Recombinant Kex2 Protease - ≥10 units/mg, high purity

Item Number

R141093

• Specifications & Purity: ≥10 units/mg
• Storage Temp: Store at -20°C
• Shipped In: Ice chest + Ice pads
• Grade: EnzymoPure™

Product Description

Kex2 is a Ca2+-dependent serine protease and cleaves at C-terminal site of Lys-Arg, Arg-Arg, Pro-Arg in pro-α,factor and killer-toxin precursors maturing, it was discovered in Saccharomyces cerevisiae.But Kex2 can’t recognize and cut a single basic amino acid,such as carboxyl end peptide bond of arginine and lysine. Recombinant Kex2 is a genetically engineered protein expressed in Pichia pastoris and purified by high pressure liquid chromatography. The optimal pH of Kex2 protease is 9.0, and the optimal temperature is 37 ℃.It is stable in buffer (pH 5.0-6.0).The activity of Kex2 is not affected by the conventional serine protease inhibitors such as PMSF, TPCK, TLCK inhibition. UNIT DEFINITION:One USP unit of Kex2 activity will catalyze 1μmol Boc-QRR-pNA per minute in a reaction volume of 3.0ml at pH8.0 and 25℃.