Recombinant; Rabbit anti Human LC3B Antibody; WB; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab112866-10μl | 10μl | In stock | $76.90 | |
| Ab112866-50μl | 50μl | In stock | $219.90 | |
| Ab112866-100μl | 100μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $339.90 | |
| Ab112866-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,809.90 |
Recombinant; Rabbit anti Human LC3B Antibody; WB; Unconjugated
| Product Name | Recombinant LC3B Antibody - Primary antibody, specific to LC3B, Rabbit IgG |
|---|---|
| Synonyms | ATG8F antibody|Autophagy-related protein LC3 B antibody|Autophagy-related ubiquitin-like modifier LC3 B antibody|LC3B antibody|LC3II antibody|MAP1 light chain 3 like protein 2 antibody|MAP1 light chain 3-like protein 2 antibody|MAP1A/1BLC3 antibody|MAP1A/ |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.8 mg/mL |
| Host species | Rabbit |
| Specificity | LC3B |
| Immunogen | A synthetic peptide derived from human LC3B (AA 1-110). |
| Positive Control | WB: HeLa, A549, MCF7, Raji, RAW 264.7 and C6 cell lysates. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human LC3B Antibody, Recombinant, could be used for WB and so on. Application: WB: 1/500-1/1000 Protein Function Probably involved in formation of autophagosomal vacuoles (autophagosomes). |
| Isotype | Rabbit IgG |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Form | Liquid |
| Concentration | 0.8 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant LC3B Antibody (Ab112866) - Western Blot
All lanes: Recombinant LC3B Antibody (Ab112866) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 15 kDa
Observed band size: 15, 17 kDa
Exposure time: 42.3 s
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB |
1/500-1/1000
|
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| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ24F0303020 | Certificate of Analysis | Mar 05, 2024 | Ab112866 |
| ZJ24F0303019 | Certificate of Analysis | Mar 05, 2024 | Ab112866 |
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| 2. He, Hua H and 12 more authors.. (2003) Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B.. The Journal of biological chemistry, (1): [PMID:12740394] |
| 3. Difilippantonio, S S and 8 more authors.. (2003) Gene expression profiles in human non-small and small-cell lung cancers.. European journal of cancer (Oxford, England : 1990), [PMID:12932674] |
| 4. Yang, Y-S YS and 8 more authors.. (2003) The gene expression profiling of sporadic pheochromocytoma and novel full-length cDNAs cloning.. Endocrine-related cancer, [PMID:14713272] |
| 5. Tanida, Isei I and 5 more authors.. (2004) HsAtg4B/HsApg4B/autophagin-1 cleaves the carboxyl termini of three human Atg8 homologues and delipidates microtubule-associated protein light chain 3- and GABAA receptor-associated protein-phospholipid conjugates.. The Journal of biological chemistry, (27): [PMID:15187094] |
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| 7. Tanida, Isei I, Ueno, Takashi T and Kominami, Eiki E.. (2004) Human light chain 3/MAP1LC3B is cleaved at its carboxyl-terminal Met121 to expose Gly120 for lipidation and targeting to autophagosomal membranes.. The Journal of biological chemistry, (12): [PMID:15355958] |
| 8. Kouno, Takahide T and 9 more authors.. (2005) Solution structure of microtubule-associated protein light chain 3 and identification of its functional subdomains.. The Journal of biological chemistry, (1): [PMID:15857831] |
| 9. Pankiv, Serhiy S and 8 more authors.. (2007) p62/SQSTM1 binds directly to Atg8/LC3 to facilitate degradation of ubiquitinated protein aggregates by autophagy.. The Journal of biological chemistry, (17): [PMID:17580304] |
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| 16. Sentelle, R David and 11 more authors.. (2012) Ceramide targets autophagosomes to mitochondria and induces lethal mitophagy.. Nature chemical biology, [PMID:22922758] |
| 17. Colecchia, David D and 8 more authors.. (2012) MAPK15/ERK8 stimulates autophagy by interacting with LC3 and GABARAP proteins.. Autophagy, [PMID:22948227] |
| 18. Choy, Augustine A and 6 more authors.. (2012) The Legionella effector RavZ inhibits host autophagy through irreversible Atg8 deconjugation.. Science (New York, N.Y.), (23): [PMID:23112293] |
| 19. Zhu, Yanyan Y and 9 more authors.. (2013) Modulation of serines 17 and 24 in the LC3-interacting region of Bnip3 determines pro-survival mitophagy versus apoptosis.. The Journal of biological chemistry, (11): [PMID:23209295] |
| 20. Lee, Dong Yun DY, Arnott, David D and Brown, Eric J EJ.. (2013) Ubiquilin4 is an adaptor protein that recruits Ubiquilin1 to the autophagy machinery.. EMBO reports, [PMID:23459205] |
| 21. Liu, Chao C and 5 more authors.. (2013) Arginine68 is an essential residue for the C-terminal cleavage of human Atg8 family proteins.. BMC cell biology, (30): [PMID:23721406] |
| 22. Tang, Zaiming Z and 7 more authors.. (2013) Autophagy promotes primary ciliogenesis by removing OFD1 from centriolar satellites.. Nature, (10): [PMID:24089205] |
| 23. Suzuki, Hironori H and 7 more authors.. (2014) Structural basis of the autophagy-related LC3/Atg13 LIR complex: recognition and interaction mechanism.. Structure (London, England : 1993), (7): [PMID:24290141] |
| 24. Lystad, Alf Håkon AH and 11 more authors.. (2014) Structural determinants in GABARAP required for the selective binding and recruitment of ALFY to LC3B-positive structures.. EMBO reports, [PMID:24668264] |
| 25. Strappazzon, F F and 10 more authors.. (2015) AMBRA1 is able to induce mitophagy via LC3 binding, regardless of PARKIN and p62/SQSTM1.. Cell death and differentiation, [PMID:25215947] |
| 26. Genau, Heide Marika HM and 7 more authors.. (2015) CUL3-KBTBD6/KBTBD7 ubiquitin ligase cooperates with GABARAP proteins to spatially restrict TIAM1-RAC1 signaling.. Molecular cell, (19): [PMID:25684205] |
| 27. Khaminets, Aliaksandr A and 17 more authors.. (2015) Regulation of endoplasmic reticulum turnover by selective autophagy.. Nature, (18): [PMID:26040720] |
| 28. Tumbarello, David A DA and 6 more authors.. (2015) The Autophagy Receptor TAX1BP1 and the Molecular Motor Myosin VI Are Required for Clearance of Salmonella Typhimurium by Autophagy.. PLoS pathogens, [PMID:26451915] |
| 29. Wei, Yongjie Y, Chiang, Wei-Chung WC, Sumpter, Rhea R, Mishra, Prashant P and Levine, Beth B.. (2017) Prohibitin 2 Is an Inner Mitochondrial Membrane Mitophagy Receptor.. Cell, (12): [PMID:28017329] |
| 30. Yang, Aimin A, Pantoom, Supansa S and Wu, Yao-Wen YW.. (2017) Elucidation of the anti-autophagy mechanism of the Legionella effector RavZ using semisynthetic LC3 proteins.. eLife, (11): [PMID:28395732] |
| 31. Kwon, Do Hoon DH and 6 more authors.. (2017) A novel conformation of the LC3-interacting region motif revealed by the structure of a complex between LC3B and RavZ.. Biochemical and biophysical research communications, (26): [PMID:28668392] |
| 32. Jena, Kautilya Kumar and 11 more authors.. (2018) TRIM16 controls assembly and degradation of protein aggregates by modulating the p62-NRF2 axis and autophagy.. The EMBO journal, (14): [PMID:30143514] |
