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Recombinant Integrin beta 3 Antibody - Primary antibody, specific to ITGB3, Rabbit IgG

  • ExactAb™
  • Recombinant
  • Validated
  • 0.6 mg/mL
Features and benefits
  • Short Overview:

    Recombinant; Rabbit anti Human Integrin beta 3 Antibody; WB; Unconjugated

  • Species reactivity(Reacts with): Human,Mouse,Rat
  • Isotype: Rabbit IgG
    Application:
  • WB
Item Number
Ab110875
Grouped product items
SKUSizeAvailabilityPrice Qty
Ab110875-10μl
10μl
In stock
$86.90
Ab110875-50μl
50μl
In stock
$249.90
Ab110875-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$389.90
Ab110875-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,899.90

Recombinant; Rabbit anti Human Integrin beta 3 Antibody; WB; Unconjugated

Basic Description

Product NameRecombinant Integrin beta 3 Antibody - Primary antibody, specific to ITGB3, Rabbit IgG
SynonymsITGB3; GT; GT2; CD61; GP3A; BDPLT2; GPIIIa; BDPLT16; BDPLT24; BDPLT16 antibody; BDPLT2 antibody; CD 61 antibody; CD61 antibody; CD61 antigen antibody; GP3A antibody; GPIIIa antibody; GT antibody; HPA 1 antibody; HPA 4 antibody; Integrin beta 3 (platelet g
Specifications & PurityExactAb™, Validated, Recombinant, 0.6 mg/mL
Host speciesRabbit
SpecificityITGB3
ImmunogenRecombinant protein of human Integrin beta 3 (AA 27-377).
Positive ControlWB: HeLa, NIH/3T3 and C6 cell lysates.
ConjugationUnconjugated
GradeExactAb™, Recombinant, Validated
Product Description

Rabbit anti Human Integrin beta 3 Antibody, Recombinant, could be used for WB and so on.
Application:
WB: 1/500-1/1000
Protein Function
Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

Product Properties

IsotypeRabbit IgG
SDS-PAGE150 kDa
Purification MethodAntigen affinity purified
FormLiquid
Concentration0.6 mg/mL
Storage TempStore at -20°C,Avoid repeated freezing and thawing
Shipped InIce chest + Ice pads
Stability And StorageStore at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant Integrin beta 3 Antibody (Ab110875) - Western Blot
All lanes: Recombinant Integrin beta 3 Antibody (Ab110875) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/50000 dilution

Predicted band size: 87 kDa
Observed band size: 99 kDa
Exposure time: 52.4 s

Associated Targets

ITGB3 Tclin Integrin beta-3 0 Activities

Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)

Application

ApplicationDilution info
WB

1/500-1/1000

Certificates

Certificate of Analysis(COA)

Enter Lot Number to search for COA:

To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot NumberCertificate TypeDateItem
ZJ24F0202730Certificate of AnalysisFeb 05, 2024 Ab110875
ZJ24F0202729Certificate of AnalysisFeb 05, 2024 Ab110875

Related Documents

References

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61. Nurden, Alan T AT and 6 more authors..  (2002)  A novel 196Leu to Pro substitution in the beta3 subunit of the alphaIIbbeta3 integrin in a patient with a variant form of Glanzmann thrombasthenia..  Platelets,      [PMID:11897046]
62. Obergfell, Achim A and 7 more authors..  (2002)  Coordinate interactions of Csk, Src, and Syk kinases with [alpha]IIb[beta]3 initiate integrin signaling to the cytoskeleton..  The Journal of cell biology,    (15):   [PMID:11940607]
63. Jallu, Vincent V, Meunier, Marc M, Brément, Maryline M and Kaplan, Cécile C..  (2002)  A new platelet polymorphism Duv(a+), localized within the RGD binding domain of glycoprotein IIIa, is associated with neonatal thrombocytopenia..  Blood,    (15):   [PMID:12036875]
64. D'Andrea, Giovanna G and 6 more authors..  (2002)  Glanzmann's thrombasthenia: identification of 19 new mutations in 30 patients..  Thrombosis and haemostasis,      [PMID:12083483]
65. Weber, Artur-Aron AA and 8 more authors..  (2002)  Low incidence of paradoxical platelet activation by glycoprotein IIb/IIIa inhibitors..  Thrombosis research,    (1):   [PMID:12165285]
66. Nair, Sona S and 5 more authors..  (2002)  Two new beta3 integrin mutations in Indian patients with Glanzmann thrombasthenia: localization of mutations affecting cysteine residues in integrin beta3..  Thrombosis and haemostasis,      [PMID:12353082]
67. Gevaert, Kris K and 6 more authors..  (2003)  Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides..  Nature biotechnology,      [PMID:12665801]
68. Lin, Cristiane G CG and 6 more authors..  (2003)  CCN3 (NOV) is a novel angiogenic regulator of the CCN protein family..  The Journal of biological chemistry,    (27):   [PMID:12695522]
69. Ibbotson, Tim T, McGavin, Jane K JK and Goa, Karen L KL..  (2003)  Abciximab: an updated review of its therapeutic use in patients with ischaemic heart disease undergoing percutaneous coronary revascularisation..  Drugs,      [PMID:12749745]
70. Bax, Daniel V DV and 7 more authors..  (2003)  Cell adhesion to fibrillin-1 molecules and microfibrils is mediated by alpha 5 beta 1 and alpha v beta 3 integrins..  The Journal of biological chemistry,    (5):   [PMID:12807887]
71. Auer, Johann J and 5 more authors..  (2003)  [Platelet glycoprotein IIb/IIIa inhibitors in coronary artery disease]..  Herz,      [PMID:12928738]
72. Schrör, Karsten K and Weber, Artur-Aron AA..  (2003)  Comparative pharmacology of GP IIb/IIIa antagonists..  Journal of thrombosis and thrombolysis,      [PMID:14618072]
73. Zhang, Hongquan H and 8 more authors..  (2004)  Myosin-X provides a motor-based link between integrins and the cytoskeleton..  Nature cell biology,      [PMID:15156152]
74. Jordan, Peter A PA and 6 more authors..  (2005)  A role for the thiol isomerase protein ERP5 in platelet function..  Blood,    (15):   [PMID:15466936]
75. González-Manchón, Consuelo C and 6 more authors..  (2004)  A variant thrombasthenic phenotype associated with compound heterozygosity of integrin beta3-subunit: (Met124Val)beta3 alters the subunit dimerization rendering a decreased number of constitutive active alphaIIbbeta3 receptors..  Thrombosis and haemostasis,      [PMID:15583747]
76. Tanaka, S S and 6 more authors..  (2005)  Double heterozygosity for a novel missense mutation of Ile304 to Asn in addition to the missense mutation His280 to Pro in the integrin beta3 gene as a cause of the absence of platelet alphaIIbbeta3 in Glanzmann's thrombasthenia..  Journal of thrombosis and haemostasis : JTH,      [PMID:15634267]
77. Nair, S S, Ghosh, K K, Shetty, S S and Mohanty, D D..  (2005)  Mutations in GPIIIa molecule as a cause for Glanzmann thrombasthenia in Indian patients..  Journal of thrombosis and haemostasis : JTH,      [PMID:15748237]
78. Wang, Xin X, Huang, David Y DY, Huong, Shu-Mei SM and Huang, Eng-Shang ES..  (2005)  Integrin alphavbeta3 is a coreceptor for human cytomegalovirus..  Nature medicine,      [PMID:15834425]
79. Chen, Faye Hui FH, Thomas, Ashby O AO, Hecht, Jacqueline T JT, Goldring, Mary B MB and Lawler, Jack J..  (2005)  Cartilage oligomeric matrix protein/thrombospondin 5 supports chondrocyte attachment through interaction with integrins..  The Journal of biological chemistry,    (23):   [PMID:16051604]
80. Wadehra, Madhuri M and 6 more authors..  (2005)  Epithelial membrane protein-2 regulates surface expression of alphavbeta3 integrin in the endometrium..  Developmental biology,    (15):   [PMID:16216233]
81. Lewandrowski, Urs U, Moebius, Jan J, Walter, Ulrich U and Sickmann, Albert A..  (2006)  Elucidation of N-glycosylation sites on human platelet proteins: a glycoproteomic approach..  Molecular & cellular proteomics : MCP,      [PMID:16263699]
82. and Bennett, Joel S JS..  (2005)  Structure and function of the platelet integrin alphaIIbbeta3..  The Journal of clinical investigation,      [PMID:16322781]
83. Liu, Tao T and 6 more authors..  ()  Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry..  Journal of proteome research,      [PMID:16335952]
84. Hall, Pamela R PR and 5 more authors..  (2007)  Characterization and NMR solution structure of a novel cyclic pentapeptide inhibitor of pathogenic hantaviruses..  Chemical biology & drug design,      [PMID:17441904]
85. Parry, Christian S CS, Gorski, Jack J and Stern, Lawrence J LJ..  (2007)  Crystallographic structure of the human leukocyte antigen DRA, DRB3*0101: models of a directional alloimmune response and autoimmunity..  Journal of molecular biology,    (10):   [PMID:17583734]
86. Ma, Y-Q YQ, Qin, J J and Plow, E F EF..  (2007)  Platelet integrin alpha(IIb)beta(3): activation mechanisms..  Journal of thrombosis and haemostasis : JTH,      [PMID:17635696]
87. Garrigues, H Jacques HJ, Rubinchikova, Yelena E YE, Dipersio, C Michael CM and Rose, Timothy M TM..  (2008)  Integrin alphaVbeta3 Binds to the RGD motif of glycoprotein B of Kaposi's sarcoma-associated herpesvirus and functions as an RGD-dependent entry receptor..  Journal of virology,      [PMID:18045938]
88. Ghevaert, Cedric C and 14 more authors..  (2008)  A nonsynonymous SNP in the ITGB3 gene disrupts the conserved membrane-proximal cytoplasmic salt bridge in the alphaIIbbeta3 integrin and cosegregates dominantly with abnormal proplatelet formation and macrothrombocytopenia..  Blood,    (1):   [PMID:18065693]
89. Zahedi, René P RP and 8 more authors..  (2008)  Phosphoproteome of resting human platelets..  Journal of proteome research,      [PMID:18088087]
90. Mori, Seiji S and 10 more authors..  (2008)  Direct binding of integrin alphavbeta3 to FGF1 plays a role in FGF1 signaling..  The Journal of biological chemistry,    (27):   [PMID:18441324]
91. Mazzaferri, Ernest L EL and Young, John J JJ..  (2008)  Abciximab: a review and update for clinicians..  Expert review of cardiovascular therapy,      [PMID:18510478]
92. Saegusa, Jun J and 8 more authors..  (2008)  Pro-inflammatory secretory phospholipase A2 type IIA binds to integrins alphavbeta3 and alpha4beta1 and induces proliferation of monocytic cells in an integrin-dependent manner..  The Journal of biological chemistry,    (19):   [PMID:18635536]
93. Mikelis, Constantinos C, Sfaelou, Evanthia E, Koutsioumpa, Marina M, Kieffer, Nelly N and Papadimitriou, Evangelia E..  (2009)  Integrin alpha(v)beta(3) is a pleiotrophin receptor required for pleiotrophin-induced endothelial cell migration through receptor protein tyrosine phosphatase beta/zeta..  FASEB journal : official publication of the Federation of American Societies for Experimental Biology,      [PMID:19141530]
94. Jallu, Vincent V and 7 more authors..  (2010)  AlphaIIbbeta3 integrin: new allelic variants in Glanzmann thrombasthenia, effects on ITGA2B and ITGB3 mRNA splicing, expression, and structure-function..  Human mutation,      [PMID:20020534]
95. Ieguchi, Katsuaki K and 8 more authors..  (2010)  Direct binding of the EGF-like domain of neuregulin-1 to integrins ({alpha}v{beta}3 and {alpha}6{beta}4) is involved in neuregulin-1/ErbB signaling..  The Journal of biological chemistry,    (8):   [PMID:20682778]
96. Bledzka, Kamila K and 7 more authors..  (2010)  Tyrosine phosphorylation of integrin beta3 regulates kindlin-2 binding and integrin activation..  The Journal of biological chemistry,    (1):   [PMID:20702409]
97. Fujita, Masaaki M, Takada, Yoko K YK and Takada, Yoshikazu Y..  (2012)  Integrins αvβ3 and α4β1 act as coreceptors for fractalkine, and the integrin-binding defective mutant of fractalkine is an antagonist of CX3CR1..  Journal of immunology (Baltimore, Md. : 1950),    (15):   [PMID:23125415]
98. Schmidt, Katja and 6 more authors..  (2013)  Integrins modulate the infection efficiency of West Nile virus into cells..  The Journal of general virology,      [PMID:23658209]
99. Wei, Yongwei Y and 7 more authors..  (2014)  Roles of the putative integrin-binding motif of the human metapneumovirus fusion (f) protein in cell-cell fusion, viral infectivity, and pathogenesis..  Journal of virology,      [PMID:24478423]
100. Fujita, Masaaki M, Takada, Yoko K YK and Takada, Yoshikazu Y..  (2014)  The chemokine fractalkine can activate integrins without CX3CR1 through direct binding to a ligand-binding site distinct from the classical RGD-binding site..  PloS one,      [PMID:24789099]
101. Fujita, Masaaki M and 7 more authors..  (2015)  Proinflammatory secreted phospholipase A2 type IIA (sPLA-IIA) induces integrin activation through direct binding to a newly identified binding site (site 2) in integrins αvβ3, α4β1, and α5β1..  The Journal of biological chemistry,    (2):   [PMID:25398877]
102. Hammes, Stephen R and Davis, Paul J..  (2015)  Overlapping nongenomic and genomic actions of thyroid hormone and steroids..  Best practice & research. Clinical endocrinology & metabolism,      [PMID:26303085]
103. Yu, Jessica J and 5 more authors..  (2017)  The CD9, CD81, and CD151 EC2 domains bind to the classical RGD-binding site of integrin αvβ3..  The Biochemical journal,    (15):   [PMID:27993971]
104. Mori, Seiji S and 10 more authors..  (2017)  The integrin-binding defective FGF2 mutants potently suppress FGF2 signalling and angiogenesis..  Bioscience reports,    (28):   [PMID:28302677]
105. Cedano Prieto, Dora Maria DM and 5 more authors..  (2017)  Direct integrin binding to insulin-like growth factor-2 through the C-domain is required for insulin-like growth factor receptor type 1 (IGF1R) signaling..  PloS one,      [PMID:28873464]
106. Takada, Yoko K YK and 5 more authors..  (2017)  Direct binding to integrins and loss of disulfide linkage in interleukin-1β (IL-1β) are involved in the agonistic action of IL-1β..  The Journal of biological chemistry,    (8):   [PMID:29030430]
107. Kazemi, Ahmad A and 6 more authors..  (2017)  Molecular characterization of Glanzmann's thrombasthenia in Iran: identification of three novel mutations..  Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis,      [PMID:29084015]
108. Fujita, Masaaki M, Davari, Parastoo P, Takada, Yoko K YK and Takada, Yoshikazu Y..  (2018)  Stromal cell-derived factor-1 (CXCL12) activates integrins by direct binding to an allosteric ligand-binding site (site 2) of integrins without CXCR4..  The Biochemical journal,    (16):   [PMID:29301984]
109. Miyashita, Naohiro N and 13 more authors..  (2018)  A novel heterozygous ITGB3 p.T720del inducing spontaneous activation of integrin αIIbβ3 in autosomal dominant macrothrombocytopenia with aggregation dysfunction..  Annals of hematology,      [PMID:29380037]
110. Takada, Yoko K; Yu, Jessica; Shimoda, Michiko and Takada, Yoshikazu..  (2019)  Integrin Binding to the Trimeric Interface of CD40L Plays a Critical Role in CD40/CD40L Signaling..  Journal of immunology (Baltimore, Md. : 1950),    (1):   [PMID:31331973]

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