) with a linear range of 0.1 - 4.5 ng/mL. The EC₅₀ for this effect is 5.1 ng/mL.

Recombinant Human Tyro3/Dtk Protein (rp152849) - SDS-PAGE
3 μg/lane of Recombinant Human Tyro3/Dtk Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 90 - 110 kDa under reducing conditions and 90 - 110 & 180 - 220 kDa under non-reducing conditions.

Product Specifications

FormLyophilized
ReconstitutionReconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -20~-80℃ for more than 1 year. Upon delivery aliquot. Avoid freeze/thaw cycle.

Certificates

Certificate of Analysis(COA)

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3 results found

Lot NumberCertificate TypeDateItem
ZJ24F0707625Certificate of AnalysisJul 03, 2024 rp152849
ZJ24F0707624Certificate of AnalysisJul 03, 2024 rp152849
ZJ24F0707623Certificate of AnalysisJul 03, 2024 rp152849

Related Documents

References

1. Stitt TN, Conn G, Gore M, Lai C, Bruno J, Radziejewski C, Mattsson K, Fisher J, Gies DR, Jones PF.  (1995)  The anticoagulation factor protein S and its relative, Gas6, are ligands for the Tyro 3/Axl family of receptor tyrosine kinases..  Cell,  80  (4): (661-70).  [PMID:7867073]
2. Nagata K, Ohashi K, Nakano T, Arita H, Zong C, Hanafusa H, Mizuno K.  (1996)  Identification of the product of growth arrest-specific gene 6 as a common ligand for Axl, Sky, and Mer receptor tyrosine kinases..  J Biol Chem,  271  (47): (30022-7).  [PMID:8939948]
3. Heiring C, Dahlbäck B, Muller YA.  (2004)  Ligand recognition and homophilic interactions in Tyro3: structural insights into the Axl/Tyro3 receptor tyrosine kinase family..  J Biol Chem,  279  (8): (6952-8).  [PMID:14623883]
4. Mollard A, Warner SL, Call LT, Wade ML, Bearss JJ, Verma A, Sharma S, Vankayalapati H, Bearss DJ.  (2011)  Design, Synthesis and Biological Evaluation of a Series of Novel Axl Kinase Inhibitors..  ACS Med Chem Lett,  (12): (907-912).  [PMID:22247788]
5. Okuzumi T, Fiedler D, Zhang C, Gray DC, Aizenstein B, Hoffman R, Shokat KM.  (2009)  Inhibitor hijacking of Akt activation..  Nat Chem Biol,  (7): (484-93).  [PMID:19465931]
6. Paolino M, Choidas A, Wallner S, Pranjic B, Uribesalgo I, Loeser S, Jamieson AM, Langdon WY, Ikeda F, Fededa JP et al..  (2014)  The E3 ligase Cbl-b and TAM receptors regulate cancer metastasis via natural killer cells..  Nature,  507  (7493): (508-12).  [PMID:24553136]
7. Hobson AD, Harris CM, van der Kam EL, Turner SC, Abibi A, Aguirre AL, Bousquet P, Kebede T, Konopacki DB, Gintant G et al..  (2015)  Discovery of A-971432, An Orally Bioavailable Selective Sphingosine-1-Phosphate Receptor 5 (S1P5) Agonist for the Potential Treatment of Neurodegenerative Disorders..  J Med Chem,  58  (23): (9154-70).  [PMID:26509640]
8. Weihe Zhang,Dehui Zhang,Michael A Stashko,Deborah DeRyckere,Debra Hunter,Dmitri Kireev,Michael J Miley,Christopher Cummings,Minjung Lee,Jacqueline Norris-Drouin,Wendy M Stewart,Susan Sather,Yingqiu Zhou,Gregory Kirkpatrick,Mischa Machius,William P Janzen,H Shelton Earp,Douglas K Graham,Stephen V Frye,Xiaodong Wang.  (2013-11-08)  Pseudo-cyclization through intramolecular hydrogen bond enables discovery of pyridine substituted pyrimidines as new Mer kinase inhibitors..  Journal of medicinal chemistry,  56  ((23)): (9683-9692).  [PMID:24195762]
9. Emily J Hanan,Charles Eigenbrot,Marian C Bryan,Daniel J Burdick,Bryan K Chan,Yuan Chen,Jennafer Dotson,Robert A Heald,Philip S Jackson,Hank La,Michael D Lainchbury,Shiva Malek,Hans E Purkey,Gabriele Schaefer,Stephen Schmidt,Eileen M Seward,Steve Sideris,Christine Tam,Shumei Wang,Siew Kuen Yeap,Ivana Yen,Jianping Yin,Christine Yu,Inna Zilberleyb,Timothy P Heffron.  (2014-11-11)  Discovery of selective and noncovalent diaminopyrimidine-based inhibitors of epidermal growth factor receptor containing the T790M resistance mutation..  Journal of medicinal chemistry,  57  ((23)): (10176-10191).  [PMID:25383627]
10. McDermott U, Sharma SV, Dowell L, Greninger P, Montagut C, Lamb J, Archibald H, Raudales R, Tam A, Lee D, Rothenberg SM, Supko JG, Sordella R, Ulkus LE, Iafrate AJ, Maheswaran S, Njauw CN, Tsao H, Drew L, Hanke JH, Ma XJ, Erlander MG, Gray NS, Haber DA, Settleman J..  (2007)  Identification of genotype-correlated sensitivity to selective kinase inhibitors by using high-throughput tumor cell line profiling..  Proc Natl Acad Sci U S A,  104  (50): (19936-19941).  [PMID:18077425]
11. Liu J, Yang C, Simpson C, Deryckere D, Van Deusen A, Miley MJ, Kireev D, Norris-Drouin J, Sather S, Hunter D, Korboukh VK, Patel HS, Janzen WP, Machius M, Johnson GL, Earp HS, Graham DK, Frye SV, Wang X..  (2012)  Discovery of Novel Small Molecule Mer Kinase Inhibitors for the Treatment of Pediatric Acute Lymphoblastic Leukemia..  ACS Med Chem Lett,  (2): (129-134).  [PMID:22662287]
12. Lin WH, Hsu JT, Hsieh SY, Chen CT, Song JS, Yen SC, Hsu T, Lu CT, Chen CH, Chou LH, Yang YN, Chiu CH, Chen CP, Tseng YJ, Yen KJ, Yeh CF, Chao YS, Yeh TK, Jiaang WT..  (2013)  Discovery of 3-phenyl-1H-5-pyrazolylamine derivatives containing a urea pharmacophore as potent and efficacious inhibitors of FMS-like tyrosine kinase-3 (FLT3)..  Bioorg Med Chem,  21  (11): (2856-2867).  [PMID:23618709]
13. Zhang W, McIver AL, Stashko MA, DeRyckere D, Branchford BR, Hunter D, Kireev D, Miley MJ, Norris-Drouin J, Stewart WM, Lee M, Sather S, Zhou Y, Di Paola JA, Machius M, Janzen WP, Earp HS, Graham DK, Frye SV, Wang X..  (2013)  Discovery of Mer specific tyrosine kinase inhibitors for the treatment and prevention of thrombosis..  J Med Chem,  56  (23): (9693-9700).  [PMID:24219778]
14. Zhang W, DeRyckere D, Hunter D, Liu J, Stashko MA, Minson KA, Cummings CT, Lee M, Glaros TG, Newton DL, Sather S, Zhang D, Kireev D, Janzen WP, Earp HS, Graham DK, Frye SV, Wang X..  (2014)  UNC2025, a potent and orally bioavailable MER/FLT3 dual inhibitor..  J Med Chem,  57  (16): (7031-7041).  [PMID:25068800]
15. Nakano H, Hasegawa T, Saito N, Furukawa K, Mukaida N, Kojima H, Okabe T, Nagano T..  (2015)  Design and synthesis of an in vivo-efficacious PIM3 kinase inhibitor as a candidate anti-pancreatic cancer agent..  Bioorg Med Chem Lett,  25  (24): (5687-5693).  [PMID:26547690]
16. Daydé-Cazals B, Fauvel B, Singer M, Feneyrolles C, Bestgen B, Gassiot F, Spenlinhauer A, Warnault P, Van Hijfte N, Borjini N, Chevé G, Yasri A..  (2016)  Rational Design, Synthesis, and Biological Evaluation of 7-Azaindole Derivatives as Potent Focused Multi-Targeted Kinase Inhibitors..  J Med Chem,  59  (8): (3886-3905).  [PMID:27010810]
17. Zhu W, Chen H, Wang Y, Wang J, Peng X, Chen X, Gao Y, Li C, He Y, Ai J, Geng M, Zheng M, Liu H..  (2017)  Design, Synthesis, and Pharmacological Evaluation of Novel Multisubstituted Pyridin-3-amine Derivatives as Multitargeted Protein Kinase Inhibitors for the Treatment of Non-Small Cell Lung Cancer..  J Med Chem,  60  (14): (6018-6035).  [PMID:28714692]
18. Fukuda T, Goto R, Kiho T, Ueda K, Muramatsu S, Hashimoto M, Aki A, Watanabe K, Tanaka N..  (2017)  Discovery of DS28120313 as a potent orally active hepcidin production inhibitor: Design and optimization of novel 4,6-disubstituted indazole derivatives..  Bioorg Med Chem Lett,  27  (23): (5252-5257).  [PMID:29079471]
19. Myers SH, Temps C, Houston DR, Brunton VG, Unciti-Broceta A..  (2018)  Development of Potent Inhibitors of Receptor Tyrosine Kinases by Ligand-Based Drug Design and Target-Biased Phenotypic Screening..  J Med Chem,  61  (5): (2104-2110).  [PMID:29466002]
20. Baladi T, Aziz J, Dufour F, Abet V, Stoven V, Radvanyi F, Poyer F, Wu TD, Guerquin-Kern JL, Bernard-Pierrot I, Garrido SM, Piguel S..  (2018)  Design, synthesis, biological evaluation and cellular imaging of imidazo[4,5-b]pyridine derivatives as potent and selective TAM inhibitors..  Bioorg Med Chem,  26  (20): (5510-5530).  [PMID:30309671]
21. Heng H, Wang Z, Li H, Huang Y, Lan Q, Guo X, Zhang L, Zhi Y, Cai J, Qin T, Xiang L, Wang S, Chen Y, Lu T, Lu S..  (2019)  Combining structure- and property-based optimization to identify selective FLT3-ITD inhibitors with good antitumor efficacy in AML cell inoculated mouse xenograft model..  Eur J Med Chem,  176  (248-267).  [PMID:31103903]
22. Yuan T, Qi B, Jiang Z, Dong W, Zhong L, Bai L, Tong R, Yu J, Shi J..  (2019)  Dual FLT3 inhibitors: Against the drug resistance of acute myeloid leukemia in recent decade..  Eur J Med Chem,  178  (468-483).  [PMID:31207462]
23. Mathison CJN, Chianelli D, Rucker PV, Nelson J, Roland J, Huang Z, Yang Y, Jiang J, Xie YF, Epple R, Bursulaya B, Lee C, Gao MY, Shaffer J, Briones S, Sarkisova Y, Galkin A, Li L, Li N, Li C, Hua S, Kasibhatla S, Kinyamu-Akunda J, Kikkawa R, Molteni V, Tellew JE..  (2020)  Efficacy and Tolerability of Pyrazolo[1,5-a]pyrimidine RET Kinase Inhibitors for the Treatment of Lung Adenocarcinoma..  ACS Med Chem Lett,  11  (4): (558-565).  [PMID:32292564]
24. Nair S, Kumar SR, Paidi VR, Sistla R, Kantheti D, Polimera SR, Thangavel S, Mukherjee AJ, Das M, Bhide RS, Pitts WJ, Murugesan N, Dudhgoankar S, Nagar J, Subramani S, Mazumder D, Carman JA, Holloway DA, Li X, Fereshteh MP, Ruepp S, Palanisamy K, Mariappan TT, Maddi S, Saxena A, Elzinga P, Chimalakonda A, Ruan Q, Ghosh K, Bose S, Sack J, Yan C, Kiefer SE, Xie D, Newitt JA, Saravanakumar SP, Rampulla RA, Barrish JC, Carter PH, Hynes J..  (2020)  Optimization of Nicotinamides as Potent and Selective IRAK4 Inhibitors with Efficacy in a Murine Model of Psoriasis..  ACS Med Chem Lett,  11  (7): (1402-1409).  [PMID:32676146]
25. Mark, M R MR and 5 more authors..  (1994)  rse, a novel receptor-type tyrosine kinase with homology to Axl/Ufo, is expressed at high levels in the brain..  The Journal of biological chemistry,    (8):   [PMID:7511603]
26. Toshima, J J, Ohashi, K K, Iwashita, S S and Mizuno, K K..  (1995)  Autophosphorylation activity and association with Src family kinase of Sky receptor tyrosine kinase..  Biochemical and biophysical research communications,    (17):   [PMID:7537495]
27. Godowski, P J PJ and 5 more authors..  (1995)  Reevaluation of the roles of protein S and Gas6 as ligands for the receptor tyrosine kinase Rse/Tyro 3..  Cell,    (11):   [PMID:7634325]
28. Crosier, K E KE and 6 more authors..  (1994)  Isolation and characterization of the human DTK receptor tyrosine kinase..  Growth factors (Chur, Switzerland),      [PMID:7857658]
29. Ohashi, K K, Mizuno, K K, Kuma, K K, Miyata, T T and Nakamura, T T..  (1994)  Cloning of the cDNA for a novel receptor tyrosine kinase, Sky, predominantly expressed in brain..  Oncogene,      [PMID:8108112]
30. Polvi, A A and 8 more authors..  (1993)  The human TYRO3 gene and pseudogene are located in chromosome 15q14-q25..  Gene,    (8):   [PMID:8262388]
31. Kajii, Y Y and 8 more authors..  (1996)  A tyrosine kinase-like molecule is localized in the nuclear membrane of neurons: hippocampal behavior under stress..  Biology of the cell,      [PMID:9175267]
32. Hafizi, Sassan S and Dahlbäck, Björn B..  (2006)  Signalling and functional diversity within the Axl subfamily of receptor tyrosine kinases..  Cytokine & growth factor reviews,      [PMID:16737840]
33. Shimojima, Masayuki M and 8 more authors..  (2006)  Tyro3 family-mediated cell entry of Ebola and Marburg viruses..  Journal of virology,      [PMID:17005688]
34. Cosemans, J M E M JM and 10 more authors..  (2010)  Potentiating role of Gas6 and Tyro3, Axl and Mer (TAM) receptors in human and murine platelet activation and thrombus stabilization..  Journal of thrombosis and haemostasis : JTH,      [PMID:20546121]
35. Shimojima, Masayuki M, Ströher, Ute U, Ebihara, Hideki H, Feldmann, Heinz H and Kawaoka, Yoshihiro Y..  (2012)  Identification of cell surface molecules involved in dystroglycan-independent Lassa virus cell entry..  Journal of virology,      [PMID:22156524]
36. Shimojima, Masayuki and Kawaoka, Yoshihiro..  (2012)  Cell surface molecules involved in infection mediated by lymphocytic choriomeningitis virus glycoprotein..  The Journal of veterinary medical science,      [PMID:22673088]

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