Recombinant; Rabbit anti Human Lamin B1 Antibody; WB, IHC; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab112659-10μl | 10μl | In stock | $66.90 | |
| Ab112659-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $199.90 | |
| Ab112659-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $299.90 | |
| Ab112659-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,299.90 |
Recombinant; Rabbit anti Human Lamin B1 Antibody; WB, IHC; Unconjugated
| Product Name | Recombinant Lamin B1 Antibody - Primary antibody, specific to LMNB1, Rabbit IgG |
|---|---|
| Synonyms | ADLD antibody; lamin B1 antibody; Lamin-B1 antibody; LMN antibody; LMN2 antibody; LMNB antibody; Lmnb1 antibody; LMNB1_HUMAN antibody; MGC111419 antibody; OTTHUMP00000159218 antibody; ADLD; Lamin B1; lamin-B1; LMN; LMN2; LMNB; LMNB1; MGC111419; MCPH26; La |
| Specifications & Purity | ExactAb™, Validated, Recombinant, High performance, 0.3 mg/mL |
| Host species | Rabbit |
| Specificity | LMNB1 |
| Immunogen | A synthetic peptide derived from human Lamin B1 (AA 527-540). |
| Positive Control | WB: A549, K562, THP-1, C6, NIH/3T3, Raw264.7, MCF7 and HeLa cell lysates. IHC: Human Lung cancer tissue. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, High performance, Recombinant, Validated |
| Product Description |
Rabbit anti Human Lamin B1 Antibody, Recombinant, could be used for WB, IHC and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant Lamin B1 Antibody (Ab112659) - Western Blot
All lanes: Recombinant Lamin B1 Antibody (Ab112659) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 66 kDa
Observed band size: 66, 22 kDa
Exposure time: 0.9 s
Recombinant Lamin B1 Antibody (Ab112659) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant Lamin B1 Antibody (Ab112659). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/2000 - 1/10000 |
| IHC | 1/50-1/200 |
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0404258 | Certificate of Analysis | Apr 09, 2024 | Ab112659 |
| ZJ23F1001427 | Certificate of Analysis | Oct 26, 2023 | Ab112659 |
| ZJ23F1001428 | Certificate of Analysis | Oct 26, 2023 | Ab112659 |
| 1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] |
| 2. Francis Impens,Lilliana Radoshevich,Pascale Cossart,David Ribet. (2014-08-13) Mapping of SUMO sites and analysis of SUMOylation changes induced by external stimuli.. Proceedings of the National Academy of Sciences of the United States of America, 111 ((34)): (12432-12437). [PMID:25114211] |
| 3. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 4. Pollard, K M KM and 5 more authors.. (1990) In vitro posttranslational modification of lamin B cloned from a human T-cell line.. Molecular and cellular biology, [PMID:2325650] |
| 5. Lin, F F and Worman, H J HJ.. (1995) Structural organization of the human gene (LMNB1) encoding nuclear lamin B1.. Genomics, (20): [PMID:7557986] |
| 6. Andersen, Jens S JS and 5 more authors.. (2003) Proteomic characterization of the human centrosome by protein correlation profiling.. Nature, (4): [PMID:14654843] |
| 7. Padiath, Quasar S QS and 8 more authors.. (2006) Lamin B1 duplications cause autosomal dominant leukodystrophy.. Nature genetics, [PMID:16951681] |
| 8. Beausoleil, Sean A SA, Villén, Judit J, Gerber, Scott A SA, Rush, John J and Gygi, Steven P SP.. (2006) A probability-based approach for high-throughput protein phosphorylation analysis and site localization.. Nature biotechnology, [PMID:16964243] |
| 9. Daub, Henrik H and 9 more authors.. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.. Molecular cell, (8): [PMID:18691976] |
| 10. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 11. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 12. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895] |
| 13. Ruan, Jianbin J and 7 more authors.. (2012) Crystal structures of the coil 2B fragment and the globular tail domain of human lamin B1.. FEBS letters, (17): [PMID:22265972] |
| 14. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 15. Guo, Ailan and 15 more authors.. (2014) Immunoaffinity enrichment and mass spectrometry analysis of protein methylation.. Molecular & cellular proteomics : MCP, [PMID:24129315] |
| 16. Johnson, Janel O JO and 41 more authors.. (2014) Mutations in the Matrin 3 gene cause familial amyotrophic lateral sclerosis.. Nature neuroscience, [PMID:24686783] |
| 17. Yeh, Chung-Hsin CH and 8 more authors.. (2015) SEPT12/SPAG4/LAMINB1 complexes are required for maintaining the integrity of the nuclear envelope in postmeiotic male germ cells.. PloS one, [PMID:25775403] |
| 18. Cristofoli, Francesca and 19 more authors.. (2020) De Novo Variants in LMNB1 Cause Pronounced Syndromic Microcephaly and Disruption of Nuclear Envelope Integrity.. American journal of human genetics, (1): [PMID:32910914] |
| 19. Parry, David A and 17 more authors.. (2021) Heterozygous lamin B1 and lamin B2 variants cause primary microcephaly and define a novel laminopathy.. Genetics in medicine : official journal of the American College of Medical Genetics, [PMID:33033404] |
