Recombinant; Rabbit anti Human IQGAP1 Antibody; WB; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab111124-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $69.90 | |
| Ab111124-50μl | 50μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $199.90 | |
| Ab111124-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $299.90 | |
| Ab111124-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,699.90 |
Recombinant; Rabbit anti Human IQGAP1 Antibody; WB; Unconjugated
| Product Name | Recombinant IQGAP1 Antibody - Primary antibody, specific to IQGAP1, Rabbit IgG | |
|---|---|---|
| Synonyms | p195 | HUMORFA01 antibody | IQ motif containing GTPase activating protein 1 antibody | IQGA1_HUMAN antibody | Iqgap1 antibody | KIAA0051 antibody | p195 antibody | Ras GTPase activating like protein 1 antibody | Ras GTPase-activating-like protein IQGAP1 a | |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.35 mg/mL | |
| Host species | Rabbit | |
| Specificity | IQGAP1 | |
| Immunogen | A synthetic peptide derived from human IQGAP1 (AA 1-58) | |
| Positive Control | WB: A549, HeLa and HEK293 cell lysates. | |
| Conjugation | Unconjugated | |
| Grade | ExactAb™, Recombinant, Validated | |
| Product Description | Rabbit anti Human IQGAP1 Antibody, Recombinant, could be used for WB and so on. Application:
Protein Function Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth. Post-translational Phosphorylation of Ser-1443 by PKC prevents interaction between C1 and C2, allowing binding of nucleotide-free CDC42. Ser-1443 phosphorylation enhances the ability to promote neurite outgrowth. |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.35 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant IQGAP1 Antibody (Ab111124) - Western Blot
All lanes: Recombinant IQGAP1 Antibody (Ab111124) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 180 kDa
Observed band size: 211 kDa
Exposure time: 9.3 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0809196 | Certificate of Analysis | Aug 05, 2024 | Ab111124 |
| ZJ24F0809195 | Certificate of Analysis | Aug 05, 2024 | Ab111124 |
| 1. Tan L, Gurbani D, Weisberg EL, Jones DS, Rao S, Singer WD, Bernard FM, Mowafy S, Jenney A, Du G et al.. (2017) Studies of TAK1-centered polypharmacology with novel covalent TAK1 inhibitors.. Bioorg Med Chem, 25 (4): (1320-1328). [PMID:28038940] |
| 2. Nomura, N N and 9 more authors.. (1994) Prediction of the coding sequences of unidentified human genes. II. The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1.. DNA research : an international journal for rapid publication of reports on genes and genomes, [PMID:7584044] |
| 3. Weissbach, L L and 6 more authors.. (1994) Identification of a human rasGAP-related protein containing calmodulin-binding motifs.. The Journal of biological chemistry, (12): [PMID:8051149] |
| 4. Hart, M J MJ, Callow, M G MG, Souza, B B and Polakis, P P.. (1996) IQGAP1, a calmodulin-binding protein with a rasGAP-related domain, is a potential effector for cdc42Hs.. The EMBO journal, (17): [PMID:8670801] |
| 5. Grohmanova, Katarina K and 5 more authors.. (2004) Phosphorylation of IQGAP1 modulates its binding to Cdc42, revealing a new type of rho-GTPase regulator.. The Journal of biological chemistry, (19): [PMID:15355962] |
| 6. Li, Zhigang Z and 6 more authors.. (2005) IQGAP1 promotes neurite outgrowth in a phosphorylation-dependent manner.. The Journal of biological chemistry, (8): [PMID:15695813] |
| 7. Morita, Eiji E and 6 more authors.. (2007) Human ESCRT and ALIX proteins interact with proteins of the midbody and function in cytokinesis.. The EMBO journal, (3): [PMID:17853893] |
| 8. Kaur, Ramneet R, Yuan, Xin X, Lu, Michael L ML and Balk, Steven P SP.. (2008) Increased PAK6 expression in prostate cancer and identification of PAK6 associated proteins.. The Prostate, (1): [PMID:18642328] |
| 9. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648] |
| 10. Johnson, Michael M, Sharma, Manisha M, Brocardo, Mariana G MG and Henderson, Beric R BR.. (2011) IQGAP1 translocates to the nucleus in early S-phase and contributes to cell cycle progression after DNA replication arrest.. The international journal of biochemistry & cell biology, [PMID:20883816] |
| 11. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895] |
| 12. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378] |
| 13. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 14. Zhou, Ding'an D and 10 more authors.. (2013) SASH1 regulates melanocyte transepithelial migration through a novel Gαs-SASH1-IQGAP1-E-Cadherin dependent pathway.. Cellular signalling, [PMID:23333244] |
| 15. Lu, Jianhong J and 7 more authors.. (2013) Host IQGAP1 and Ebola virus VP40 interactions facilitate virus-like particle egress.. Journal of virology, [PMID:23637409] |
| 16. Liu, Jing J and 11 more authors.. (2017) The Primate-Specific Gene TMEM14B Marks Outer Radial Glia Cells and Promotes Cortical Expansion and Folding.. Cell stem cell, (2): [PMID:29033352] |
