Recombinant Human YKL-40/CHI3L1 Protein

Features and benefits
  • Expression System: HEK293
  • Protein Tag: C-His
  • Bioactivity: Testing in progress
  • Endotoxin Concentration: <1.0 EU/μg
Item Number
rp169594
Grouped product items
SKUSizeAvailabilityPrice Qty
rp169594-10μg (Trial Size)
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10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$139.90
rp169594-50μg
50μg
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Production requires sourcing of materials. We appreciate your patience and understanding.
$399.90
rp169594-100μg
100μg
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$599.90
rp169594-1mg
1mg
Available within 8-12 weeks(?)
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$2,999.90

Animal Free, ≥95% (SDS-PAGE), 293F cell, C-His tag, 1-383 aa

Basic Description

Product NameRecombinant Human YKL-40/CHI3L1 Protein
GradeAnimal Free, Carrier Free
Product Description

Protein Function:
Chitinase 3-like 1 (CHI3L1), also called YKL-40 or human cartilage glycoprotein 39 (HCGP39), is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family. CHI3L1 was first identified as secreted from cultured articular chondrocytes, synovial cells, and activated monocyte-derived macrophages, but it is also secreted by neutrophils, endothelial cells, vascular smooth muscle cells, and some cancer cells. The human CHI3L1 cDNA encodes 383 amino acids (aa), including a 21 aa signal sequence and a 362 aa mature region with two intramolecular disulfides. Mature human CHI3L1 shares 73%, 80%, 82%, 83%, 86% and 87% aa sequence identity with mouse, rat, canine, bovine, porcine and equine CHI3L1, respectively. Human CHI3L1 and CHI3L2 share 43% aa sequence identity. Neither shows chitotriosidase activity, but both bind chitin and are thus termed chi-lectins. CHI3L1 can bind heparins, probably as heparan sulfate. It has been found to enhance cell adhesion and promote cell signaling, proliferation and tumor angiogenesis. Elevated serum CHI3L1 levels occur in some conditions characterized by inflammation and connective tissue remodeling, such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis. Single nucleotide polymorphisms that can increase serum CHI3L1 are associated with higher risk for asthma in childhood. CHI3L1 is frequently upregulated in glioblastoma, myxoid chondrosarcoma, melanoma and carcinomas of the breast, thyroid, colon, lung, kidney, and ovary. In asthma and cancer, serum CHI3L1 concentrations can correlate with prognosis.

Specifications & PurityAnimal Free, Carrier Free, ≥95%(SDS-PAGE)
BioactivityTesting in progress
Endotoxin Concentration<1.0 EU/μg
Expression SystemHEK293
Amino Acids1-383 aa
SequenceMGVKASQTGFVVLVLLQCCSAYKLVCYYTSWSQYREGDGSCFPDALDRFLCTHIIYSFANISNDHIDTWEWNDVTLYGMLNTLKNRNPNLKTLLSVGGWNFGSQRFSKIASNTQSRRTFIKSVPPFLRTHGFDGLDLAWLYPGRRDKQHFTTLIKEMKAEFIKEAQPGKKQLLLSAALSAGKVTIDSSYDIAKISQHLDFISIMTYDFHGAWRGTTGHHSPLFRGQEDASPDRFSNTDYAVGYMLRLGAPASKLV
Protein TagC-His
Predicted molecular weight43.6 kDa
SDS-PAGE42.3 kDa, under reducing condition; 42.1 & 79.5 kDa, under non-reducing condition

Images

Recombinant Human YKL-40/CHI3L1 Protein (rp169594) - SDS-PAGE
3 μg/lane of Recombinant Human YKL-40/CHI3L1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 42.3 kDa under reducing conditions and 42.1 & 79.5 kDa under non-reducing conditions.

Product Specifications

ReconstitutionWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilu
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at -20°C for 1 year. Avoid freeze / thaw cycle.

Certificates

Certificate of Analysis(COA)

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4 results found

Lot NumberCertificate TypeDateItem
ZJ24F0707639Certificate of AnalysisJul 03, 2024 rp169594
ZJ24F0707638Certificate of AnalysisJul 03, 2024 rp169594
ZJ24F0707637Certificate of AnalysisJul 03, 2024 rp169594
ZJ24R0600639Certificate of AnalysisJun 11, 2024 rp169594

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