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Recombinant Keap1 Antibody - Primary antibody, specific to KEAP1, Rabbit IgG

ExactAb™
Recombinant
Validated
0.9 mg/mL

Features and benefits

Short Overview:
Recombinant; Rabbit anti Human Keap1 Antibody; WB, IHC; Unconjugated
Species reactivity(Reacts with): Human,Mouse
Isotype: Rabbit IgG

Application:

Item Number

Ab111970

Grouped product items
SKU	Size	Availability	Price
Ab111970-10μl	10μl	In stock	$66.90
Ab111970-50μl	50μl	In stock	$189.90
Ab111970-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$279.90
Ab111970-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,399.90

Recombinant; Rabbit anti Human Keap1 Antibody; WB, IHC; Unconjugated

View related series

Accession#:Q14145 Gene ID:9817 KEAP1

Basic Description

Product Name	Recombinant Keap1 Antibody - Primary antibody, specific to KEAP1, Rabbit IgG
Synonyms	Cytosolic inhibitor of Nrf2 antibody; INrf 2 antibody; INrf2 antibody; Keap 1 antibody; KEAP1 antibody; KEAP1_HUMAN antibody; Kelch like ECH associated protein 1 antibody; Kelch like family member 19 antibody; Kelch like protein 19antibody; Kelch-like ECH
Specifications & Purity	ExactAb™, Validated, Recombinant, 0.9 mg/mL
Host species	Rabbit
Specificity	KEAP1
Immunogen	Recombinant protein of human Keap1 (AA 321-609)
Positive Control	WB: RAW 264.7, CTLL-2, HeLa, A549, MCF7 and Hep G2 cell lysates. IHC: Human breast cancer tissue.
Conjugation	Unconjugated
Grade	ExactAb™, Recombinant, Validated
Product Description	Rabbit anti Human Keap1 Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500 - 1/1000 IHC : 1/50 - 1/100 Protein Function Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome. Post-translational Ubiquitinated and subject to proteasomal degradation.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Antigen affinity purified
Form	Liquid
Concentration	0.9 mg/mL
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Recombinant Keap1 Antibody (Ab111970) - Western Blot
All lanes: Recombinant Keap1 Antibody (Ab111970) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 70 kDa
Observed band size: 64 kDa
Exposure time: 10.0 s

Recombinant Keap1 Antibody (Ab111970) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant Keap1 Antibody (Ab111970). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Associated Targets

KEAP1 Tclin Kelch-like ECH-associated protein 1 0 Activities

Discover Target: KEAP1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application

Application	Dilution info
WB	1/500-1/1000
IHC	1/50 - 1/100

Certificates

Certificate of Analysis(COA)

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To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section

2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0102502	Certificate of Analysis	Jan 16, 2024	Ab111970
ZJ24F0102501	Certificate of Analysis	Jan 16, 2024	Ab111970

FAQs and Articles

FAQ

Immunohistochemical staining protocol for frozen samples (IHC-Fr)

Categories: Protocols

Tags:

IHC Monoclonal antibodies Immunohistochemistry Frozen section Recombinant Antibodies

Immunohistochemistry (IHC) staining antigen retrieval protocol

Categories: Protocols

Tags:

IHC Monoclonal antibodies Immunohistochemistry Recombinant Antibodies Antigen Retrieval

References

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