Recombinant; Rabbit anti Human LEF1 Antibody; WB, ICC, IF; Unconjugated
| SKU | Size | Availability | Price | Qty |
|---|---|---|---|---|
| Ab112963-10μl | 10μl | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $56.90 | |
| Ab112963-50μl | 50μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $169.90 | |
| Ab112963-100μl | 100μl | Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience! | $249.90 | |
| Ab112963-1ml | 1ml | Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding. | $2,299.90 |
Recombinant; Rabbit anti Human LEF1 Antibody; WB, ICC, IF; Unconjugated
| Product Name | Recombinant LEF1 Antibody - Primary antibody, specific to LEF1, Rabbit IgG |
|---|---|
| Synonyms | Anti-LEF1 antibody; DKFZp586H0919 antibody; FLJ46390 antibody; LEF 1 antibody; LEF-1 antibody; Lef1 antibody; LEF1_HUMAN antibody; Lymphoid enhancer binding factor 1antibody; Lymphoid enhancer-binding factor 1 antibody; T cell specific transcription facto |
| Specifications & Purity | ExactAb™, Validated, Recombinant, 0.1 mg/mL |
| Host species | Rabbit |
| Specificity | LEF1 |
| Immunogen | A synthetic peptide derived from human LEF1 (AA 269-282). |
| Positive Control | WB: Jurkat, K562, HEK293 and C6 cell lysates. ICC/IF: Jurkat cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Recombinant, Validated |
| Product Description | Rabbit anti Human LEF1 Antibody, Recombinant, could be used for WB, ICC, IF and so on. |
| Isotype | Rabbit IgG |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Antigen affinity purified |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
Recombinant LEF1 Antibody (Ab112963) - Western Blot
All lanes: Recombinant LEF1 Antibody (Ab112963) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 25-58 kDa
Exposure time: 150.0 s
Recombinant LEF1 Antibody (Ab112963) - Western Blot
All lanes: Recombinant LEF1 Antibody (Ab112963) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 44 kDa
Observed band size: 25-58 kDa
Exposure time: 150.0 s
Recombinant LEF1 Antibody (Ab112963) - IF/ICC
Immunocytochemistry analysis of LEF1 (green) in Jurkat cells using Recombinant LEF1 Antibody (Ab112963), and cells were counterstained with DAPI (blue).
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| ICC/IF |
1/50-1/200
|
Enter Lot Number to search for COA:
To view the certificate results,please click on a Lot number.For Lot numbers from past orders,please use our order status section
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ23F1001429 | Certificate of Analysis | Oct 26, 2023 | Ab112963 |
| ZJ23F1001430 | Certificate of Analysis | Oct 26, 2023 | Ab112963 |
