Recombinant; Rabbit anti Human IFITM1 Antibody; WB, IHC, IF, ICC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab109412-10μl | 10μl | In stock | $69.90 | |
| Ab109412-50μl | 50μl | In stock | $199.90 | |
| Ab109412-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $309.90 | |
| Ab109412-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human IFITM1 Antibody; WB, IHC, IF, ICC; Unconjugated
| Product Name | Recombinant IFITM1 Antibody, specific to IFITM1, Rabbit IgG |
|---|---|
| Synonyms | Dispanin subfamily A member 2a | DSPA2a | Interferon-induced protein 17 | Interferon-inducible protein 9-27 | Leu-13 antigen | CD225 antigen | CD225 | CD225Leu-13 antigen | IFI17 | IFI17LEU13,9-27 | IFITM1 | interferon induced transmembrane protein 1 (9-2 |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.6 mg/mL |
| Host species | Rabbit |
| Specificity | IFITM1 |
| Immunogen | A synthetic peptide derived from human IFITM1 (AA 13-30) |
| Positive Control | WB: K562 and HeLa cell lysates. IHC:Human breast cancer tissue. IF/ICC: K562 cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human IFITM1 Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 IF/ICC: 1/50-1/200 Protein Function IFN-induced antiviral protein that mediate cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus, and dengue virus by inhibiting the early step(s) of replication. Plays a key role in the antiproliferative action of IFN-gamma either by inhibiting the ERK activition or by arresting cell growth in G1 phase in a p53-dependent manner. Implicated in the control of cell growth. Component of a multimeric complex involved in the transduction of antiproliferative and homotypic adhesion signals. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.6 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant IFITM1 Antibody (Ab109412) - Western Blot
All lanes: Recombinant IFITM1 Antibody (Ab109412) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 16 kDa
Exposure time: 3.7 s
Recombinant IFITM1 Antibody (Ab109412) - IF/ICC
Immunocytochemistry analysis of IFITM1 (green) in K562 using Recombinant IFITM1 Antibody (Ab109412), and cells were counterstained with DAPI (blue).
Recombinant IFITM1 Antibody (Ab109412) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Recombinant IFITM1 Antibody (Ab109412). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50 - 1/100 |
| IF/ICC | 1/50 - 1/200 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0506063 | Certificate of Analysis | May 24, 2024 | Ab109412 |
| ZJ24F0506064 | Certificate of Analysis | May 24, 2024 | Ab109412 |
| 1. Todd D Taylor,Hideki Noguchi,Yasushi Totoki,Atsushi Toyoda,Yoko Kuroki,Ken Dewar,Christine Lloyd,Takehiko Itoh,Tadayuki Takeda,Dae-Won Kim,Xinwei She,Karen F Barlow,Toby Bloom,Elspeth Bruford,Jean L Chang,Christina A Cuomo,Evan Eichler,Michael G FitzGerald,David B Jaffe,Kurt LaButti,Robert Nicol,Hong-Seog Park,Christopher Seaman,Carrie Sougnez,Xiaoping Yang,Andrew R Zimmer,Michael C Zody,Bruce W Birren,Chad Nusbaum,Asao Fujiyama,Masahira Hattori,Jane Rogers,Eric S Lander,Yoshiyuki Sakaki. (2006-03-24) Human chromosome 11 DNA sequence and analysis including novel gene identification.. Nature, 440 ((7083)): (497-500). [PMID:16554811] |
| 2. Bradbury, L E LE, Kansas, G S GS, Levy, S S, Evans, R L RL and Tedder, T F TF.. (1992) The CD19/CD21 signal transducing complex of human B lymphocytes includes the target of antiproliferative antibody-1 and Leu-13 molecules.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:1383329] |
| 3. Takahashi, S S, Doss, C C, Levy, S S and Levy, R R.. (1990) TAPA-1, the target of an antiproliferative antibody, is associated on the cell surface with the Leu-13 antigen.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:2398277] |
| 4. Reid, L E LE and 6 more authors.. (1989) A single DNA response element can confer inducibility by both alpha- and gamma-interferons.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2492664] |
| 5. Deblandre, G A GA and 7 more authors.. (1995) Expression cloning of an interferon-inducible 17-kDa membrane protein implicated in the control of cell growth.. The Journal of biological chemistry, (6): [PMID:7559564] |
| 6. Xu, Ye Y, Yang, Guohua G and Hu, Gengxi G.. (2009) Binding of IFITM1 enhances the inhibiting effect of caveolin-1 on ERK activation.. Acta biochimica et biophysica Sinica, [PMID:19499152] |
| 7. Brass, Abraham L AL and 13 more authors.. (2009) The IFITM proteins mediate cellular resistance to influenza A H1N1 virus, West Nile virus, and dengue virus.. Cell, (24): [PMID:20064371] |
| 8. Yu, Fang F and 7 more authors.. (2011) Knockdown of interferon-induced transmembrane protein 1 (IFITM1) inhibits proliferation, migration, and invasion of glioma cells.. Journal of neuro-oncology, [PMID:20838853] |
| 9. Siegrist, Fredy F, Ebeling, Martin M and Certa, Ulrich U.. (2011) The small interferon-induced transmembrane genes and proteins.. Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, [PMID:21166591] |
| 10. Lu, Jennifer J and 5 more authors.. (2011) The IFITM proteins inhibit HIV-1 infection.. Journal of virology, [PMID:21177806] |
| 11. Huang, I-Chueh IC and 17 more authors.. (2011) Distinct patterns of IFITM-mediated restriction of filoviruses, SARS coronavirus, and influenza A virus.. PLoS pathogens, (6): [PMID:21253575] |
| 12. Raychoudhuri, Amit A and 5 more authors.. (2011) ISG56 and IFITM1 proteins inhibit hepatitis C virus replication.. Journal of virology, [PMID:21976647] |
| 13. Sällman Almén, Markus M, Bringeland, Nathalie N, Fredriksson, Robert R and Schiöth, Helgi B HB.. (2012) The dispanins: a novel gene family of ancient origin that contains 14 human members.. PloS one, [PMID:22363774] |
| 14. Chan, Ying Kai YK, Huang, I-Chueh IC and Farzan, Michael M.. (2012) IFITM proteins restrict antibody-dependent enhancement of dengue virus infection.. PloS one, [PMID:22479637] |
| 15. Kim, Beom-Su BS and 11 more authors.. (2012) IFITM1 increases osteogenesis through Runx2 in human alveolar-derived bone marrow stromal cells.. Bone, [PMID:22634173] |
| 16. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 17. Weston, Stuart and 5 more authors.. (2014) A membrane topology model for human interferon inducible transmembrane protein 1.. PloS one, [PMID:25105503] |
| 18. Narayana, Sumudu K SK and 7 more authors.. (2015) The Interferon-induced Transmembrane Proteins, IFITM1, IFITM2, and IFITM3 Inhibit Hepatitis C Virus Entry.. The Journal of biological chemistry, (23): [PMID:26354436] |
| 19. Shi, Guoli and 10 more authors.. (2021) Opposing activities of IFITM proteins in SARS-CoV-2 infection.. The EMBO journal, (1): [PMID:33270927] |
