Recombinant; Rabbit anti Human HuR/ELAVL1 Antibody; WB; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab109133-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $59.90 | |
| Ab109133-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $179.90 | |
| Ab109133-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $279.90 | |
| Ab109133-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,599.90 |
Recombinant; Rabbit anti Human HuR/ELAVL1 Antibody; WB; Unconjugated
| Product Name | Recombinant HuR/ELAVL1 Antibody - Primary antibody, specific to HuR/ELAVL1, Rabbit IgG |
|---|---|
| Synonyms | Hu-antigen R | HuR | ELAV (embryonic lethal abnormal vision Drosophila) like 1 antibody | ELAV (embryonic lethal, abnormal vision, Drosophila) like 1 (Hu antigen R) antibody | ELAV like 1 antibody | ELAV like RNA binding protein 1 antibody | ELAV-like pro |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.8 mg/mL |
| Host species | Rabbit |
| Specificity | HuR/ELAVL1 |
| Immunogen | A synthetic peptide derived from human HuR / ELAVL1 (AA 52-96) |
| Positive Control | WB: Mouse heart, LNCaP, A549, Hep G2, MCF7, HeLa and HEK293 lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human HuR/ELAVL1 Antibody, Recombinant, could be used for WB and so on. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.8 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant HuR/ELAVL1 Antibody (Ab109133) - Western Blot
All lanes: Recombinant HuR/ELAVL1 Antibody (Ab109133) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 33 kDa
Exposure time: 4.5 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0708761 | Certificate of Analysis | Jul 25, 2024 | Ab109133 |
| ZJ24F0708760 | Certificate of Analysis | Jul 25, 2024 | Ab109133 |
| 1. Meisner NC, Hintersteiner M, Mueller K, Bauer R, Seifert JM, Naegeli HU, Ottl J, Oberer L, Guenat C, Moss S, Harrer N, Woisetschlaeger M, Buehler C, Uhl V, Auer M.. (2007) Identification and mechanistic characterization of low-molecular-weight inhibitors for HuR.. Nat Chem Biol, 3 (8): (508-515). [PMID:17632515] |
| 2. Volpe SD, Listro R, Parafioriti M, Di Giacomo M, Rossi D, Ambrosio FA, Costa G, Alcaro S, Ortuso F, Hirsch AKH, Vasile F, Collina S.. (2020) BOPC1 Enantiomers Preparation and HuR Interaction Study. From Molecular Modeling to a Curious DEEP-STD NMR Application.. ACS Med Chem Lett, 11 (5): (883-888). [PMID:32435400] |
| 3. Ma, W J WJ, Cheng, S S, Campbell, C C, Wright, A A and Furneaux, H H.. (1996) Cloning and characterization of HuR, a ubiquitously expressed Elav-like protein.. The Journal of biological chemistry, (5): [PMID:8626503] |
| 4. Gallouzi, I E IE and Steitz, J A JA.. (2001) Delineation of mRNA export pathways by the use of cell-permeable peptides.. Science (New York, N.Y.), (30): [PMID:11729309] |
| 5. Li, Hongwei H and 7 more authors.. (2002) Lipopolysaccharide-induced methylation of HuR, an mRNA-stabilizing protein, by CARM1. Coactivator-associated arginine methyltransferase.. The Journal of biological chemistry, (22): [PMID:12237300] |
| 6. Tran, Hoanh H, Maurer, Fabienne F and Nagamine, Yoshikuni Y.. (2003) Stabilization of urokinase and urokinase receptor mRNAs by HuR is linked to its cytoplasmic accumulation induced by activated mitogen-activated protein kinase-activated protein kinase 2.. Molecular and cellular biology, [PMID:14517288] |
| 7. Andersen, Jens S JS and 5 more authors.. (2003) Proteomic characterization of the human centrosome by protein correlation profiling.. Nature, (4): [PMID:14654843] |
| 8. Tran, Hoanh H, Schilling, Marcel M, Wirbelauer, Christiane C, Hess, Daniel D and Nagamine, Yoshikuni Y.. (2004) Facilitation of mRNA deadenylation and decay by the exosome-bound, DExH protein RHAU.. Molecular cell, (16): [PMID:14731398] |
| 9. Grimwood, Jane J and 97 more authors.. (2004) The DNA sequence and biology of human chromosome 19.. Nature, (1): [PMID:15057824] |
| 10. Yu, Li-Rong LR and 5 more authors.. (2007) Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra.. Journal of proteome research, [PMID:17924679] |
| 11. Höck, Julia J and 7 more authors.. (2007) Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells.. EMBO reports, [PMID:17932509] |
| 12. Sano, Etsuko E and 5 more authors.. (2008) Novel tyrosine phosphorylated and cardiolipin-binding protein CLPABP functions as mitochondrial RNA granule.. Biochimica et biophysica acta, [PMID:18191643] |
| 13. Doller, Anke A and 6 more authors.. (2008) Posttranslational modification of the AU-rich element binding protein HuR by protein kinase Cdelta elicits angiotensin II-induced stabilization and nuclear export of cyclooxygenase 2 mRNA.. Molecular and cellular biology, [PMID:18285462] |
| 14. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648] |
| 15. Weidensdorfer, Doreen D and 8 more authors.. (2009) Control of c-myc mRNA stability by IGF2BP1-associated cytoplasmic RNPs.. RNA (New York, N.Y.), [PMID:19029303] |
| 16. Ray, Debashish D and 8 more authors.. (2009) Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.. Nature biotechnology, [PMID:19561594] |
| 17. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 18. Benoit, Roger Marc RM and 8 more authors.. (2010) The x-ray crystal structure of the first RNA recognition motif and site-directed mutagenesis suggest a possible HuR redox sensing mechanism.. Journal of molecular biology, (16): [PMID:20219472] |
| 19. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 20. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 21. Wang, Hong H and 7 more authors.. (2013) The structure of the ARE-binding domains of Hu antigen R (HuR) undergoes conformational changes during RNA binding.. Acta crystallographica. Section D, Biological crystallography, [PMID:23519412] |
| 22. Wächter, Kristin K, Köhn, Marcel M, Stöhr, Nadine N and Hüttelmaier, Stefan S.. (2013) Subcellular localization and RNP formation of IGF2BPs (IGF2 mRNA-binding proteins) is modulated by distinct RNA-binding domains.. Biological chemistry, [PMID:23640942] |
| 23. Guo, Ailan and 15 more authors.. (2014) Immunoaffinity enrichment and mass spectrometry analysis of protein methylation.. Molecular & cellular proteomics : MCP, [PMID:24129315] |
| 24. Nishino, Tasuku T and 11 more authors.. (2016) Antagonizing effect of CLPABP on the function of HuR as a regulator of ARE-containing leptin mRNA stability and the effect of its depletion on obesity in old male mouse.. Biochimica et biophysica acta, [PMID:27616329] |
| 25. Balinsky, Corey A CA and 6 more authors.. (2017) IRAV (FLJ11286), an Interferon-Stimulated Gene with Antiviral Activity against Dengue Virus, Interacts with MOV10.. Journal of virology, (1): [PMID:27974568] |
| 26. Maeda, Akane A, Uchida, Moe M, Nishikawa, Sumire S, Nishino, Tasuku T and Konishi, Hiroaki H.. (2018) Role of N-myristoylation in stability and subcellular localization of the CLPABP protein.. Biochemical and biophysical research communications, (1): [PMID:29180010] |
| 27. Huang, Huilin and 29 more authors.. (2018) Recognition of RNA N6-methyladenosine by IGF2BP proteins enhances mRNA stability and translation.. Nature cell biology, [PMID:29476152] |
| 28. Chen, Xin X and 22 more authors.. (2019) 5-methylcytosine promotes pathogenesis of bladder cancer through stabilizing mRNAs.. Nature cell biology, [PMID:31358969] |
| 29. Zhu, Song and 9 more authors.. (2020) An oncopeptide regulates m6A recognition by the m6A reader IGF2BP1 and tumorigenesis.. Nature communications, (3): [PMID:32245947] |
